Molecular marker breeding technology of corn and solanaceous vegetables

Abstract

The invention relates to a molecular marker seed-breeding technology of corn and solanaceous vegetables. The technology is mainly characterized by implementing researches of technologies, such as extraction and purification of DNA of crops, PCR (Polymerase Chain Reaction) and characteristic primer screening, RAPD (Random Amplified Polymorphic DNA) marking, SSR (sympathetic skin response) marking, AFLP (Amplified Fragment Length Polymorphism) marking, molecular marker-assisted selection of disease-resistance breeding materials, separation and DNA sequencing of target genes and the like aiming at crops, such as corn, tomato, eggplant, chili and the like.

Description

【Technical field】 [0001] The invention relates to a molecular marker breeding technology for corn and solanaceous vegetables, especially molecular marker-assisted selection breeding, which is an important part of biotechnology and has been widely used at home and abroad. The development of DNA polymerase chain reaction (PCR) has also made it possible to directly amplify DNA polymorphisms, and on this basis, various new molecular markers have been produced, such as RAPD markers, SSR markers, and AFLP markers. 【Background technique】 [0002] Due to the genetic polymorphism of DNA molecules in plants, there are a large number of non-coding DNA sequences in the genome, and their variation is not or less restricted by natural selection. Therefore, the application of molecular marker technology can be organically combined with traditional breeding techniques Combined, the accuracy and purpose of selection will be greatly enhanced, the selection efficiency of breeding materials wil...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above-mentioned technical problems, the technical solution adopted in the present invention is: the application of molecular markers in solanaceous vegetables to carry out assisted breeding research is relatively common, and the cDNA maps of the main shapes of these vegetables have been successfully drawn, and SAVR tomatoes have been bred, which overcomes the conventional The breeding method has shortcomings such as long cycle, heavy workload, low efficiency, and poor predictability. Through the application of molecular marker technology, the rapid and full use of pest-resistant materials can be achieved.