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Nannochloropsis oculata and application thereof

A kind of technology of pseudo-Ninochlorococcus and microbial strains, which is applied in the field of microorganisms

Active Publication Date: 2021-02-12
广西小藻农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although compared with other species of marine microalgae, the total EPA content of Nannochloropsis is at a higher level, but there is still a certain distance from the realization of large-scale industrial production and stable fine algae species

Method used

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  • Nannochloropsis oculata and application thereof
  • Nannochloropsis oculata and application thereof
  • Nannochloropsis oculata and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Quizalofop-p-ethyl for Screening Nannochloropsis

[0053] 1. The growth inhibition of quizalofop-p-ethyl to Nannochloropsis

[0054] (1) Weigh 10 mg quizalofop-p-p-p-p powder and dissolve in 1 mL DMSO to obtain 26.8 μmol / mL inducer mother solution.

[0055] (2) Add the inducer mother solution to the MASM improved liquid medium, and prepare the liquid with the final concentration of quizalofop-p-ethyl at 0, 2.5mmol / L, 5mmol / L, 7.5mmol / L, 10mmol / L and 12.5mmol / L The culture media are respectively recorded as Q0, Q1, Q2, Q3, Q4, and Q5 groups in sequence, wherein, the culture medium Q0 without adding quizalofop-p-p is used as a control.

[0056] (3) According to the ratio of adding 2 mL of algae species to 100 mL of liquid culture medium, inoculate the algae liquid into the culture solution of the prepared 6 gradient concentrations of the Erlenmeyer flask, seal with a gas-permeable membrane, and record the shake flask as F 1 generation. Place the inoculated Erlenmeyer f...

Embodiment 2

[0068] Growth Performance Test

[0069] Prepare six 1000mL bottles of culture solution, sterilize at 121°C for 30 minutes, and cool it for later use; add each nutrient mother solution in the biological safety cabinet according to the above-mentioned MASM improved liquid medium formula to make a liquid medium; after the culture solution is prepared, press 5% of its volume (about 50mL) was respectively inserted into the algae fluids of groups Q0, Q1, Q2, Q3, Q4, and Q5, and each two were parallelized. 3000~6000lux, the mixed gas of air and 1%~2% carbon dioxide is introduced, and the culture bottle is marked as F 2 generation. Take aseptic samples regularly, measure the change in optical density, and draw the change graph of growth curve. After 14 days of cultivation, the culture bottle was taken out to collect the algae fluid, and the specific growth rate was measured. The algae liquid was collected by centrifugation, and the inorganic salts were washed with pure water for 3 ...

Embodiment 3

[0086] This example provides a method for preparing fatty acids. The algae strain Q5 in Example 2 is inoculated through a photocatalytic reactor for large-scale cultivation. The cultured product is extracted with chloroform / methanol and the corresponding ARA and EPA are separated by chromatography. The Nannochloropsis has high fatty acid content, stable genetic traits, and can efficiently and rapidly obtain a large amount of fatty acid products.

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Abstract

The invention provides nannochloropsis oculata and application thereof. The nannochloropsis oculata is classified and named as Nannochloropsis salina, and is preserved in Guangdong province microbialstrain preservation center located in the fifth floor of the 59 # building of the 100 # courtyard of the Xianlie Middle Road of Guangzhou on October 30, 2020, and the preservation number is GDMCC No:61177. The nannochloropsis oculata disclosed by the embodiment of the invention at least has the following beneficial effects that compared with a wild nannochloropsis oculata strain, the fatty acid yield of the nannochloropsis oculata screened by the embodiment of the invention, especially the yield of eicosapentaenoic acid, is obviously improved, and the nannochloropsis oculata can be used as astable excellent algae species for large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to Nannochloropsis pseudochloropsis and its application. Background technique [0002] Eicosapentaenoic acid (EPA) is an omega-3 unsaturated fatty acid, which plays an important role in nutrition and pharmacology. It can not only promote brain development, improve brain function, etc., but also treat rheumatoid arthritis, etc. The prevention and treatment of inflammation, hypertension, diabetes and other diseases have obvious positive effects. Marine microalgae are primary producers of EPA and can utilize organic carbon sources for heterotrophic growth. The researchers found that, for example, Nannochloropsis sp. of Chrysophyta, Porphyridium cruentum of Rhodophyta, Phaeodactylum tricornutum of Diatoms, Monodus subterraneus ), Nitzschialaevis, etc. are good sources of EPA. The EPA production of marine microalgae can be further improved by optimizing the culture conditions, contro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C07C51/42C07C57/03A61K36/05A61P9/12A61P3/10A61P9/10A61P29/00C12R1/89
CPCC12P7/6427C07C51/42A61K36/05A61P9/12A61P3/10A61P9/10A61P29/00C12R2001/89C12N1/125C07C57/03
Inventor 李林乔瓦尼科利卡吴玉双胡桑秦捷王敬李文香俞威
Owner 广西小藻农业科技有限公司
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