A Pseudomonas cepacia strain qba-3 with inhibitory effect on Botrytis cinerea and its application

A technology of Pseudomonas cepacia and Botrytis cinerea, applied in the field of microorganisms, can solve problems that affect the sustainable and healthy development of agriculture, destroy ecological balance, and endanger the health of consumers, and achieve good market application prospects

Inactive Publication Date: 2017-12-08
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Botrytis cinerea reproduces very fast and produces a large number of conidia, so it is easy to mutate and produce drug resistance. At present, it has been used in agricultural production for benzimazoles, dicarboximides, and carbamates. Resistance to various fungicides such as fungicides, which not only leads to the reduction of control efficiency, but also leads to the continuous increase of the amount of pesticides used
Excessive use of chemical agents not only causes excessive pesticide residues in agricultural products, endangers the health of consumers, but also causes environmental pollution, destroys ecological balance, and affects the sustainable and healthy development of agriculture.

Method used

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  • A Pseudomonas cepacia strain qba-3 with inhibitory effect on Botrytis cinerea and its application
  • A Pseudomonas cepacia strain qba-3 with inhibitory effect on Botrytis cinerea and its application
  • A Pseudomonas cepacia strain qba-3 with inhibitory effect on Botrytis cinerea and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0020] 1. Isolation and screening of strains

[0021] On March 19, 2013, 20 tomato leaf samples were collected in a greenhouse in Laiyang City. Each leaf was soaked in 75% alcohol for 10 seconds, then soaked in 0.1% mercury chloride for 30 seconds, rinsed with sterile water for 3 times, and transferred to sterilized water. Grind in a mortar treated with bacteria, add sterile water to dilute to 10ml, continue to dilute to 10ml with sterile water -5 times, take 100ul respectively and apply LB plates (tryptone 10 g / L, yeast extract 5 g / L, sodium chloride 5 g / L, pH 7.0), and culture at 30°C.

[0022] Pick a single colony and inoculate a single bacterial colony in YEPD medium (yeast extract 3 g / L, peptone 10 g / L, glucose 20 g / L) at 25°C 120 rpm for 24 hours, then culture at 25°C 12000 rpm Centrifuge under the conditions, filter the supernatant through a filter with a diameter of 0.22um, inoculate the conidia of Botrytis cinerea into the filtrate and let it stand for cultivation, i...

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Abstract

The invention discloses a Pseudomonas cepacia strain QBA-3 which has an inhibitory effect on Botrytis cinerea and its application. The QBA-3 obtained by screening in the present invention is classified as Pseudomonas cepacia and is preserved in China Center for Type Culture Collection, its collection number is CCTCC M 2015402. The present invention proves that the bacterial strain QBA-3 obtained by the present invention has a strong inhibitory effect on Botrytis cinerea through experiments on Botrytis cinerea spore germination, germ tube elongation, and isolated leaves, and can be prepared as a product for preventing and treating Botrytis cinerea. Biocontrol agents have good market application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a Pseudomonas cepacia strain QBA-3 capable of inhibiting Botrytis cinerea and its application. Background technique [0002] Botrytis cinerea is an important plant pathogenic fungus widely distributed in the world. There are many kinds of hosts of this fungus, which can infect the stems, leaves, flowers and fruits of 235 kinds of plants, such as tomatoes and cucumbers, and grapes and strawberries. mildew. The disease can occur not only during the growing season of the host plant, but also during the storage of agricultural products, so the damage is serious. Especially in recent years, the large-scale promotion of protected cultivation methods has led to wide sources of infection and rapid spread of the fungus. Botrytis cinerea is becoming more and more serious, and its occurrence and prevalence often cause serious economic losses. According to reports, the occurre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/38
CPCA01N63/10C12N1/205C12R2001/38
Inventor 周善跃高攀梁文星李保华李宝笃
Owner QINGDAO AGRI UNIV
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