Absolute quantification type digital nucleic acid analytic system based on efficient liquid drop microreactor

Abstract

The invention relates to inspection and quarantine systems for biomedicine, agriculture, food hygiene and the like and particularly relates to an absolute quantification type digital nucleic acid analytic system based on an efficient liquid drop microreactor. The absolute quantification type digital nucleic acid analytic system is mainly used for detecting the molecular structure of nucleic acid so as to analyze attributes of organisms and belongs to the fields of biological and medical detection. The absolute quantification type digital nucleic acid analytic system comprises a confocal fluorescence image collecting system, a micro-fluidic chip, a temperature circulating heating system, an electric loading platform, an automatic sample feeding device, a controller and a computer. The formation and PCR amplification of micro-droplets are finished by virtue of the same micro-fluidic chip, namely the micro-fluidic chip has a droplet formation function and a PCR amplification (microreactor) function. According to the absolute quantification type digital nucleic acid analytic system, the complexity of the absolute quantification type nucleic acid analysis process is reduced, the nucleic acid analysis process is finished in a one-button manner, and culture and bacteria-proliferating processes are omitted, so that the rapid detection is realized, the sensitivity of the absolute quantification type nucleic acid detection is greatly improved, meanwhile, the sample consumption of the absolute quantification type nucleic acid analysis is substantially reduced, and the high throughput detection is realized.

Description

technical field [0001] The invention relates to inspection and quarantine systems for biomedicine, agriculture, food hygiene, etc., specifically an absolute quantitative digital nucleic acid analysis system based on a high-efficiency droplet microreactor, which is mainly used to detect the molecular structure of nucleic acid, thereby analyzing the attributes of organisms , belonging to the field of biological and medical testing. Background technique [0002] Nucleic acid is a high molecular compound in living organisms. Including deoxyribonucleic acid (deoxyribonucleic acid, DNA) and ribonucleic acid (ribonucleic acid, RNA) two categories. Nucleic acid is not only the basic genetic material, but also plays an important role in the biosynthesis of proteins, so it plays a decisive role in a series of major life phenomena such as growth, heredity, and variation. The advantage of nucleic acid analysis technology lies in its high sensitivity and specificity. The detection and ...

AI Technical Summary

Problems solved by technology

The sample detection of the QX200 droplet digital PCR requires three instruments to be completed in three steps. First, droplets are generated in the droplet generation instrument, and the droplets are collected by PCR tubes and transferred to the PCR amplification instrument for amplification. After the amplification is completed, the amplification product is transferred to the data reading analyzer for reading the result. The analysis process is cumbersome, complicated, and the cycle is long, which is not suitable for rapid nucleic acid analysis and detection.

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