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Schizochytrium LX0809 (marine fungus) and industrial application thereof

A technology of marine fungi and Schizochytrium, applied in the fields of application, fungi, animal husbandry, etc., can solve the problem of low production cost, achieve the effect of simple long-chain polyunsaturated fatty acid spectrum, and facilitate the separation and purification of DHA

Inactive Publication Date: 2012-10-03
吉林省希玛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production cost of fish oil DHA is low and the price is cheap, but fish oil often contains harmful substances such as DDT, chemical pesticides, PCBs, dioxins and hexachlorobenzene

Method used

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  • Schizochytrium LX0809 (marine fungus) and industrial application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Implementation Example 1. Screening of Schizochytrium

[0017] Get the mulch collected in the red beach of Zhaoquanhe Township, Dawa County, Panjin City, Liaoning Province, inoculate it on the described primary screening medium plate, and enrich and cultivate it for 5 days at 30°C. Pick the culture and transfer it to a new plate for cultivation until a pure culture is obtained. The pure culture was inoculated into a 250ml shake flask filled with 50ml of the re-screened medium, and cultivated on a shaker at 200rpm for 3 days at a culture temperature of 30°C. The culture solution was centrifuged (8000 rpm, 5 minutes), washed with water, then centrifuged, then vacuum-dried (at a temperature of 60-80° C. and a vacuum of 80 kPa) for 3 hours, and the dry weight of the cells was weighed. The oil in dry cell powder was extracted by Soxhlet extraction, and then the fatty acid composition in the oil was detected by gas chromatography. Taking the yield of DHA as an index, the Sch...

Embodiment 2

[0021] Implementation example 2. Fermentative production of Schizochytrium (Schizochytrium) LX0809

[0022]The Schizochytrium LX0809 strain grown on the slope for 45 hours was inoculated into a 500 ml shake flask. Seed liquid and fermentation medium include 50g / L glucose, 10g / L corn steep liquor, 5g / L sodium chloride, 2g / L potassium chloride, 0.5g / L magnesium sulfate, 0.05g / L manganese chloride, 0.01g / L L boric acid, 0.01g / L potassium bromide. Cultivate in a shaker at 30°C at a speed of 250rpm until the OD600 of the bacterial solution reaches 1.0 to complete the first-generation activation, then take 10ml of the bacterial solution and inoculate it into the above-mentioned fresh medium, and continue for three generations (all until the OD600 of the bacterial solution reaches When 1.0 is completed as a generation of activation), obtain the seed liquid with higher vigor; Seed liquid is inoculated to the fermenter of 5L with 1% inoculum size again, stirring speed 300rpm, air flow...

Embodiment 3

[0026] Implementation example 3. Fermentative production of Schizochytrium (Schizochytrium) LX0809

[0027] The Schizochytrium LX0809 strain grown on the slope for 45 hours was activated according to the method in Example 2, and then 5% of the inoculum was inoculated into a 5L fermenter. The fermentation medium is: 30g / L glucose, 1g / L corn steep liquor, 5g / L sodium chloride, 2g / L potassium chloride, 0.5g / L magnesium sulfate, 0.05g / L manganese chloride, 0.01g / L boric acid , 0.01g / L potassium bromide. Fermentation was stopped for 80 hours according to the conditions of Example 2, and the dry cell weight of the fermentation broth was measured to be 67.7g / L. Extract with normal hexane to obtain thalline fat 32ml / L. Get 1.0g of the extracted oil and place it in a 200ml round-bottomed flask, add 70.5ml of potassium hydroxide-methanol solution with a concentration of 0.8M, saponify under reflux in a water bath at 65°C for 15 minutes; add 70ml of 45% (w / v) Tris Boron fluoride-dieth...

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Abstract

The invention provides schizochytrium LX0809 (marine fungus), which has a high docosahexaenoic acid (DHA) production capability. The schizochytrium LX0809 of the invention was separated from the corroded soil collected from the red beach in Zhaoquanhe Town, Dawa County, Liaoning Province and was preserved in China General Microbiological Culture Collection Center with a preservation number CGMCC No.3535 on December 23th, 2009. When the schizochytrium LX0809 is used for fermenting supplementary food, the dry cell weight of the fermentation liquor can reach 72.5 g / L, and the DHA content can reach 15.3g / L. LX 0809 thallus cells and extracts thereof can be used as food additives for edible oil, dairy products, grain products and the like and can be used as a feed additive for aquaculture and livestock culture and the like. Saturated triglycercide in the thallus cells can be used to produce biodiesel through catalytic transesterification.

Description

technical field [0001] The invention relates to the application technology of marine microorganisms, in particular to a marine fungus Schizochytrium (Schizochytrium) LX0809 and its application in the industrial production of food and feed additives and biodiesel. It belongs to the technical field of microbial application. Background technique [0002] Nutritional and medical studies have shown that DHA is an important nutraceutical that can effectively improve health and resist disease. DHA is the main lipid component in the nerve cell membrane of the human brain, and is also an important fatty acid component in the brain, nerve and visual cells. It plays an important role in the normal performance of human physiological functions and the prevention and treatment of various diseases, especially in the brain and It plays an important role in the development of the visual system. DHA is involved in the development of infant brain tissues such as brain vesicles, myelin and mi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P7/64A23L1/30A23K1/16A23D7/00A23D9/00C11C3/10C12R1/645
Inventor 杨勇郗大兴
Owner 吉林省希玛生物科技有限公司
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