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Method for determining soybean seed vigor based on nuclear magnetic resonance technique

A soybean seed and nuclear magnetic resonance technology, applied in the field of analytical chemistry, can solve the problems of difficult to quickly achieve the expected purpose, time-consuming and laborious evaluation, long cycle and other problems, and achieve the effect of accurate measurement, reliable data and large contribution rate.

Active Publication Date: 2017-05-17
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The evaluation of anti-aging soybean varieties is mostly judged by testing the vigor of seeds. Conventional methods include seed germination experiments, physiological measurements, accelerated aging methods, electrical conductivity measurements, and biochemical measurements. These methods have the disadvantages of long periods and large amounts. , there are also unstable factors such as man-made interference to the final result, resulting in time-consuming and labor-intensive evaluation, especially for the identification of the vitality of rare test materials, it is difficult to quickly achieve the desired purpose, therefore, a fast and accurate method is urgently needed in practice. , a seed vigor detection method that is not easily disturbed by external uncertain factors

Method used

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  • Method for determining soybean seed vigor based on nuclear magnetic resonance technique
  • Method for determining soybean seed vigor based on nuclear magnetic resonance technique
  • Method for determining soybean seed vigor based on nuclear magnetic resonance technique

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] In this embodiment, soybean and black soybean seeds of different years are selected, and the soybean seed sample information is shown in Table 1.

[0066] Table 1 Material information of samples

[0067] material code source harvest year Seed coat color JP6-H24 Hokkaido, Japan 2012 black beans JP6-H25 Hokkaido, Japan 2013 black beans JP16-H24 Hokkaido, Japan 2012 soy JP16-H26 Hokkaido, Japan 2014 soy

[0068] First, the four sample materials were ground and pulverized with liquid nitrogen and freeze-dried for 24 hours using a freeze dryer, and then the obtained soybean powder was stored in a -80°C ultra-low temperature refrigerator for later use.

[0069] Secondly, the analysis steps of the freeze-dried powder of each sample are as follows:

[0070] 1) Accurately weigh 500.0 mg of the freeze-dried powder of the soybean sample, place it in a sample tube, add 1 mL of methanol aqueous solution with a volume conce...

Embodiment 2

[0086] The soybean samples to be tested in this example were selected as the soybean sample JP16-H24 and the black soybean sample JP6-H24 purchased in 2012 as experimental materials, and the soybean seed sample information is shown in Table 3.

[0087]Table 3 Material information of two different soybeans

[0088] material code source harvest year Seed coat color JP6-H24 Hokkaido, Japan 2012 black beans JP16-H24 Hokkaido, Japan 2012 soy

[0089] First, the above two sample materials were ground and pulverized with liquid nitrogen and freeze-dried for 24 hours using a freeze dryer, and then the obtained soybean powder was stored in an ultra-low temperature refrigerator at -80°C for later use.

[0090] Secondly, the analysis steps of the freeze-dried powder of each sample are as follows:

[0091] 1) Accurately weigh 50.0 mg of the freeze-dried powder of the soybean sample, place it in a sample tube, add 1 mL of methanol aqueous solution w...

Embodiment 3

[0102] The same soybean material JP16 purchased in 2012 and 2014 was selected as the experimental sample for the soybean samples to be tested in this example, and the soybean seed sample information is shown in Table 5.

[0103] Table 5 Material information of two different soybeans

[0104] material code source harvest year Seed coat color JP16-H24 Hokkaido, Japan 2012 soy JP16-H26 Hokkaido, Japan 2014 soy

[0105] First, the above two sample materials were ground and pulverized with liquid nitrogen and freeze-dried for 24 hours using a freeze dryer, and then the obtained soybean powder was stored in an ultra-low temperature refrigerator at -80°C for later use.

[0106] Secondly, the analysis steps of the freeze-dried powder of each sample are as follows:

[0107] 1) Accurately weigh 50.0 mg of the freeze-dried powder of the soybean sample, place it in a sample tube, add 1 mL of methanol aqueous solution with a volume concentration of ...

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Abstract

The invention discloses a method for determining soybean seed vigor based on a nuclear magnetic resonance technique. The method is characterized by comprising the steps of carrying out absolute quantification analysis on metabolite in soybean seed extract by using a nuclear magnetic resonance wave spectrum technique to obtain a metabolite attribution map, screening specific metabolite based on an established partial least square method multiple regression model, then determining the vigor level by using an absolute content value of the specific metabolite in the soybean seeds, and providing reference for determining the soybean seed vigor and screening anti-aging soybean seed resources. The method is simple and rapid to operate and reliable in result, and can provide reference for determining and screening high-quality soybean seed resources with anti-aging potential.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, and more specifically relates to a method for discriminating the vigor of soybean seeds through metabolomics technology based on nuclear magnetic resonance spectrum technology. Background technique [0002] During the storage process, seeds will be aged to varying degrees, which will directly lead to the reduction of seed vigor, inhibit the normal development of embryos and seedling growth, and cause a significant decline in plant production level and quality. Therefore, the vigor of seeds must be tested before agricultural production to ensure the use of high vigor seeds for sowing and reduce the production loss caused by seed inactivation. [0003] Take the breeding of anti-aging high-quality soybean varieties as an example: seeds play a pivotal role in biodiversity conservation, agricultural, forestry and animal husbandry production, and plant community reconstruction and successi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08
CPCG01N24/082
Inventor 刘江秦雯婷杨才琼胡宝予邓俊才张潇文张静杨文钰
Owner SICHUAN AGRI UNIV
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