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A kind of lignan compound and its preparation method and application as natural marine biological antifouling agent

A compound and lignan technology, applied in the field of lignan compounds, can solve problems such as marine biological antifouling agents that have not yet been seen, and achieve the effect of broad application prospects

Active Publication Date: 2016-03-02
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no marine biological antifouling agent with important application value has been obtained from marine microorganisms

Method used

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  • A kind of lignan compound and its preparation method and application as natural marine biological antifouling agent
  • A kind of lignan compound and its preparation method and application as natural marine biological antifouling agent
  • A kind of lignan compound and its preparation method and application as natural marine biological antifouling agent

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Embodiment Construction

[0009] Preparation of Compounds of the Invention

[0010] (1) The medium used for the culture of the fungus Aspergillus sp. (HM991281) contains 1.0% glucose (percentage by weight, the same below), 0.1% yeast extract, 0.2% peptone, 1.0% agar, 3% coarse sea salt, and the rest is water , when used to make a test tube slant, fungal strains were cultured at 28 ° C for 5 days.

[0011] The strain culture medium contains 0.1%-5.0% of glucose (percentage by weight, the same below), 0.01%-2% of yeast extract, 0.01%-2% of peptone, 0.1%-3.0% of agar, and 0.05%-5% of coarse sea salt. %, the rest is water; the culture temperature is 5-45°C; the culture time is 3-10 days.

[0012] (2) The fermentation medium used for the fermentation culture of the fungus Aspergillus sp. (HM991281) contains 1.0% glucose (percentage by weight, the same below), 0.1% yeast extract, 0.2% peptone, 3.0% coarse sea salt, and the rest is water. Cultured at 25°C for 30 days.

[0013] The fermentation medium conta...

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Abstract

The invention relates to methybutyrolactone II and a preparation method thereof, and an application of the methybutyrolactone II as a nature marine organism antifouling agent. During preparation, bacterial culture is performed on Aspergillus sp.(HM991281) in a strain culture medium, fermentation culture is performed on fungus in a fermentation culture medium, the obtained fermentation broth is filtered to remove thallus, the filtrate is concentrated, extraction is performed by using ethyl acetate, the extraction solution is subjected to pressure reduction concentration and chromatographic separation, and the obtained eluent is concentrated to obtain a light yellow oily substance, wherein the light yellow oily substance is methybutyrolactone II. The present invention provides an efficient and low toxicity nature marine organism antifouling agent, which is characterized in that the methybutyrolactone II is adopted as an active component for prevention and control of marine organism fouling caused by Balanusamphitrite larva attachment, and the raw material can be produced in a large-scale manner through fungus fermentation and is not limited by resources, such that application prospects are broad.

Description

technical field [0001] The present invention relates to a lignan compound whose 2-hydroxyl group is methylated which is rare in nature, in particular to a preparation method of a lignan compound having the activity of inhibiting the attachment of barnacle larvae and its application as an antifouling agent for marine organisms . Background technique [0002] Marine biofouling means that the larvae of marine fouling organisms such as barnacles, mussels, and bryozoans attach and grow on the surface of marine facilities such as hulls, fish tanks, oil wells, or other marine organisms to form communities, thereby causing huge damage to the attached objects. Damage, such as accelerating metal corrosion, reducing the performance of marine facilities, affecting the output and quality of aquaculture, etc. Among marine fouling organisms, barnacles are the most widely distributed and most harmful. The economic loss caused by marine biofouling is huge every year all over the world. Ta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/68C12P17/04C12R1/66C09D5/16
Inventor 王长云邵长伦陈敏
Owner OCEAN UNIV OF CHINA
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