Isocoumarin compound as well as preparation method and application of isocoumarin compound as natural marine organism antifouling agent

A technology of isocoumarin and compounds, applied in the direction of biochemical equipment and methods, biocides, and methods based on microorganisms, can solve the problems of destroying the marine environment, destroying the immune system, endangering human health, etc., and achieves a broad application prospect Effect

Active Publication Date: 2014-12-24
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These anti-fouling materials have also seriously damaged the marine environment while playing an anti-fouling role. For example, organotin oxides (TBTO) have damaged the immune systems of many fish and marine organisms, and pesticides have fouled the target. Both biological and non-target organisms produce toxicity and directly endanger human health

Method used

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  • Isocoumarin compound as well as preparation method and application of isocoumarin compound as natural marine organism antifouling agent
  • Isocoumarin compound as well as preparation method and application of isocoumarin compound as natural marine organism antifouling agent
  • Isocoumarin compound as well as preparation method and application of isocoumarin compound as natural marine organism antifouling agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Culture of marine fungus Eurotium sp. (HM991283)

[0017] The medium used for the culture of the fungus Eurotium sp. (HM991283) contains 1.0% glucose (percentage by weight, the same below), 0.1% yeast extract, 0.2% peptone, 1.0% agar, 3% coarse sea salt, and the rest is water. When making a test tube slant, the fungal strains were cultured at 28°C for 5 days.

[0018] (2) Fermentation of marine fungus Eurotium sp. (HM991283)

[0019] The fermentation medium used for the fermentation culture of the fungus Eurotium sp. (HM991283) contains glucose 1.0% (percentage by weight, the same below), yeast extract 0.1%, peptone 0.2%, coarse sea salt 3.0%, and the rest is water. Cultivate at 30°C.

[0020] (3) separation and extraction of formula I compound of the present invention

[0021] Get 100L of fermented product of step (2) gained, after fermented liquid and thalline are separated, fermented liquid is extracted 5 times with ethyl acetate, extract is concentrated under...

Embodiment 2

[0030] (1) Culture of marine fungus Eurotium sp. (HM991283)

[0031]The strain medium contains glucose 0.1%-5.0% (weight percentage, the same below), yeast extract 0.01%-2%, peptone 0.01%-2%, agar 0.1%-3.0%, coarse sea salt 0.05%-5%, and the rest It is water; the cultivation temperature is 5-45° C.; the cultivation time is 3-10 days.

[0032] (2) Fermentation of marine fungus Eurotium sp. (HM991283)

[0033] The fermentation medium contains 0.1%-5.0% of glucose (percentage by weight, the same below), 0.01%-2% of yeast extract, 0.01%-2% of peptone, 0.05%-5% of coarse sea salt, and the rest is water; the culture temperature is 5- 45°C; culture time is 5-50 days.

[0034] (3) separation and extraction of formula I compound of the present invention

[0035] Get 50L of the fermented product obtained in step (2), separate the fermented liquid from the thalline, extract the fermented liquid 2 to 6 times with ethyl acetate, concentrate the extract under reduced pressure to obtain t...

Embodiment 3

[0038] The marine fungus Eurotium sp. (HM991283) was cultured in the strain medium, and then the above-mentioned marine fungus was fermented and cultured in the fermentation medium. After the fermentation liquid and the bacteria were separated, the fermentation liquid was extracted with ethyl acetate , the extract was concentrated under reduced pressure to obtain a fermented liquid extract; the cells were extracted with methanol and concentrated under reduced pressure to obtain a cell extract; (Compound 1-12) is the compound of formula I, and its structure confirmation data is consistent with the corresponding data in Example 1. Wherein the strain medium contains glucose, yeast extract, peptone, agar, coarse sea salt, water, and the fermentation medium contains glucose, yeast extract, peptone, coarse sea salt, water; the chromatographic separation is normal phase Silica gel column chromatography and high performance liquid chiral chromatography.

[0039] In order to explore a...

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Abstract

The invention discloses an isocoumarin compound as well as a preparation method and application of the isocoumarin compound as a natural marine organism antifouling agent. The isocoumarin compound is characterized in that the compound has a structure as shown in formula I, wherein R2 and R2 independently represent H and a structure as shown in specification, and R1 or R2 is H; and R3 and R4 independently represent H and OCH3, and R3 or R4 is H. The compound as shown in formula I can be prepared through adopting such means as cultivation, fermentation and chromatographic separation on marine fungi, i.e., Eurotium sp. (HM991283). The natural marine organism antifouling agent disclosed by the invention is efficient and low in toxicity, and is characterized in that the compound of formula I or salt thereof, as an active ingredient, is used for preventing and controlling marine organism fouling caused by barnacle.

Description

technical field [0001] The invention relates to an isocoumarin compound, in particular to a preparation method of the isocoumarin compound with the activity of inhibiting the attachment of barnacle larvae and its application as a natural marine biological antifouling agent. Background technique [0002] Marine biofouling means that the larvae of marine fouling organisms such as barnacles, mussels, and bryozoans attach and grow on the surface of marine facilities such as hulls, fish tanks, oil wells, or other marine organisms to form communities, thereby causing huge damage to the attached objects. Damage, such as accelerating metal corrosion, reducing the performance of marine facilities, affecting the output and quality of aquaculture, etc. Among marine fouling organisms, barnacles are the most widely distributed and most harmful. The economic loss caused by marine biofouling is huge every year all over the world. Taking ships as an example, fouling organisms adhere to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/76A01P7/00C12P17/06C12R1/645
CPCC07D311/76C12P17/06
Inventor 王长云邵长伦陈敏王开玲
Owner OCEAN UNIV OF CHINA
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