6 results about "Hepatitis B virus DNA" patented technology

The invention discloses a detection system for simultaneously detecting HBV (Hepatitis B Virus) DNA (Deoxyribose Nucleic Acid) and HBV RNA (Ribonucleic Acid) based on CRISPR-cas12a / cas13a. According to the invention, a PCR primer is designed to amplify conserved intervals of different genotypes of HBV DNA, and a PCR primer with a T7 promoter is designed to amplify a conserved sequence of HBV RNA. In the same detection system, crRNA of cas12a / cas13a can specifically recognize amplification products of the crRNA respectively, HBV DNA and HBV RNA can be detected in a single tube at the same time, and finally visual detection is achieved through direct color development of dual fluorescence. According to the method, HBV DNA and HBV RNA can be efficiently, specifically and accurately detected, so that the clinical antiviral curative effect of the chronic hepatitis B patient is better monitored, medication is guided, and accurate treatment on the chronic hepatitis B patient is facilitated.

The present disclosure relates to a ribavirin derivative-embedded double-stranded siRNA analogue, a conjugate comprising the same, and salts and uses thereof. The double-stranded siRNA analogue, the conjugate comprising the same, and the salts thereof of the present disclosure can effectively inhibit multiple viral indicators such as hepatitis B virus DNA, pgRNA, S antigen, E antigen, etc., and provide an effective and feasible method for the treatment of hepatitis B.

The invention discloses a method and kit for detecting hepatitis B virus DNA by a self-assembled label-free CRISPR biosensor based on signal amplification, (1) performing RCA amplification on the HBV nucleic acid sample to be detected; (2) mixing the amplified product of step (1) with Cas12a, crRNA and biotin-labeled ssDNA, incubating the reaction and then heating to inactivate Cas12a; (3) adding S1 to the reaction solution treated by step (2) for incubation and hybridization, then adding S2 for incubation and hybridization, adding streptavidin magnetic ball for oscillation incubation after completion, separating the magnetic ball after incubation, adding fluorescent dye SYBR Green I for incubation, and using a fluorescence spectrophotometer to measure the fluorescence intensity after incubation. Due to the powerful signal output performance brought by multiple signal amplification, the designed method has the advantages of high sensitivity and wide linear range.

The present application relates to a kind of based on magnetic covalent organic framework material construction biological sensor method for detecting hepatitis B virus, the method includes Au@ZnFe2O4@COF Nanocomposite and intermediate product material: ZnFe2O4@COF.The outstanding creativity of the present application: 1.firstly using magnetic adsorption biological sensor detects HBV.2.design capture probe, signal probe and can specifically recognize the characteristic of hepatitis B virus DNA instead of using antibody to capture hepatitis B virus and utilize CuO laccase activity determination virus concentration sandwich type photochemical immunosensor.

Provided are a ribavirin derivative-embedded double-stranded siRNA analog, a conjugate comprising the same, and a salt and use thereof. The provided double-stranded siRNA analog, the conjugate comprising the same, and the salt thereof can effectively inhibit multiple viral indicators such as hepatitis B virus DNA, pgRNA, S antigen, E antigen, etc., and provide an effective and feasible method for the treatment of hepatitis B.

Provided herein are epigenetically modified DNA targeting systems, such as CRISPR-Cas / Guiding RNA (gRNA) systems, for transcriptional repression of Hepatitis B Virus (HBV) genes to promote cell phenotypes that lead to reduced HBV infection. In some embodiments, the epigenetically modified DNA targeting system binds to or targets a target site of at least one gene in a hepatitis B virus DNA sequence or a regulatory element thereof in a cell. In some aspects, the provided systems relate to transcriptional repression of one or more hepatitis B virus genes and / or regulatory elements thereof. In some aspects, also provided herein are methods and uses related to the provided compositions, for example, in repression of hepatitis B virus replication and expression associated with hepatitis B infection.