Ganoderan high-yield strain RWHBW-1 and application thereof

A technology of high-yield strains and Ganoderma lucidum polysaccharides, applied in the field of agricultural microorganisms, can solve problems such as crop continuous cropping obstacles and crop quality decline, and achieve the effect of increasing yield and strong stability

Active Publication Date: 2019-06-28
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The use of fertilizers is an important means to increase agricultural production and income. However, the extensive use of chemical fertilizers has brought about

Method used

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  • Ganoderan high-yield strain RWHBW-1 and application thereof
  • Ganoderan high-yield strain RWHBW-1 and application thereof
  • Ganoderan high-yield strain RWHBW-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Obtaining Ganoderma lucidum strain RWHBW-1

[0037] (1) Mutagenesis method of Ganoderma lucidum strain

[0038] The Ganoderma lucidum strains collected in the wild were cultivated by PDA solid culture and PDB liquid culture to obtain a large number of mycelium, and the protoplasts of Ganoderma lucidum were obtained by enzymatic hydrolysis, and the protoplasts of Ganoderma lucidum were subjected to plasma discharge mutagenesis treatment, and after dilution, they were coated with A single colony was obtained from the cloth, and the treated strain was obtained after picking. The content of Ganoderma lucidum polysaccharide was detected on the strain to screen and obtain a high-yielding strain of Ganoderma lucidum polysaccharide.

[0039] (2) Screening of mutagenic ganoderma lucidum polysaccharide high-yielding strains

[0040] Using infrared spectroscopy-Ganoderma polysaccharide content detection method, the method in the literature (Ma Y, He H, Wu J, Wang C, Chao K, Huang...

Embodiment 2

[0043] Utilize grinding method to prepare the method for crude extract of Haematococcus pluvialis, comprise the following steps:

[0044] (1) When Haematococcus pluvialis grows to the stage of red cyst cells, the wet algae body is collected, the surface culture medium is sucked with filter paper, and weighed 2g; Haematococcus pluvialls in this example comes from the Wuhan Hydrobiological Research Institute of the Chinese Academy of Sciences Place;

[0045] (2) Grind in a mortar, add ethanol, grind while adding, until 5-10mL is dissolved, centrifuge at 3000rpm for 5-10min, remove the algae residue, keep the supernatant, and obtain the crude Haematococcus pluvialis after grinding. The extract is diluted 5-100 times for later use.

Embodiment 3

[0047] The method for preparing the crude extract of Haematococcus pluvialls by ultrasonic treatment, comprises the following steps:

[0048] (1) When Haematococcus pluvialis grows to the stage of red cyst cells, the wet algae body is collected, the surface culture medium is sucked with filter paper, and weighed 2g; Haematococcus pluvialls in this example comes from the Wuhan Hydrobiological Research Institute of the Chinese Academy of Sciences Place;

[0049] (2) After processing at 300W, 40KHz for 30min, centrifuge at 3000rpm for 5-10min to remove the algae residue, and take the supernatant to obtain the ground Haematococcus pluvialis crude extract, which is diluted 5-100 times for later use.

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Abstract

The invention discloses a ganoderan high-yield strain which is named as ganoderma lucidum strain RWHBW-1 with the strain preservation number of CCTCC NO. M2018499. The invention also discloses a special culture medium for producing ganoderan by fermenting the ganoderma lucidum strain RWHBW-1. Seeds of the ganoderma lucidum strain RWHBW-1 are inoculated in the special culture medium to promote growth and ganoderan synthesis of the ganoderma lucidum strain RWHBW-1. The ganoderma lucidum strain RWHBW-1 has the advantages that the ganoderma lucidum strain RWHBW-1 obtained by a mutagenesis method has the characteristics of strong stability and high yield of ganoderan; and the mycelial growth speed, the mycelium biomass and the ganoderan content under liquid fermentation conditions of the ganoderan strain RWHBW-1 are further improved by adding a microalgae crude extract. Compared with fruiting body planting, the ganoderan high-yield strain is not influenced by seasons and conditions and hasconsiderable advantages in production.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a ganoderma lucidum polysaccharide high-yield strain RWHBW-1 and its application. Background technique [0002] Ganoderma lucidum polysaccharide is one of the important medicinal ingredients of Ganoderma lucidum, a famous Chinese herbal medicine. Ganoderma lucidum polysaccharide has anti-tumor effect. Lymphocytes and T lymphocytes play a role. [0003] Among the 15 ganoderma strains reported by Li Xuquan, the mycelium polysaccharide content is the highest at 0.55g / L (Li Xuquan. Screening of high-yielding strains of ganoderma lucidum polysaccharides and research on polysaccharide physicochemical properties [D]. Shanghai Normal University, 2005.); Qing-HuaFang optimized the liquid fermentation conditions of Ganoderma lucidum. Under the optimal conditions, the intracellular polysaccharide content of Ganoderma lucidum was 1.06g / L (Fang QH, Zhong J J. Effect of in...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/04C12R1/645
Inventor 黄青马玉涵何华奇
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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