The invention discloses a medicated fresh dog meat soup base and its preparation method. The soup base is prepared by dog meat and Codonopsis pilosula, Chinese wolfberries, red dates, hawthorns, walnuts, Chinese chestnuts, dried orange peels, Acalypha australis, radix astragali, chrysanthemums, Clausena lansium leaves, ginkgo biloba, honeysuckle, morinda officinalis, Chinese angelica, and cloves, etc., additionally with salt, monosodium glutamate, chicken essence, sweet rice flour and rock sugar. The formula of the invention is characterized by scientific and reasonable collocation, abundant nutrition, delicious taste, and insatiable appetite by people, and has the functions of stabilizing five viscera, remedying sterility, tonifying yang, strengthening blood circulation, promoting gastrointestinal functioning, as well as enhancing kidney and bladder, etc. With the soup base of the invention, dog meat can be stewed with all the accessories together, or all the accessories can be stewed separately, then the obtained soup can be concentrated and packaged for sale. The preparation method provided in the invention is also simple and practical, so that usually consumers can make delicious and nutritional medicated fresh dog meat soups at home.