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Molecular markers closely linked to dwarf genes and their applications

A molecular marker and gene technology, applied in the field of crop genetics and breeding, can solve the problems of maize dense planting and lodging resistance

Active Publication Date: 2021-04-23
HENAN ACAD OF AGRI SCI INST OF GRAIN CROPS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention proposes a molecular marker closely linked with the dwarf gene and its application, which solves the technical problem of using SSR to quickly screen high-quality corn planting resources. Among them, umc1383 and umc2241 are closely linked with the dwarf gene in Zheng 58d. The method of molecular marker-assisted selection can introduce the dwarf gene resources of Zheng 58d into excellent backbone lines, create new dwarf germplasm resources, and apply them to dwarf breeding, which can solve technical problems such as close planting and lodging resistance in maize production

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  • Molecular markers closely linked to dwarf genes and their applications
  • Molecular markers closely linked to dwarf genes and their applications
  • Molecular markers closely linked to dwarf genes and their applications

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Experimental program
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Effect test

Embodiment 1

[0040] Acquisition of SSR molecular markers:

[0041] 1. Analysis of the genetic effect of the dwarf gene

[0042] Take dwarf Zheng 58d ( figure 1) were reciprocally crossed with Zheng 58 and B73, and it was found that the F1 phenotypes were all normal plants, indicating that the mutation belonged to a gene mutation in the nucleus and was a recessive gene; the corresponding BC1 and F2 segregation populations were constructed, and 258 BC1 strains The plant heights of the segregation population and 326 F2 segregation populations were investigated, and it was found that the proportions of normal plants and dwarf plants in the BC1 and F2 segregation populations were 136:122 and 252:74, respectively, and the chi-square test was completely consistent with 3 The segregation ratio of :1 or 1:1 indicated that Zheng 58d was controlled by the recessive monogenic gene (Table 1).

[0043] Table 1 Genetic analysis of dwarf mutant genes

[0044]

[0045] 2. Preliminary mapping of Zheng...

Embodiment 2

[0048] The application of molecular marker of the present invention in corn breeding, concrete steps:

[0049] In Hainan in the winter of 2013, the dwarf inbred line "Zheng 58d" was used as the donor, and the backbone self-selected lines Zheng 63, Zheng 36, Zheng 754, and Zheng P6 were used as the recipients, and the dwarf gene was introduced into the recipients through hybridization , get F1;

[0050] F1 plants were planted in Zhengzhou in the summer of 2014, and the pollen of Zheng 63, Zheng 36, Zheng 754, and Zheng P6 was used to backcross pollinate the F1 plants, and the BC1 population corresponding to Zheng 63, Zheng 36, Zheng 754, and Zheng P6 (containing AA and Aa two genotypes);

[0051] Use the "alkali boiling method" to quickly extract DNA: cut a small part of the endosperm and put it into a 96-well PCR plate, add 100ul of 0.1M NaOH, cover the PCR plate and heat bath at 100°C for 10 minutes on the PCR amplification instrument ; Take out and add an equal volume of 1...

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Abstract

The invention belongs to the field of crop genetics and breeding, in particular to molecular markers closely linked with dwarf genes and applications thereof. The molecular markers are umc1383 and umc2241, wherein the sequence of umc1383 is shown in SEQ ID No.1, and the sequence of umc2241 is shown in SEQ ID No.2. In the present invention, through the genetic analysis of a corn dwarf mutant (Zheng 58d) induced by EMS, it is found that the phenotype of the dwarf mutant is controlled by a recessive single gene. , heterozygous and homozygous pools were screened for polymorphisms, and the target gene was located on the first chromosome; two SSR molecular markers umc1383 and umc2241 closely linked to the target gene were screened out; , and rust resistance, excellent comprehensive properties.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, in particular to molecular markers closely linked with dwarf genes and applications thereof. Background technique [0002] Breeding practice has proved that every breakthrough in breeding depends on the discovery and utilization of favorable genes. The first revolution in rice breeding occurred in the 1960s. Based on the discovery of the dwarf gene sd and its application in dwarf breeding, the yield, resistance and variety of rice have been greatly improved. Corn is an important food crop and an important source of animal feed and industrial raw materials. Zhengdan 958 is the variety with the largest planting area in my country. It has ranked first in the planting area for 15 consecutive years, and has created great economic, social and ecological benefits. In view of its high yield, high density, stable yield, wide adaptability, multi-resistance and suitable for full-process mechanizat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 鲁晓民曹丽茹宋迎辉王振华魏良明张新张前进魏昕郭书磊郭金生
Owner HENAN ACAD OF AGRI SCI INST OF GRAIN CROPS
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