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Mutagenized Aspergillus unguis 6-20-6

A technology of marine fungi and mutagenic strains, applied in the direction of fungi, microorganisms, biochemical equipment and methods, etc., which can solve problems such as expensive, toxic and side effects, and loss of efficacy of antibiotics

Inactive Publication Date: 2018-11-06
GUANGDONG OCEAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, in clinical treatment, it is found that the drug resistance of pathogenic microorganisms is getting stronger and stronger, especially methoxypenicillin-resistant Staphylococcus aureus (super drug-resistant bacteria), Pseudomonas aeruginosa (commonly known as Pseudomonas aeruginosa), Arc Infections caused by bacteria and Candida albicans (commonly known as Candida albicans) are becoming more and more serious, and many traditional antibiotics have basically lost their efficacy
[0003] Moreover, pesticides such as pesticides are commonly used in agriculture. Due to the excessive use of various chemically synthesized pesticides in agricultural production, serious food, soil and water pollution are caused, and human health and ecological balance are endangered through the food chain. Pesticide residues also seriously hinder my country's The export of agricultural products; and biopesticides are not easy to produce drug resistance, safe for non-target organisms, environmentally friendly and easy to degrade naturally, which is of great significance to human health, environmental protection and sustainable agricultural development.
[0004] At the same time, the acetylcholinesterase inhibitors used in clinic are mainly physostigmine, galantamine, huperzine A, rivastigmine, tacrine and donepezil, but these drugs are expensive, and except for huperzine Alkaline A has certain toxic and side effects

Method used

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  • Mutagenized Aspergillus unguis 6-20-6
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  • Mutagenized Aspergillus unguis 6-20-6

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] This example is the preparation method of the marine fungus bromide phenolic acid cyclic ether compounds.

[0070] Step 1. Fermentation

[0071] The Aspergillus unguis 6-20-6 mutant strain of Aspergillus claw was inoculated in the fungal liquid culture medium and left to ferment at 28°C for 20 days, adding an appropriate amount of diatomaceous earth, filtering, and collecting mycelium and fermentation broth respectively.

[0072] Wherein, the formula of the fungal liquid culture medium is: each liter contains the following components: 500 mL of potato juice, 20 g of sea salt, 20 g of sucrose, and 500 mL of ultrapure water.

[0073] The potato juice is prepared by peeling the potatoes, washing them, adding deionized water, heating to boiling for 30 minutes, filtering, boiling 500 mL of potato juice for every 200 g of potatoes, and freezing for preservation.

[0074] Step 2. Rough extraction

[0075] The fermentation broth obtained in step 1 was absorbed by 300mL NKA re...

Embodiment 2

[0094] This example is an experiment on the activity of depsipphenolic acid cyclic ether compounds in inhibiting Gram-positive drug-resistant bacteria.

[0095] 1. Staphylococcus aureus is a very common bacteria, sometimes it will enter the human body and cause infection. The infection can range from mild sores and pimples on the skin to pneumonia or blood infection. Therefore, Staphylococcus aureus is an important target pathogenic microorganism for the development of new antibiotics.

[0096] 2. Experimental method:

[0097] The antimicrobial activity was initially screened using the international standard filter paper method, and the antimicrobial activity was measured by the diameter of the inhibition zone (Clinical and Laboratory Standards Institute.2012.Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard.Eleventh EditionM2-A11.); Sieve using a 96-well plate, adopt the international standard micro broth dilution method (National Committe...

Embodiment 3

[0102] This embodiment is an experiment on the activity of depsipphenolic acid cyclic ether compounds against Gram-negative bacteria.

[0103] 1. Pseudomonas aeruginosa is widely distributed in nature. This bacterium exists in all kinds of water, air, normal human skin, respiratory tract and intestinal tract. This bacterium often causes postoperative wound infection, and can also cause bedsores and abscesses , suppurative otitis media, etc.

[0104] Vibrio parahaemolyticus widely exists in the sea and in seafood, infection with this bacteria will cause acute onset, abdominal pain, vomiting, diarrhea and watery stool.

[0105] 2. Experimental method:

[0106] The antimicrobial activity was initially screened using the international standard filter paper method, and the antimicrobial activity was measured by the diameter of the inhibition zone (Clinical and Laboratory Standards Institute.2012.Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard.E...

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Abstract

The invention discloses mutagenized Aspergillus unguis 6-20-6, collected in Guangdong Microbial Culture Collection Center on May, 20, 2018 under GDMCC 60377; the collection address is Guangdong Institute of Microbiology, Fifth floor, No. 59 building, No. 100 Compound, Central Xianlie Road, Guangzhou. The mutagenized Aspergillus unguis 6-20-6 provided herein can generate active materials, compoundsA and B, having antibacterial, antifungal, insecticidal or acetylcholinesterase-inhibiting effects; the structural formulas of the compounds A and B are shown as formula 1 and formula 2 respectively;the mutagenized Aspergillus unguis 6-20-6 has a promising application prospect in the preparation of antifungal agents, antibacterial agents, agricultural insecticides or acetylcholinesterase inhibitors.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a marine fungus Aspergillus unguis 6-20-6 mutagenic strain. Background technique [0002] At present, in clinical treatment, it is found that the drug resistance of pathogenic microorganisms is getting stronger and stronger, especially methoxypenicillin-resistant Staphylococcus aureus (super drug-resistant bacteria), Pseudomonas aeruginosa (commonly known as Pseudomonas aeruginosa), Arc Infections caused by bacteria and Candida albicans (commonly known as Candida albicans) are becoming more and more serious, and many traditional antibiotics have basically lost their efficacy. [0003] Moreover, pesticides such as pesticides are commonly used in agriculture. Due to the excessive use of various chemically synthesized pesticides in agricultural production, serious food, soil and water pollution are caused, and human health and ecological balance are endangered through th...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/08C12R1/66
CPCC12N1/14C12P17/08C12N1/145C12R2001/66
Inventor 张翼杨文聪鲍海燕刘亚月聂影影杨静明洪鹏志千忠吉宋采梁金月黎钊坪
Owner GUANGDONG OCEAN UNIVERSITY
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