Banana fusarium wilt antagonistic strain, bio-organic fertilizer, preparation method and application thereof

A technology for banana fusarium wilt and bio-organic fertilizer, which is applied in the preparation of organic fertilizer, biochemical equipment and methods, and botanical equipment and methods, etc. The effect of increasing resistance, increasing the number, significant slab suppression effect

Active Publication Date: 2017-11-03
INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the late start of research on the prevention and control of banana wilt in my country, the existing resource bank of bio-control bacteria is limited, an

Method used

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  • Banana fusarium wilt antagonistic strain, bio-organic fertilizer, preparation method and application thereof
  • Banana fusarium wilt antagonistic strain, bio-organic fertilizer, preparation method and application thereof
  • Banana fusarium wilt antagonistic strain, bio-organic fertilizer, preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The source of the screening bacteria for the prevention and control of antagonistic bacteria against banana wilt was the rhizosphere soil of healthy banana plants in the diseased banana garden, and the physiological race 4 of the pathogen of banana wilt was used as the indicator bacteria. The antagonistic bacteria with obvious plate inhibitory effect is numbered H-6; a single colony of the antagonistic bacteria was obtained by streaking purification.

[0056] 1. Morphological and physiological and biochemical identification of H-6

[0057] Pick the antagonistic bacteria H-6 on the LB slant, inoculate it on the LB plate and streak, culture at 30°C for 36 hours, and observe the single colony morphology. Physiological and biochemical identification of antagonistic bacteria was carried out using HBI microbial biochemical identification strips (Haibo Biological Company, Qingdao). Strain Gram staining was carried out according to the "Common Bacteria Identification Manual". ...

Embodiment 2

[0067] Bacterial agent preparation: Pick a single colony of H-6 antagonistic bacteria, inoculate it in a fresh LB test tube culture medium, and culture it on a shaking table overnight to obtain antagonistic bacteria seed liquid, the culture condition is 30°C, 170r / min; the above seed liquid According to the ratio of 2%, it was transferred to the Erlenmeyer flask equipped with fresh LB medium, and the shaking table was cultivated for 3 days, and the number of detected antagonistic bacteria reached 1.0×10 9 After cfu / mL, the bacterial solution was centrifuged under the following conditions: 10000r / min, 5min, the supernatant was discarded, the bacterial cells were collected, resuspended with an equal volume of deionized water, and the initial number of antagonistic bacteria was 1.0×10 9 cfu / mL.

[0068] Biological organic fertilizer: Mix 50Kg of cow dung composting products with 5Kg of peanut bran and 5Kg of rapeseed meal evenly, add 12L of antagonistic bacteria to adjust the wat...

Embodiment 3

[0076] Prepare antagonistic bacteria: pick a single colony of H-6 antagonistic bacteria, inoculate it in a fresh LB test tube culture medium, and culture it overnight on a shaking table to obtain antagonistic bacteria seed liquid. The culture conditions are 30°C, 170r / min; The seed solution was transferred to the Erlenmeyer flask with fresh LB medium according to the ratio of 2%, and cultured on a shaker for 3 days, the culture condition was 30°C, 170r / min; the amount of antagonistic bacteria in the Erlenmeyer flask was detected to reach 1.0× 10 9 After cfu / mL, the bacterial liquid was centrifuged under the following conditions: 10000r / min, 5min, the supernatant was discarded, the bacterial cells were collected, and the bacterial cells were resuspended with deionized water equal to the volume of the original bacterial liquid, and mixed thoroughly to obtain the Antagonistic bacterial agent, the initial quantity of antagonistic bacteria in the bacterial agent is 1.0×10 9 cfu / mL...

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Abstract

The invention relates to the field of biological prevention and control of banana diseases, in particular to a banana fusarium wilt antagonistic strain, a bio-organic fertilizer, a preparation method and application thereof. The banana fusarium wilt antagonistic strain is preserved in China General Microbiological Culture Collection Center on January 9th, 2017, the preservation number is CGMCC NO.13549, and the preservation name is Bacillus velezensis H-6. According to the invention, a banana fusarium wilt pathogenic bacteria 4# physiological race is adopted as an indicating bacterium from healthy banana rhizosphere soil of an onset banana garden, flat preliminary screening and secondary screening are carried out to obtain the Bacillus velezensis H-6 with strong flat inhibition effect on banana fusarium wilt pathogenic bacteria, the Bacillus velezensis H-6 can be prepared into a bio-organic fertilizer for prevention and control of banana fusarium wilt, and further study on the strain in improving the prevention and control effect of biocontrol bacteria can enrich the existing fusarium wilt biocontrol bacteria resource pool.

Description

technical field [0001] The invention relates to the field of biological prevention and control of banana diseases, in particular to a banana fusarium wilt antagonist, bio-organic fertilizer and a preparation method and application thereof. Background technique [0002] Banana Fusarium wilt is a devastating soil-borne fungal disease caused by the infection of Fusarium oxysporum f.sp.cubense and causes the necrosis of banana vascular bundles. The disease will cause the stems and leaves of banana plants to wilt or even die completely. Difficult to contain, seriously affected the development of the banana industry. [0003] The reported methods for the prevention and control of Fusarium wilt of bananas mainly include the cultivation of disease-resistant varieties, agronomic control, chemical control, and biological control. The first three measures all have problems such as polluting the secondary environment, high cost, and unstable control effects. , and biological control is...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C05F15/00C05F17/00C12R1/07
CPCA01N63/00C05F3/00C05F17/00C12N1/20C12N1/205C12R2001/07C05F5/002C05F11/08Y02W30/40
Inventor 黄建凤张发宝逄玉万黄巧义唐拴虎杨苞梅
Owner INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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