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Bacillus amyloliquefaciens and microbial inoculum, and production method and application of microbial inoculum

A technology of amylolytic spores and production methods, applied in the field of microorganisms, can solve the problems of strong pathogenicity, threat to the sustainable development of the alfalfa industry, yield and quality decline, etc., and achieve stable and long-lasting effects of biocontrol

Active Publication Date: 2017-01-25
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the single variety of alfalfa in my country and the increase in the number of years of use, alfalfa diseases, especially alfalfa root rot, are becoming more and more serious, which has seriously threatened the sustainable development of the alfalfa industry.
According to reports, the disease has seriously occurred in countries such as the United States, Canada, Australia, Russia, Japan, and Argentina, as well as domestic Xinjiang, Gansu, Qinghai, and Inner Mongolia. The annual output loss in the world is about 20%. up to 40%, which reduces the nitrogen fixation capacity of alfalfa, weakens the plants, and reduces the yield and quality
Among the pathogens of alfalfa root rot reported at home and abroad, there are 14 species of Fusarium, Fusarium chlamydoides F. chlamydosporum It is the main species with strong pathogenicity, and it is reported that the pathogen can also cause various plant diseases

Method used

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  • Bacillus amyloliquefaciens and microbial inoculum, and production method and application of microbial inoculum
  • Bacillus amyloliquefaciens and microbial inoculum, and production method and application of microbial inoculum
  • Bacillus amyloliquefaciens and microbial inoculum, and production method and application of microbial inoculum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] A microbial agent, the active ingredient of which is Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), the cell of CGMCC No.13047 strain, and the number of live bacteria of Bacillus amyloliquefaciens is 0.1×10 14 CFU / mL. The preparation method of microbial inoculum is to insert the Bacillus amyloliquefaciens strain into the substrate for fermentation, and the substrate is composed of the following materials: bran 55%, rice husk powder 20%, corn flour 15%, soybean meal 10%, maltose 10g / kg , Tryptone 20g / kg and MgSO 4 15g / kg. The incorporation amount of Bacillus amyloliquefaciens strain was 6% by weight, the ratio of material to water was 1:1, the fermentation time was 36 hours, and the fermentation temperature was 34°C. Microbial agents can be used to prepare pesticides or fertilizers.

Embodiment 2

[0101] A microbial agent, the active ingredient of which is Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), the cell of CGMCC No.13047 strain, and the number of viable bacteria of Bacillus amyloliquefaciens is 2.0×10 14 CFU / mL. The preparation method of the microbial agent is to insert the Bacillus amyloliquefaciens strain into the substrate for fermentation, and the substrate is composed of the following materials: bran 55%, rice husk powder 20%, corn flour 15%, soybean meal 10%, maltose 10g / kg, tryptone 20g / kg and MgSO 4 15g / kg. The incorporation amount of Bacillus amyloliquefaciens strain was 10% by weight, the ratio of material to water was 1:1, the fermentation time was 42 hours, and the fermentation temperature was 37°C. Microbial agents can be used to prepare pesticides or fertilizers.

Embodiment 3

[0103] A microbial agent whose active ingredient is Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), the cell of CGMCC No.13047 strain, and the number of live bacteria of Bacillus amyloliquefaciens is 1.84×10 14 CFU / mL. The preparation method of the microbial agent is to insert the Bacillus amyloliquefaciens strain into the substrate for fermentation, and the substrate is composed of the following materials: bran 55%, rice husk powder 20%, corn flour 15%, soybean meal 10%, maltose 10g / kg, tryptone 20g / kg and MgSO 4 15g / kg. The incorporation amount of Bacillus amyloliquefaciens strain was 14% by weight, the ratio of material to water was 1:1, the fermentation time was 48 hours, and the fermentation temperature was 40°C. Microbial agents can be used to prepare pesticides or fertilizers.

[0104] figure 1 It shows the Gram staining results and phage morphology of Bacillus amyloliquefaciens, CGMCC No.13047 strain; it shows that the strain is Gram-positive and rod-sha...

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Abstract

The invention discloses a bacillus amyloliquefaciens and a microbial inoculum, and a production method and application of the microbial inoculum, and belongs to the field of microorganisms. The bacillus amyloliquefaciens (CGMCC (China General Microbiological Culture Collection Center) No.13047) has the disease prevention and growth promoting capability. The bacillus amyloliquefaciens with biological activity is inoculated in a solid substrate to be fermented; an obtained fermentation product can be directly used as a bacillus amyloliquefaciens microorganism inoculants; the microbial inoculum has the bacteriostasis effect and can be used as a microbial pesticide; phoma foveata, colletotrichum coccodes, fusarium avenaceum and fusarium chlamydosporum can be inhibited; the bacteriostasis rate is 67 percent or higher, so that the defects of poor environmental protection property, easy generation of drug resistance and poor safety of the chemical pesticide in the prior art can be overcome; the advantages of good environmental protection property, no drug resistance and high safety are realized.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an endophytic bacterium bacillus amyloliquefaciens and its application. Background technique [0002] After entering the 21st century, based on the pollution of chemical pesticides to the environment and pesticide residues, with the proposal and practice of the concept of "public plant protection, green plant protection", the research and development of biological pesticides has become a hot spot in my country's biological industry, agricultural research and application, and has been listed As a major research field and direction of the national medium and long-term scientific and technological development plan, green plant protection has become an important demand and inevitable trend of national development. [0003] Existing biopesticides are mostly produced by fermentation of agricultural by-products or agricultural waste. On the one hand, the biopesticides produced a...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C05F11/08C12R1/07
CPCA01N63/00C05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 杨成德陈秀蓉薛莉柏玉晶
Owner GANSU AGRI UNIV
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