Method for screening manganese/cadmium absorption efficient ecological phytolacca americana

A screening method and an ecological technology, applied in the field of phytoremediation, can solve problems such as limiting the industrialization process of phytoremediation, and achieve the effects of low screening cost, convenient collection, and strong operability

Inactive Publication Date: 2017-12-15
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

At present, there is no unified international standard for the screening of phy...

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  • Method for screening manganese/cadmium absorption efficient ecological phytolacca americana
  • Method for screening manganese/cadmium absorption efficient ecological phytolacca americana
  • Method for screening manganese/cadmium absorption efficient ecological phytolacca americana

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Embodiment 1

[0030] A Screening Method for Efficient Manganese / Cadmium Absorption Ecological Pokeweed

[0031] Evaluation of Heavy Metal Pollution in Collection Areas of Wild Pokeweed Populations

[0032] Overview of collection areas: Sampling was carried out in 19 regions across the country from August to November 2014, and the heavy metal pollution in each region was investigated. The distribution of sampling points is as follows: 10 in Hunan, 3 in Guangxi, 3 in Jiangxi, and 1 in Zhejiang. and Jiangsu 2. During the sampling process, pokeweed leaves, seeds and corresponding surrounding soil were collected. (See Table 1 for details).

[0033] Sample collection method: In each sampling area, select 18 large pokeweed plants (over 2 years old), collect about 20 mature leaves each, and put them in a color-changing silica gel for sealed storage. Then collect the soil corresponding to the Pokeweed plant, use a shovel to select 5 points within a radius of 20cm around the root of Pokeweed to sa...

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Abstract

The invention relates to a plant restoration technology, and particularly discloses a method for screening manganese/cadmium absorption efficient ecological phytolacca americana. The method comprises the following steps: collecting in the wild leaves and seeds of phytolacca americana populations in a plurality of contaminated regions and uncontaminated regions in China and soil in the vicinity; detecting the manganese/cadmium content of the soil in the vicinity of the phytolacca americana populations in the regions, and selecting phytolacca americana populations in highly manganese contaminated, highly cadmium contaminated and uncontaminated regions respectively; performing AFLP (amplified fragment length polymorphism) molecular marker detection on the leaves of the selected phytolacca americana populations, and analyzing genetic structures thereof; selecting bred seedlings of the phytolacca americana populations to which different genetic differentiation occurs, culturing the seedlings for 50 days with manganese, cadmium and normal Hoagland solutions, and screening the manganese/cadmium absorption efficient ecological phytolacca americana by adopting the plant heights, fresh weights and leaf manganese/cadmium content of the phytolacca americana as indexes. According to the method, a manganese/cadmium absorption efficient ecological hyperaccumulator is used for treating manganese/cadmium-contaminated soil, and the heavy metal accumulation and restoration efficiency of the hyperaccumulator are improved.

Description

technical field [0001] The invention relates to phytoremediation technology, and relates to a screening method for heavy metal hyperaccumulation plants, in particular to a screening method for manganese / cadmium-absorbing high-efficiency molecular ecology pokeweed. Background technique [0002] With the development of society, the mining, smelting, processing and commercial manufacturing of heavy metals are increasing, and the pollution of heavy metals to soil is becoming more and more serious, which greatly endangers human health. The "National Soil Pollution Survey Report" shows that 19.4% of my country's cultivated land soil points exceed the standard, and heavy metals are the main pollutants in cultivated land soil. [0003] Manganese and cadmium are common heavy metals. Manganese is an essential trace element for plant growth, but excessive manganese can cause leaf wilting and necrosis, reduced leaf thickness, shortened internodes and reduced biomass. Cadmium is one of...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G06F19/12
CPCC12Q1/6827G16B5/00C12Q2535/138C12Q2521/301C12Q2525/191
Inventor 陈晨夏妍沈振国陈亚华陆敏
Owner NANJING AGRICULTURAL UNIVERSITY
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