Method for fermenting mycelia from natural ophiocordyceps sinensis fruiting bodies

A technology of Cordyceps sinensis and fermentation bacteria, which is applied in the field of fermentation, and can solve the problems of unreachable component content, easy anger, and damage to human body functions.

Active Publication Date: 2015-04-01
四川藏宝虫草生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using Cordyceps sinensis asexually fermented mycelium, the content of ingredients cannot achieve a natural balance, and because of the high content of adenosine, it is particularly easy to become irritable, and long-term use can damage human body functions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for fermenting mycelium by natural Cordyceps fruiting bodies, carried out according to the following steps:

[0020] (1) Collect fresh wild Cordyceps fruiting bodies, transport them to the laboratory at 3°C, select Cordyceps with thick fruiting bodies that are about to erupt spores, wrap the underground part with plastic film, and use 8°C low-temperature water from the wrapped place along the fruiting bodies Rinse overnight;

[0021] (2) Cut off the water-rinsed part on the ultra-clean workbench, place it in a petri dish with alcohol, soak it for 1min, and then rinse it with sterile water;

[0022] (3) Place the sterilized fruiting body on a sterilized plate, put a piece of filter paper in the plate, after the filter paper absorbs the water of the fruiting body, cut the fruiting body with a scalpel, and grab the middle of the fruiting body with tweezers Mycelia, the sexual mycelium containing ascomycetes is clipped and placed in another clean Petri dish;

[0...

Embodiment 2

[0029] A method for fermenting mycelium by natural Cordyceps fruiting bodies, carried out according to the following steps:

[0030] (1) Collect fresh wild Cordyceps fruiting bodies, transport them to the laboratory at 0°C, select Cordyceps with thick fruiting bodies that are about to erupt spores, wrap the underground part with plastic film, and use 6°C low-temperature water from the wrapped place along the fruiting bodies Rinse overnight;

[0031] (2) Cut off the water-rinsed part on the ultra-clean workbench, place it in a petri dish with alcohol, soak it for 2 minutes, and then rinse it with sterile water;

[0032] (3) Place the sterilized fruiting body on a sterilized plate, put a piece of filter paper in the plate, after the filter paper absorbs the water of the fruiting body, cut the fruiting body with a scalpel, and grab the middle of the fruiting body with tweezers Mycelia, the sexual mycelium containing ascomycetes is clipped and placed in another clean Petri dish; ...

Embodiment 3

[0039] A method for fermenting mycelium by natural Cordyceps fruiting bodies, carried out according to the following steps:

[0040] (1) Collect fresh wild Cordyceps fruiting bodies, transport them to the laboratory at 3°C, select Cordyceps with strong fruiting bodies that are about to erupt spores, wrap the underground part with plastic film, and use 8°C low-temperature water from the wrapped place along the fruiting bodies Rinse overnight;

[0041] (2) Cut off the water-rinsed part on the ultra-clean workbench, place it in a petri dish with alcohol, soak it for 1min, and then rinse it with sterile water;

[0042] (3) Place the sterilized fruiting body on a sterilized flat plate, put a piece of filter paper in the flat plate, after the filter paper absorbs the water of the fruiting body, cut the fruiting body with a scalpel, and grab the middle of the fruiting body with tweezers Mycelia, the sexual mycelium containing ascomycetes is clipped and placed in another clean Petri ...

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PUM

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Abstract

The invention discloses a method for fermenting mycelia from natural ophiocordyceps sinensis fruiting bodies which belongs to the field of fermentation technology. The method first collects fresh wild ophiocordyceps sinensis fruiting bodies, lower-temperature water is used for washing along the fruiting bodies from the packaged parts overnight, the fruiting bodies are then soaked, and are washed with sterile water, mycelia are taken out, loaded into seed bottles with culture medium and cultured for 10 to 14 days, the ophiocordyceps sinensis mycelia in the seed bottles are put together to expand culture, finally, a fermentation tank is utilized for continuous culture, the ophiocordyceps sinensis mycelia are sampled to test the content of adenosine, cordycepin, polysaccharide and mycelia after cultured for 5 to 8 days, and if each index is not lower than the national standard and microscopic examination shows that the ophiocordyceps sinensis mycelia are not contaminated by microbes, the ophiocordyceps sinensis mycelia are harvested. Through the process of processing and fermenting the wild ophiocordyceps sinensis fruiting bodies, the method prepares the fermented mycelia, the content of adenosine, cordycepin, polysaccharide and mycelia in the prepared mycelia is high, moreover, the mycelia can be used for infecting swift moth larvae, and the method is one of the links of artificially cultivating ophiocordyceps sinensis.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a method for fermenting mycelium from natural cordyceps fruiting bodies. Background technique [0002] Cordyceps sinensis (Ophiocordyceps sinensis), also known as Cordyceps sinensis, is a complex of the subunit and larvae parasitized on the larvae of the green bat moth. Cordyceps sinensis is distributed in the alpine meadow area of ​​3000-5000m in my country's Tibet, Qinghai, Yunnan, Sichuan and Gansu provinces, and has extremely special habitat requirements. Under natural conditions, its life history is extremely complex, and it must go through relatively independent transitions between the conidia stage and the ascospore stage. Its host bat moth takes 5 to 6 years to complete a generation, and more than 85% of the time it lives underground , with generational overlap throughout the year. Cordyceps sinensis is warm in nature and sweet in taste, and has high me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCC12N1/14
Inventor 冯繻亿吴丽娟
Owner 四川藏宝虫草生物科技有限公司
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