Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for identifying Qianshan No.1, new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. by utilizing AFLP (amplified fragment length polymorphism)-CE (capillary electrophoresis) technique

A technology of capillary electrophoresis and scutellaria, applied in biochemical equipment and methods, measurement/inspection of microorganisms, material analysis through electromagnetic means, etc., to achieve the effects of easy standardized analysis, improved reliability, and reduced errors

Inactive Publication Date: 2014-01-01
红河千山生物工程有限公司
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, no AFLP molecular marker technology has been used for molecular identification of breviscapine, nor has AFLP-capillary electrophoresis technology been used for molecular identification of the new species of breviscapine Qianshan No. 1

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying Qianshan No.1, new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. by utilizing AFLP (amplified fragment length polymorphism)-CE (capillary electrophoresis) technique
  • Method for identifying Qianshan No.1, new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. by utilizing AFLP (amplified fragment length polymorphism)-CE (capillary electrophoresis) technique
  • Method for identifying Qianshan No.1, new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. by utilizing AFLP (amplified fragment length polymorphism)-CE (capillary electrophoresis) technique

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0038] Main experimental instruments:

[0039] CEQ8000 capillary electrophoresis system (Beckman Coulter); desktop refrigerated centrifuge (Beckman Coulter); PCR instrument (MJ Research PTC200-well thermal cycler); ultraviolet spectrophotometer (UV-2550, SHIMADZU company), gel imaging system.

[0040] The method of the present invention adopts conventional AFLP-capillary electrophoresis technology except for the following special instructions. Various reagents used in the following examples were purchased from Shanghai Sangong Hebao Biological Co., Ltd.

[0041] The molecular identification method of Qianshan No. 1, a new variety of Erigeron breviscapus according to the present invention, is carried out according to the following steps:

[0042] (1) Collection of samples: A total of 5 samples were collected from Luxi County (Qianshan No. 1), Honghe Hani and Yi Autonomous Prefecture, the main producing area of ​​Erigeron breviscapus in Yunnan Province, Dali City in Yunnan Prov...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for identifying Qianshan No.1, a new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. by utilizing AFLP (amplified fragment length polymorphism)-CE (capillary electrophoresis) technique, belonging to the technical field of molecular markers. The method comprises the steps of extraction of sample DNA, double digestion of DNA samples and linking of double digestion products, pre-amplification of linking products, selective amplification, AFLP-CE detection and result analysis and identification. By adopting the method, an AFLP-CE separation diagram of Qianshan No.1, a new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. is successfully established, whether the samples are Qianshan No.1, a new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz. can be easily and accurately identified and scientific bases are provided for protecting Qianshan No.1, a new variety of Erigeron Breviscapus (Vant.)Hand.-Mazz..

Description

technical field [0001] The invention relates to the technical field of molecular markers, in particular to random amplified fragment length polymorphism (AFLP)-capillary electrophoresis technology, and molecular identification of a new species of breviscapine, Qianshan No. 1. Background technique [0002] Breviscapus [Erigeron breviscapus (Vant.) Hand.-Mazz.] is a perennial plant of the genus Asteraceae. It is a good natural medicine for treating cardiovascular and cerebrovascular diseases. Its total flavonoids, breviscapus, and caffeoylquinic acid There are 4 types of injections developed with similar compounds as active ingredients, and it is one of the most potential medicinal plants in Yunnan Province. [0003] Scutellarin is one of the main functional components of scutellarin. The content of scutellarin determines the curative effect of medicinal materials, and is also an important criterion for identifying the quality of medicinal materials. Due to the increasing so...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N27/447
CPCC12Q1/686C12Q2535/138C12Q2565/125
Inventor 张薇杨生超张广辉杨建文吴道聪龙光强岳艳玲
Owner 红河千山生物工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com