34results about How to "Result Reliability Guarantee" patented technology

The present invention is one kind of primer for fast molecular detection of root nematode, especially for fast molecular detection and identification of Southern, Java and peanut root nematode, and belongs to the field of crop disease and pest prevention and treatment and plant quarantine technology. Three pairs of primer, including MI-F/R, MJ-F/R and MI-F/MT-R, are designed for specifically proliferation of products with 955 bp, 517 bp and 799 bp in colony of Southern root nematode, Java root nematode and Southern, Java and peanut root nematode separately. The three pairs of primer has proliferation sensitivity of 1/3 two-instar larva, male adult and female adult. These primers may be used in fast detection and identification of root nematode in soil sample and root sample.

The invention discloses a method for identifying helix pomatia by PCR (polymerase chain reaction) detection, which uses specific primers to identify molecular characteristics of helix pomatia. The forward primer for the PCR reaction is HP-(P1): 5'-TTCGTTGAGAGTTAGG-3', and the reverse primer is HP-(P2): 5'-ATAGTAATAGCACCAGC-3'. The total volume of the PCR reaction system is 25 mu L, wherein the 2*TaqPCRMasterMix is 12.5 mu L, the forward and reverse primers are respectively 0.5 mu L, the DNA (deoxyribonucleic acid) template is 2 mu L, and the rest is sterilized ddH2O. The PCR reaction process comprises the following steps: pre-denaturating at 5 DEG C for 5 minutes; treating at 95 DEG C for 30 seconds, 45 DEG C for 30 seconds and 72 DEG C for 1 minute, and repeating 30 times; and extending at 72 DEG C for 10 minutes, and finishing the reaction. The method disclosed by the invention can quickly and accurately detect and identify helix pomatia. The invention provides a new technical means for detection and identification of helix pomatia in the Catalogue of Quarantine Pest for Import Plants to the People's Republic of China, and has important meanings for preventing helix pomatia from spreading and diffusion.

The invention discloses a phytophthora capsici LAMP (Loop-mediated isothermal Amplification) primer and a rapid detection method thereof, which are specially used for phytophthora capsici specific detection. The invention mainly designs a phytophthora capsici LAMP primer (F3, B3, FIP and BIP); the primer is subjected to isothermal amplification and a 50micromole/L Calcein-500micromole/L MnCl2 developing agent is added to develop or agarose gel electrophoresis detection is carried out, so as to observe green fluorescent light or a ladder belt with an LAMP characteristic. The phytophthora capsici LAMP primer and the rapid detection method thereof can be used for rapid, sensitive and accurate detection of plants infected by phytophthora capsici and the phytophthora capsici in the soil in production practice and can also be used for early diagnosis of field diseases and the monitoring and identifying of bacteria, so as to provide reliable technological and theoretical foundations for preventing and treating the diseases caused by the phytophthora capsici.

The invention discloses a peronophythora litchii LAMP (loop-mediated isothermal amplification) primer and a rapid detection method thereof which are specially applied to specific detection of peronophythora litchii. The peronophythora litchii LAMP primer is mainly adopted and designed, and through isothermal amplification visual inspection or agarose gel electrophoresis detection, green fluorescence can be observed or an LAMP characteristic ladder can appear. The LAMP primer and a usage method thereof can be applied to rapid, sensitive and accurate detection of the peronophythora litchii in plants infected with the peronophythora litchii in production practice, can also be applied to early diagnosis of diseases and pests in fields as well as monitoring and identification of germs, and provide reliable technical and theoretical bases for control of diseases and pests caused by the peronophythora litchii.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) primer of phytophthora nicotianae and a fast detection method thereof, which are especially used for specific detection of the phytophthora nicotianae. The LAMP primer adopted and designed is F3, B3, FIP and BIP, and the sequence of the LAMP primer is shown in SEQIDNO.1-4. Green fluorescence can be observed or the ladder pattern of LAMP characteristic occurs by isothermal amplification and addition of SYBRgreenI color-developing agent for color development or sepharose for gel-electrophoresis detection. The LAMP primer and the use method can be used for fast, sensitive and accurate detection of the phytophthora nicotianae in plants and soil infected by the phytophthora nicotianae in production practice, simultaneously can be used for early diagnosis of diseases in the field and monitoring and identification of germs, and provide reliable technical and theoretical basis for controlling the diseases caused by the phytophthora nicotianae.

The invention discloses a method for detecting and identifying Helix aspersa MUller through PCR (polymerase chain reaction). The method comprises the step of identifying the molecular feature of the Helix aspersa MUller by using specific primers, wherein the upstream primer of PCR is HP-(P1): 5'-ACTCTTTGGTATTTGATGCG-3', and the downstream primer is HP-(P2): 5'-ACCTGCTAAAACGGGTAATG-3'. The method can be used for quickly and accurately detecting and identifying the Helix aspersa MUller. The method provides a new technical means for detection and identification of the Helix aspersa MUller in Imported Plant Quarantine Pest Directory of the People's Republic of China, and has significance for preventing the propagation and diffusion of the Helix aspersa MUller.

The invention provides a LAMP detection primer for sweet potato blast fungus and a visual detection method of the blast fungus, wherein a group of LAMP primers F3, B3, FIP and BIP of sweet potato blast fungus are mainly designed; LAMP is used for constant temperature amplification; after the amplification reaction, chromogenic reaction or agarose gel electrophoresis detection can be performed to directly and visually observe characteristics of ladder patterns in fluorescent green or electrophoresis. Loop-mediated isothermal amplification technology established by the invention for the sweet potato blast fungus can realize rapid detection of the sweet potato blast fungus in diseased tissues (plants and potato pieces), has advantages of rapid amplification, high efficiency, good specificityand convenient operation, no need of special instruments, and provides a reliable technical basis for prevention and control of the sweet potato blast fungus.

The invention discloses phytophthora vignae LAMP (loop-mediated isothermal amplification) detection primers and a phytophthora vignae LAMP detection method which are specially applied to specific detection of phytophthora vignae. The phytophthora vignae LAMP primers are mainly adopted and designed, and green fluorescence can be observed or an LAMP characteristic ladder can appear through the chromogenic reaction or agarose gel electrophoresis detection after LAMP. The LAMP primers and a usage method thereof can be applied to rapid, sensitive and accurate detection of the cowpea diseases in production practice, can also be applied to early diagnosis of diseases in fields as well as monitoring and identification of germs, and provide reliable technical and theoretical bases for control of the cowpea diseases.

The invention discloses a primer group utilizing an LAMP technology to detect witloof pseudomonas and a kit and a method. The primer group includes a pair of outer-side primers F3 and B3 and a pair of inner-side primers FIP and BJP, and sequences are respectively shown as SEQ IDNO.1-4. The kit includes the primer group and can also include other detection reagents. The method utilizing the LAMP technology to detect the witloof pseudomonas comprises the steps that a genome DNA of a sample to be tested is used as a template, and LAMP amplification reaction is performed by utilizing the primer group or the kit; after the reaction, the color change of an LAMP amplification reaction solution is observed, if the reaction solution is a green troubled solution, the reaction is regarded as positive reaction; if the reaction solution is an orange transparent solution, the reaction is regarded as negative reaction. The primer group has the following advantages that a result is reliable, the specificity is strong, the sensitivity is high, and the primer group is fast and efficient and simple, convenient and easy to operate.