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Method for improving resistance of hybrid rice restoring line

A technology of restorer and hybrid rice, applied in plant genetic improvement, botany equipment and methods, applications, etc., can solve the problems of no tracked introduction of resistance gene fragments, impossibility to improve the resistance of hybrid rice restorer lines, etc.

Inactive Publication Date: 2003-02-19
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, they neither tracked the size of the introduced resistance gene segment nor performed selection on the genetic background outside the resistance gene segment, so they could not be used for resistance improvement of hybrid rice restorer lines

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0029] Example 1: Improvement of Bacterial Blight Resistance of Hybrid Rice Restorer Line Minghui 63

[0030] a. Taking the line IRBB21 containing the broad-spectrum and high-resistance gene Xa21 as the donor parent, and the most widely used hybrid rice restorer line Minghui 63 in my country as the recurrent parent, the two are crossed to obtain F 1 Then return to the reincarnation parent to get BC 1 F 1 ;

[0031] b. To BC 1 F 1 Perform PCR analysis on individual plants, select those with Xa21 gene for AFLP analysis, select the single plant with the most genetic background of recurrent parents and backcross with recurrent parents to obtain BC 2 F 1 ;

[0032] c. to BC 2 F 1Perform PCR analysis on individual plants, select the Xa21 gene and exchange with the molecular marker AB9 on the side of the gene for AFLP analysis, select the single plant with the most recurrent parent genetic background and backcross with the recurrent parent to obtain BC 3 F 1 ;

[0033] d. To BC 3 F 1 The...

example 2

[0035] Example 2: Hybrid production method of improved Shanyou 63

[0036] The improved Minghui 63 and the sterile line Zhenshan 97A were planted under the conditions of good natural barrier isolation (other rice materials that met without flowering within a range of 100 meters around), and the row ratio of the improved Minghui 63 and Zhenshan 97A The ratio is 1:8, and the row spacing is 16×26cm. After the seeds are mature, the seeds are harvested on Zhenshan 97A, which is the improved Shanyou 63.

example 3

[0037] Example 3: Identification results of multi-bacteria inoculation of Minghui 63 and its improved type, Shanyou 63 and its improved type

[0038] In the summer of 1998, 14 bacterial blight strains that were pathogenic to Minghui 63 from major rice regions in my country, the Philippines, Japan and other countries were used in the hospital nursery of Huazhong Agricultural University in Wuhan to test Minghui 63 and its improved type, Shantou You 63 and its modified version have undergone multi-bacterial inoculation identification (see Table 1 for details). The results showed that the modified Minghui 63 and the modified Shanyou 63 derived from its combination showed disease resistance to all tested strains, reaching the same resistance level as the original resistant parent IRBB21, but compared with the original Minghui 63 and Shanyou 63. Compared with You 63, the resistance was significantly enhanced, and the expected goal was fully achieved.

[0039] Table 1. Multi-bacteria ino...

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PUM

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Abstract

The present invention belongs to the field of breeding new plant variety, and features that through the breeding process including one cross breeding, three backcrosses and one selfing, the destination gene is introduced into hybrid rice restoring line to be improved. By means of the detection of polymerase chain reaction and the selection of molecular marker gene co separated from and / or closely interlocked with the destination gene, the transferring fragment containing the destination gene is made to be as small as possible. By applying augmented fragment length polymorphism label in selecting genetic background, the improved restoring line has the same genome the same as original restoring line except for the destination gene fragment.

Description

Technical field: [0001] The invention belongs to a method for breeding new plant varieties, and specifically relates to a method for improving the resistance of hybrid rice restorer lines. Its international patent classification number is A01H1 / 00. Background technique: [0002] Since the realization of the three-line hybrid rice matching in 1973, my country has cultivated many excellent hybrid rice restorer line parents and formulated many strong hybrid rice combinations, which have made outstanding contributions to my country's food production. However, most of the restorer lines currently used have poor resistance, which severely restricts the further popularization and application of hybrid rice in my country. Backcross breeding has long been an effective way to improve existing varieties with good comprehensive traits but poor individual traits, especially a common method to improve the resistance of existing varieties. Usually the variety to be improved is used as the recur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/00
Inventor 陈升徐才国林兴华张启发
Owner HUAZHONG AGRI UNIV
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