Marine fungi penicillium thomii, extract and application thereof

A technology of Penicillium tomosa and marine fungi is applied in the field of preparation of antibacterial and biological pesticides, which can solve problems such as difficulty and serious drug resistance.

Inactive Publication Date: 2012-06-13
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Terrestrial microorganisms have long been an important resource for medicine and pesticides. However, with the continuous development of terrestrial resources in recent years, it has become more and more difficult to screen new microbial resources and find new active metabolites, and the problem of drug resistance has become increasingly serious , making the development of new drugs face severe challenges

Method used

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  • Marine fungi penicillium thomii, extract and application thereof
  • Marine fungi penicillium thomii, extract and application thereof
  • Marine fungi penicillium thomii, extract and application thereof

Examples

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Effect test

Embodiment 1

[0021] Embodiment 1: Isolation and identification of marine fungus Tom Penicillium (Penicillium thomii) FS77

[0022] The marine fungus Penicillium thomii (Penicillium thomii) FS77 was isolated and purified from sea mud at a depth of 1675m in the South China Sea (106°30.295′E, 10°0.941′N). The separation and purification method is as follows: dilute the sea mud sample to 10% with sterile water containing 30% coarse sea salt, shake at 27°C for 20 minutes, take 0.1mL and evenly spread it on the improved PDA solid medium, and place it in a thermostat at 26-28°C Cultivate, and after the colony grows out, pick the colony and streak to purify to obtain a strain. The improved PDA solid medium is to add KH in every liter of PDA medium 2 PO 4 3g, MgSO 4 0.75g, vitamin B 1 10mg, coarse sea salt 30g, agar 20g, and the balance is water.

[0023]Through morphological studies, and according to the "Fungi Identification Manual" (Wei Jingchao, 1979), the strain was identified as Penicill...

Embodiment 2

[0026] Embodiment 2: Preparation of marine fungus Penicillium thomii (Penicillium thomii) FS77 fermentation extract

[0027] The marine fungus Penicillium thomii (Penicillium thomii) FS77 was inoculated into the improved PDA liquid medium, cultured at 120r / min, 26°C for 7 days, and the liquid culture of the marine fungus Penicillium thomii (Penicillium thomii) FS77 was obtained. The liquid culture of the marine fungus Penicillium thomii (Penicillium thomii) FS77 is filtered to separate the mycelia from the fermentation broth, the fermentation broth is extracted three times with an equal volume of ethyl acetate, the extracts are combined, and the extracts are then concentrated under reduced pressure to obtain That is, a brown paste-like extract is obtained, which is the fermented extract of the marine fungus Penicillium thomii (Penicillium thomii) FS77.

[0028] The improved PDA liquid medium is obtained by the following method: boil 200g of potatoes with 500mL of distilled wat...

Embodiment 3

[0029] Embodiment 3: the antibacterial activity of marine fungus Penicillium thomii (Penicillium thomii) FS77 fermentation extract

[0030] one,

[0031] The inhibitory effect of the marine fungus Penicillium thomii FS77 fermented extract on Bacillus subtilis, Staphyloccocus aureus and Pseudomonas aeruginosa was determined by the filter paper method.

[0032] The marine fungus Penicillium thomii (Penicillium thomii) FS77 fermentation extract obtained in Example 2 was dissolved in DMSO and diluted to a concentration of 50 mg / mL.

[0033] The above-mentioned bacterial liquid to be tested obtained by liquid fermentation was diluted to 10 with physiological saline respectively. 6 The concentration of CFU / mL, the absorption concentration is 10 6 200 μL of CFU / mL bacterial solution was evenly spread on the plate containing LB medium, and a filter paper sheet with a diameter of 4 mm was attached, and the marine fungus Penicillium thomii (Penicillium thomii) FS77 with a concentratio...

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Abstract

The invention discloses a marine fungi penicillium thomii, an extract and application thereof. A marine fungi penicillium thomii (penicillium thomii) FS77 was preserved at China Center for Type Culture Collection (CCTCC) at Wuhan University of Wuhan City of China on November 24th, 2011 and the preserving number is CCTCC M201414. The fermentation extract has restraining effects on staphyloccocus aureus, bacillus subtilis and pseu domonas aeruginosa. Simultaneously, the fermentation extract has resisting effects on plant pathogen fungi alternaria alternate, colletotrichum gloeosporioides and cylindrocladium scoparium. Accordingly, the marine fungi penicillium thomii provides selectable chemicals for studying and developing new antibacterial chemicals and biopesticides, and provides scientific evidences for developing natural active materials of a marine fungi source.

Description

Technical field: [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a marine fungus Penicillium thomii (Penicillium thomii) FS77, and a fermented extract prepared from the liquid culture fermentation broth of the strain, and the extract used in the preparation of antibacterial and biological pesticides in the application. Background technique: [0002] Terrestrial microorganisms have long been an important resource for medicine and pesticides. However, with the continuous development of terrestrial resources in recent years, it has become more and more difficult to screen new microbial resources and find new active metabolites, and the problem of drug resistance has become increasingly serious , making the development of new drugs face severe challenges. The ocean accounts for about 71% of the earth's surface area, and contains rich microbial resources. The special marine environment (low temperature, high pressure, high salinit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61K36/06A61P31/04A01N63/04A01P3/00C12R1/80
Inventor 李浩华章卫民王磊陈玉婵
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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