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Protein characteristic spectrum of active tuberculosis in children and method for creating protein characteristic spectrum

A construction method, a mobile technology, applied in the field of proteomics, can solve the problem of low sensitivity

Inactive Publication Date: 2015-07-22
BEIJING CHILDRENS HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some studies believe that the sensitivity of this test is very low even in children with positive bacterial cultures, and it is not suitable for use as a routine diagnostic method in children. Therefore, to explore new diagnostic methods suitable for children, It is imperative to achieve the goal of early diagnosis and early treatment

Method used

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  • Protein characteristic spectrum of active tuberculosis in children and method for creating protein characteristic spectrum
  • Protein characteristic spectrum of active tuberculosis in children and method for creating protein characteristic spectrum
  • Protein characteristic spectrum of active tuberculosis in children and method for creating protein characteristic spectrum

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Experimental program
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Effect test

Embodiment 1

[0047] Embodiment 1, the establishment of active tuberculosis protein profile in children

[0048] 1. Samples and instruments

[0049] 39 cases were selected from children's plasma samples aged 3 months to 16 years, of which 19 cases were plasma samples from children with active tuberculosis, and the other 20 cases were plasma samples from healthy children. All children with active tuberculosis were confirmed by laboratory and clinical diagnosis reports . All plasma samples were drawn in the morning on an empty stomach, and the plasma was separated and stored in a -80 low-temperature refrigerator.

[0050] The experimental instruments were liquid chromatography-electrospray ionization tandem mass spectrometry (Q-Exactive Thermo Finnigan), electrophoresis instrument (Bio-rad, USA), MultiSkans microplate reader (Thermo, USA), albumin / IgG removal kit was purchased from German Merck company.

[0051] 2. Protein extraction

[0052]The ProteoExtract Albumin / IgG Removal Kit from ...

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Abstract

The invention discloses a protein characteristic spectrum of active tuberculosis in children and a method for creating the protein characteristic spectrum. The protein characteristic spectrum of the active tuberculosis in the children is obtained by comparing proteomics difference of an active tuberculosis group and a healthy control group. In addition, the invention further relates to the method for creating the protein characteristic spectrum of the active tuberculosis in the children, protein in blood plasma can be effectively identified and relatively quantified by utilizing a non-marked tandem mass spectrum technology of relative and absolute quantification, and a protein expression difference mass spectrum in the blood plasma of a patient suffering from the active tuberculosis in the children can be obtained by adopting the technology. A series of discovered proteins provide a foundation and resources for searching new more ideal markers; compared with a conventional blood plasma detection method, the method has relatively high sensitivity and specificity, and can be used for screening drugs for the active tuberculosis in the children; at the same time, a new way is provided for a mechanism for exploring occurrence and development of diseases.

Description

technical field [0001] The invention belongs to the field of proteomics, and relates to a protein signature spectrum of children with active tuberculosis and a construction method thereof. Background technique [0002] Tuberculosis is one of the most common infectious diseases in the world today caused by Mycobacterium tuberculosis (MTB) infection, and it is one of the important diseases currently causing death of children in developing countries. It is estimated that children with tuberculosis have accounted for 10%-15% of the total number of tuberculosis patients in the world, and this rate is higher in countries and regions with high incidence of tuberculosis such as Africa. As one of the countries with high incidence of tuberculosis in my country, the tuberculosis infection rate of children aged 0-14 is 9.0%, and the prevalence rate of active tuberculosis is 91.8 / 100,000. With the prevalence of drug-resistant Mycobacterium tuberculosis and the increase of HIV virus infe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 申阿东李洁琼孙琳徐放綦辉肖婧申晨焦伟伟郭雅洁王咏红佟月娟
Owner BEIJING CHILDRENS HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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