Method for quickly and accurately identifying brewing barley variety by utilizing DNA (Deoxyribose Nucleic Acid)-AFLP (Amplified Fragment Length Polymorphism)

Abstract

The invention discloses a method for identifying different brewing barley genetic resources at home and abroad by combining a DNA (Deoxyribose Nucleic Acid)-AFLP (Amplified Fragment Length Polymorphism) molecular marker with biological analyzing software. The method is characterized by comprising the following steps of: establishing a plant genome DNA extracting method suitable for AFLP and a barely germ plasma analyzing AFLP system, and carrying out electrophoresis and silver staining onto an amplified product on 6% of denatured polyacrylamide gel; establishing an AFLP fingerprint spectrum of 27 brewing barely varieties at home and abroad by utilizing three pairs of EcoRI / MseI primer combinations (E8 / M1, E8 / M3, E8 / M5) AFLP. By carrying out statistic analysis through banding pattern analyzing software POPGEN, NTSYS 3.0 and STATISTICA / w5.0, the unique banding patterns of 27 materials can be mutually distinguished from other varieties by utilizing 10 specific sites according to electrophoretogram, so that foundation is laid up for furthermore malt production and beer process.

Description

technical field [0001] The invention belongs to the field of identifying different brewing barley varieties by using molecular biology experiment technology, uses molecular biology technology DNA-AFLP combined with biological analysis software to quickly and accurately identify beer brewing barley varieties, improves the accuracy of malting malting, and can be widely used in beer production In the field of medium malt malting, it plays an important role in selecting different varieties of malt for beer production with different tastes and qualities, and improving the quality and taste of beer. At the same time, it can also improve the purity of malt export, reduce international trade disputes and achieve a certain protective effect. Background technique [0002] Barley is the main raw material of the beer industry, and its quality and quality will directly affect the quality of beer. my country's beer industry has developed rapidly since the reform and opening up. In recent...

AI Technical Summary

Problems solved by technology

Wenzl et al. (2004) applied this technology to the polymorphism research of barley varieties, and Druka (2001) applied cDNA-AFLP technology to the construction of barley fingerprints, but the operation process is relatively cumbersome and the actual use is poor

In 2005, Hou Yongcui et al. used RAPD markers to study the genetic diversity of barley varieties, but there is no report on the use of DNA-AFLP in the identification of brewing barley varieties in China.

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