Molecular marker of Pyrus communis L. fruit titratable acid content

A technology of molecular markers and acid titration, applied in the field of molecular genetics and breeding, can solve problems such as lack of development, achieve the effects of improving accuracy, improving accuracy and efficiency, and speeding up progress

Inactive Publication Date: 2011-07-13
NANJING AGRICULTURAL UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on QTL mapping of quantitative traits of pear is still in its infancy. The existing researches are mainly on some diseases or growth traits. The research on QTL mapping and molecular markers of titratable acid, one of the important quality traits, has not yet been carried out.

Method used

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  • Molecular marker of Pyrus communis L. fruit titratable acid content
  • Molecular marker of Pyrus communis L. fruit titratable acid content
  • Molecular marker of Pyrus communis L. fruit titratable acid content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] a) Using the hybrid combination of two pear varieties 'August Red' and 'Dangshansuli' in my country, 97 F 1 group.

[0024] b) Analyze the two parents and their F 1 Population, statistical analysis of genetic types of polymorphic sites between parents in offspring populations. A total of 732 polymorphic marker loci, including 23 SSRs, S gene loci, 226 SRAPs, and 482 AFLPs, were finally obtained, and then linkage analysis was performed on them using Jionmap3.0 mapping software to construct a table containing Genetic linkage map of 240 polymorphic markers in 'August Red' pear.

[0025] Using SSR, SRAP, and AFLP molecular marker methods, the 'August Red' and 'Dangshan Suli' and their offspring were analyzed, and the genetic types of the polymorphic sites between the parents were statistically analyzed in the offspring population.

[0026] For the experimental procedure of SSR labeling, refer to the method of Yomamoto T. et al. (Euphytica, 2002, 124:129-137); for the exp...

Embodiment 2

[0046] Using the screened molecular markers linked to the main QTL for titratable acid content in fruit of ‘Augusthong’, 97 strains of F in ‘Augusthong’ and ‘Dangshansuli’ 1 Preliminary testing was carried out on the hybrid progenies to test the practical value of this method in molecular marker-assisted selection of titratable acid content in pear fruit. Use the genomic DNA of the 97 offspring and AFLP selective amplification primer combination E-AAC / M-CAG to carry out PCR reaction, after electrophoresis according to EAACMCAG-850m * Whether there is a corresponding marker to determine whether there is a corresponding marker. If there is a marker, it means that the fruit titratable acid content of the plant is high, and if it does not exist, it means low. The results are verified.

[0047] The results showed that the 97 F 1 Among the hybrid offspring, a total of 63 strains showed EAACMCAG-850m in DNA amplification results * Among these 64 strains, there are 35 strains whose...

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Abstract

The invention provides a molecular marker of the Pyrus communis L. fruit titratable acid content, belonging to the genetic breeding field. Sequence related amplified polymorphism (SRAP), simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) are utilized to construct the genetic linkage maps of Pyrus communis L., the phenotypic identification of the fruit titratable acid content is combined, the interval mapping method is adopted to analyze the colony, a main effect quantitative trait locus (QTL) exists in the third linkage group of Pyrus communis L. through detection, and the characteristic that the titratable acid content is 49.3% can be explained. The marker EAACMCAG-850m* is most near the main effect QTL Pta-1, and the distance between the marker and the locus is 2cm. The obtained molecular marker of titratable acid main effect QTL has important theoretical and practical significances for accelerating the genetic improvement process of Pyrus L. cultivars and increasing the breeding selection efficiency.

Description

1. Technical field [0001] The invention provides a molecular marker for titratable acid content of pear fruit, belongs to the field of molecular genetic breeding, and can be used for early molecular assisted selection of titratable acid content traits of pear fruit to improve breeding efficiency. 2. Background technology [0002] Pear (Pyrus L.) is native to my country and is the third largest fruit tree species in my country. Pears are deeply loved by consumers because of their high nutritional value, sweetness and juiciness. Titratable acid is one of the important factors affecting the quality of pear fruit. As a unique component of pear fruit, it not only affects the quality of fresh food, but also affects the quality of processing. Therefore, reducing the content of titratable acid in pear fruit is very important to improve the quality of pear fruit. Breeding varieties with less titratable acid content has become one of the important goals of pear breeding. [0003] S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 张绍铃李秀根张瑞萍吴俊吴华清陶书田齐开杰杨健王龙王苏珂
Owner NANJING AGRICULTURAL UNIVERSITY
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