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Method for detecting rose cockscomb character

A technology for roses and traits, applied in biochemical equipment and methods, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problems of inability to accurately distinguish dominant homozygous and heterozygous individuals, low breeding efficiency, etc. Achieve the effects of improving breeding and conservation efficiency, speeding up the breeding process, and high accuracy

Active Publication Date: 2011-05-04
李宁 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Compared with the single crown phenotype, the rose crown phenotype is a dominant trait controlled by a single point. Therefore, in the process of conservation and breeding, it is impossible to accurately distinguish dominant homozygous and heterozygous individuals according to the phenotype, resulting in the efficiency of breeding. low

Method used

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  • Method for detecting rose cockscomb character
  • Method for detecting rose cockscomb character
  • Method for detecting rose cockscomb character

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, establishment of PCR detection method and determination of polymorphic sites

[0019] 1. Genotype analysis

[0020] 1. Experimental materials: The CAU resource family is an experimental group established by the research group of Professor Li Ning of China Agricultural University in 1998 to study the location of QTLs that affect important economic traits of chickens and to find the main gene for cloning. The parents are French star broilers and Taihe silk feathers Silkie. In this experiment, 278 individuals of the F0, F1 and F2 generations of 4 CAU resource families were selected.

[0021] 2. Genomic DNA extraction

[0022] Blood was collected from the wing vein of the 12-week-old chicken, lysed after anticoagulant treatment, digested with proteinase K, extracted with phenolic form, dissolved in TE and stored at -20°C.

[0023] 3. PCR amplification

[0024] Using the genomic DNA extracted in step 2 as a template, the CCDC108 gene was amplified by PCR wi...

Embodiment 2

[0042] Embodiment 2, the application of molecular marker

[0043] 6 breeds with rose crown phenotype (all obtained from Poultry Research of Chinese Academy of Agricultural Sciences , Beijing Poultry Breeding Company) and non-rosecrown phenotypes (including non-rosecrown phenotypes such as single crown and bean crown) were measured, and the specific varieties are shown in Table 2 and Table 3.

[0044] 1. Genomic DNA extraction

[0045] Blood was collected from the wing vein of the 12-week-old chicken, lysed after anticoagulant treatment, digested with proteinase K, extracted with phenolic form, dissolved in TE and stored at -20°C.

[0046] 2. PCR amplification

[0047] Using the genomic DNA extracted in step 1 as a template, the CCDC108 gene was amplified by PCR with primers.

[0048] RosecombF1: 5'-GCCCCTTCCTGAAGCAATA-3' (as shown in SEQ ID NO.1 Show);

[0049] RosecombF2: 5'-GACTCCTTCCATCGCTCAGA-3' (as shown in SEQ ID NO.2 Show);

[0050] RosecombR: 5'-CCTTGTTCCCAGC...

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Abstract

The invention provides a primer pair consisting of nucleotide sequences shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The primer pair can be applied to the detection of a rose cockscomb character. The invention also provides a method for detecting the rose cockscomb character by using the primer pair. By the method, an obvious correlation property of amplified fragment length polymorphism and the rose cockscomb character in an intron 3 of a coiled-coil domain containing 108 (CCDC108) gene of a chicken is discovered and an amplified fragment can be used as a genetic marker of the chicken,so a molecular biological method is used for auxiliary selection of the marker.

Description

technical field [0001] The invention relates to a method for molecular marker detection of cockscomb traits by means of molecular biology. Background technique [0002] With the development of animal husbandry, appearance characteristics play an important role in chicken breeding and conservation, and molecular markers also play an important role in breeding and conservation. However, there are still few studies on molecular markers controlling appearance traits, and only a small number of appearance traits can be assisted by molecular markers for selective breeding. [0003] Cockscombs are derived from the skin and are located on the head. Different breeds have different comb shapes. The comb shape is one of the characteristics of the breed, including single comb, rose comb, bean comb, meat pad comb, strawberry comb, cup-shaped comb, feather comb, etc. 7 The color of the crown is mostly red, and the color is bright, delicate, plump and moist, which are the symptoms of heal...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 胡晓湘冯春刚刘冉冉顾晓荣王彦强李宁
Owner 李宁
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