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Establishment method and use of wild bluegrass AFLP fingerprint

A technology of wild bluegrass and fingerprints, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem of not finding relevant reports, etc., and achieve the effect of shortening the detection cycle

Inactive Publication Date: 2014-07-02
GANSU AGRI UNIV
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Problems solved by technology

No relevant reports have been found on the research on the AFLP fingerprint of wild bluegrass

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  • Establishment method and use of wild bluegrass AFLP fingerprint
  • Establishment method and use of wild bluegrass AFLP fingerprint
  • Establishment method and use of wild bluegrass AFLP fingerprint

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Embodiment Construction

[0060] The technical scheme of the present invention will be further described below in conjunction with specific examples.

[0061]In the present invention, 39 wild bluegrass materials from Gansu Province and 4 wild bluegrass materials from Qinghai Province are planted by sand culture, the leaves are cut at the seedling stage, genomic DNA is extracted, and enzyme digestion, connection, pre-amplification, and selective amplification are carried out. , denatured polyacrylamide gel electrophoresis, and a large number of DNA bands with different heights were obtained. Poa annua germplasm with the same band is considered to have the same genetic locus, and Poa annua germplasm with DNA bands of different sizes is considered to be With different genetic loci, the fingerprints of different wild bluegrass germplasms are established, which are used for the identification, variety identification and germplasm protection of bluegrass germplasms.

[0062] 1. Wild bluegrass genomic DNA ext...

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Abstract

The invention discloses an establishment method and a use of wild bluegrass AFLP (Amplified Fragment Length Polymorphism) fingerprint. The establishment method comprises six steps of genome DNA (Deoxyribose Nucleic Acid) extraction, enzyme digestion, connection, pre-amplification, selective amplification and denaturing polyacrylamide gel electrophoresis, and then is capable of obtaining a large number of high and low different DNA strips; bluegrass germplasms having the DNA strips of same size are considered to have the same genetic loci and bluegrass germplasms having the DNA strips of different sizes are considered to have different genetic loci, and on this basis, the fingerprint of different wild bluegrass germplasms is established, and can be used for identifying the bluegrass germplasms, identifying variety and protecting the germplasms. The establishment method has the advantages that the genome DNAs of the bluegrass plants are directly detected and the influence on the phenotypic characters of the genome DNAs caused by factors such as the field environment is avoided, and in addition, the method can be used for detecting and identifying the genome DNAs at the seedling stage, and therefore, the detection period is shortened.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for establishing a turfgrass fingerprint and its application, in particular to a method for establishing a wild bluegrass AFLP fingerprint and its application. Background technique [0002] DNA molecular markers have been gradually developed since the 1980s. This technology uses various processing methods (restriction enzyme digestion, PCR amplification, molecular hybridization, and electrophoresis detection) to process genomic DNA between different groups or individuals. The polymorphism of DNA fragments can be displayed, and the marking, statistics and analysis of these polymorphic fragments can be used in various researches, such as genetic diversity, isolation and identification of germplasm resources, etc., at the DNA level Reflect the genetic differences between individuals or populations, greatly improving the accuracy of analysis. Molecular markers have advantages tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q2535/138
Inventor 董沁白小明孙吉雄方强恩吕优伟雷娅伟王婷赵春旭贺佳圆
Owner GANSU AGRI UNIV
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