Researching method of dominant male genic male sterile material of hulless oat
A male nuclear and nuclear sterility technology, which is applied in the research field of naked oat dominant male sterile materials, can solve the problem of not finding dominant male sterile materials and the like
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[0050] The materials used in the embodiment of the present invention are divided into two categories, one is: female parental sterility material, and the other is: male parental material for testing and mating.
[0051] 1. Obtaining maternal sterility materials. In 1996, it was discovered and obtained in the naked oats experimental field of the former Bashang Agricultural Science Institute of Zhangjiakou City. The process is as follows: In 1994, two transgenic substances (Pucl8 and Gus) were introduced from the Institute of Botany, Chinese Academy of Sciences, and then transferred to the naked oat variety "Pin No. 5" as two salt-alkali resistance genes. The method is as follows: use a sharp blade to cut off the stigma of naked oat embryos that have been pollinated for 4 hours, drop 5 μl of DNA, and make 500 flowers for each treatment. There are 2 treatments in total, namely P5P and P5G. The seeds harvested in autumn are 95 and 115 respectively. In 1995, it was planted in the...
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