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Molecular marker of line with genic sterile recessive epistatic interaction in brassica napus and application

A technology of recessive epistatic interaction and Brassica napus, which is applied in the field of marker-assisted breeding of temporary protection lines, can solve the problem of difficulty in selection and breeding of temporary protection lines for nuclear male sterility, and achieve the effect of facilitating the utilization of heterosis.

Inactive Publication Date: 2010-11-10
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because it involves a pair of recessive sterile genes and a pair of multiple allelic epistatic genes, it is difficult to select and breed nuclear male sterile temporary insurance lines

Method used

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  • Molecular marker of line with genic sterile recessive epistatic interaction in brassica napus and application
  • Molecular marker of line with genic sterile recessive epistatic interaction in brassica napus and application
  • Molecular marker of line with genic sterile recessive epistatic interaction in brassica napus and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1: Acquisition of BnMs3, BnMs4 and BnRf-specific molecular markers related to recessive epistatic genic sterility in Brassica napus 1. Construction of three near-isogenic populations 7-7365AB, 7-736512AB and 7-7365AC:

[0053] The basic materials used are Brassica napus recessive male sterile homozygous two-type line 7-7365AB (Bnms3ms3RfRf / BnMs3ms3RfRf) (Huang et al, 2007), the new Brassica napus 7-749 (Bnms3ms3Ms4Ms4) (Huang Zhen, Ph.D. Paper, 2008, http: / / www.cnki.net / ) and its temporary insurance line 7-7365C (Bnms3ms3rfrf) (Xiao et al, 2008), three near isogenic lines 7-7365AB ( Huang et al, 2007), 7-736512AB (Huang Zhen, doctoral dissertation, 2008, China National Knowledge Network, http: / / www.cnki.net / ) and 7-7365AC) (Xiao et al, 2008), respectively for Screening of molecular markers closely linked to three genes, BnMs3, BnMs4 and BnRf.

[0054] Construction of the near-isogenic line 7-7365AB: use the sterile line (Bnms3ms3RfRf) and the fertile line (BnMs...

Embodiment 2

[0113] Example 2: Breeding technology of excellent temporary protection line and improved temporary protection line homologous to the improved sterile line by using the co-dominant molecular markers of recessive nuclear epistatic sterility and BnMs3 / BnMs4 / BnRf linkage in Brassica napus The route is as follows ( Figure 9 ):

[0114] 1. Use the sterile single plant 7-7365A (Bnms3ms3RfRf) in the recessive epistatic interaction male sterile homozygous two-type line to cross with the temporary insurance line 7-7365C (Bnms3ms3rfrf) to obtain a fully sterile population, and divide it named S 1 (Bnms3ms3Rfrf) generation.

[0115] 2. Use the obtained S 1 A segregation population was obtained by crossing the fertile individual plant 7-7365B(BnMs3ms3RfRf) in the homozygous bitype line, which we named as S 2 generation.

[0116] 3. S 2 Four genotypes (BnMs3ms3Rfrf, BnMs3ms3RfRf, Bnms3ms3Rfrf, and Bnms3ms3RfRf) were included in the generation, and individuals with the target genotyp...

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Abstract

The invention belongs to a preparation field of a cole molecular marker, in particular to a molecular marker linked to recessive genic sterile gene in brassica napus and breeding of a temporary maintainer line homologous with a genic sterile line. The molecular maker is characterized by comprising the following steps of: establishing near-isogenic lines 7-7365AB, 7-736512AB and 7-7365AC of three genes BnMs3, BnMs4 and BnRf by using two types of male sterile lines 7-7365AB in the brassica napus, brassica napus 7-749 and temporary maintainer line materials 7-7365C as raw materials, and then sieving SSR (Simple Tandem Repeat) primers and AFLP (Amplified Fragment Length Polymorphism) primers and transforming a SCAR marker to obtain a codominant SSR molecular marker SR12384 linked to the BnMs3, dominant SCAR markers SM129, SM98, SM113 and codominant SCAR markers SM23 and H3 which are linked to the BnMs4, and a codominant SSR marker D1 linked to the BnRf. The invention can be used for auxiliary selection and breeding of recessive genic sterile markers and can also be used for fine mapping and cloning of sterile genes.

Description

technical field [0001] The invention belongs to the field of molecular marker preparation of recessive sterility gene in Brassica napus, and specifically relates to the preparation of molecular markers linked with fertility-related genes and the markers of temporary insurance lines homologous with male sterile lines in Brassica napus Auxiliary breeding and other aspects of the application. Background technique [0002] Male sterility is an effective way to utilize heterosis in rapeseed. So far, three-line hybrid seed production using cytoplasmic male sterility is still the most important way to utilize heterosis in Brassica napus. Cytoplasmic male sterility represented by pol CMS and Shan 2A CMS has been widely used in hybrid production. Nuclear male sterility is an important way to utilize heterosis in rapeseed, especially recessive nuclear male sterility shows some characteristics superior to cytoplasmic male sterility: complete and stable sterility; extensive recovery s...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 涂金星傅廷栋易斌黄镇肖麓俎峰夏胜前马朝芝沈金雄文静李兴华
Owner HUAZHONG AGRI UNIV
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