Application of trichoderma strains in preparation of biopesticide for preventing and controlling cucumber fusarium wilt

A cucumber fusarium wilt and bio-pesticide technology is applied in the field of microorganisms to achieve the effects of fast growth, large spore production, and enhanced specialization

Inactive Publication Date: 2013-09-11
侨昌生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no specific and efficient product for the control of cucumber wilt in Trichoderma preparation products

Method used

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  • Application of trichoderma strains in preparation of biopesticide for preventing and controlling cucumber fusarium wilt
  • Application of trichoderma strains in preparation of biopesticide for preventing and controlling cucumber fusarium wilt
  • Application of trichoderma strains in preparation of biopesticide for preventing and controlling cucumber fusarium wilt

Examples

Experimental program
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Effect test

Embodiment

[0022] 1. Acquisition of Trichoderma harzianum SH2303CGMCC NO.4963

[0023] For 1032 wild strains of Trichoderma isolated from the soil of vegetable protection land in East China, the applicant selected the growth rate of Trichoderma, the amount of sporulation and the inhibition rate of pathogenic bacteria in vitro for preliminary screening, and then passed the non-volatile substances and volatiles. Trichoderma harzianum (Trichoderma harzianum) SH2303CGMCC NO.4963 .

[0024] The specific process is as follows: Collect soil from vegetable protection land in East China→soil preservation at 4°C→isolate and purify strains (1032 strains in total)→primary screening indicators (growth speed, spore production and pathogenic bacteria inhibition rate against in vitro confrontation)→re-screening (Detection of non-volatile substances and volatile substances, detection of chitinase activity)→In vivo inoculation control effect test. After repeated screening through the above steps, a Tric...

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Abstract

The invention relates to the application of trichoderma strains in preparation of biopesticide for preventing and controlling cucumber fusarium wilt. The trichoderma strains are Trichoderma harzianum SH2303CGMCC NO.4963. The main components for preventing and controlling the cucumber fusarium wilt are conidia of the Trichoderma harzianum SH2303; and the biopesticide can be seed coating agents or granules. The trichoderma strains in the application has high growth speed and high sporulation quantity; the confrontation pathogens inhibition rate in vitro reaches 43.01 percent; the inhibition rate of a non-volatile inhibitor to the cucumber fusarium wilt is 41.33 percent; the inhibition rate of a volatile inhibitor to the cucumber fusarium wilt is 11.49 percent; the chitinase activity is 3.9573U; the beta-1,3-glucanase enzyme activity is 0.5092U; the extracellular protease enzyme activity is 3.0678 U; the prevention effect aiming at the living bodies of the cucumber fusarium wilt is 71.43 percent; the overall proportion of the antibiosis secondary metabolites, having antagonism, in the strains is 30.28 percent; and the trichoderma strains can effectively prevent and treat the disease of the cucumber fusarium wilt.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to the use of a Trichoderma strain in the preparation of biological pesticides for the prevention and treatment of cucumber wilt. Background technique [0002] Cucumber (Cucumis sativus L.) is one of the main melon vegetables grown in protected cultivation in my country. Due to the difficulty of falling stubble in protected cultivation and the increasingly serious obstacles to continuous cropping, cucumber fusarium wilt, a common and difficult to control soil-borne disease, seriously affects cucumber production. Cucumber Fusarium wilt, also known as vine wilt and wilting disease, is a soil-borne and root-invasive vascular disease. The general incidence rate is 20%-30%, and it is as high as 80%-90% in severe plots, or even completely destroys the species. In recent days, due to the expansion of cucumber cultivation area in my country, cucumber wilt has occurred alm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04C12N1/14A01P3/00C12R1/885
CPCY02A50/30
Inventor 陈捷孙瑞艳刘志诚李雅乾姚力美林振亚
Owner 侨昌生物科技(上海)有限公司
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