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Paenibacillus polymyxa NSY50 with capabilities of promoting growth and preventing diseases

A polymyx spore-like and biocontrol strain technology is applied in the prevention and control of cucumber fusarium wilt, promotes the growth of cucumber seedlings, can solve the problems of lack of disease-resistant varieties, heavy workload and high cost, and achieves reduction of cucumber fusarium wilt. The effect of generating, reducing utilization and dependence, and saving production costs

Inactive Publication Date: 2016-07-06
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the pathogen can survive in the soil for several years, which greatly affects the economic benefits of farmers farming
Due to the lack of disease-resistant varieties, the use of chemical pesticides such as methyl bromide soil fumigation and carbendazim in traditional agricultural production is likely to cause problems such as environmental pollution, product safety, and crop disease resistance.
However, measures such as grafting and root replacement, crop rotation and even soil replacement are cumbersome, heavy workload, and high cost. Therefore, it is of great significance to use safe and efficient biocontrol agents to prevent and control cucumber wilt.

Method used

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  • Paenibacillus polymyxa NSY50 with capabilities of promoting growth and preventing diseases
  • Paenibacillus polymyxa NSY50 with capabilities of promoting growth and preventing diseases
  • Paenibacillus polymyxa NSY50 with capabilities of promoting growth and preventing diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The isolation of embodiment 1 Paenibacillus polymyxa NSY50

[0029] Screening method: It is separated from the matrix of vinegar grains which has a good control effect on cucumber wilt.

[0030] Among them, the vinegar grain base is provided by Zhenjiang Peilei Base Technology Development Co., Ltd. The raw material comes from the waste of the local vinegar brewing industry, and the vinegar grain base is made through fermentation. The separation method is carried out according to the following steps:

[0031] (1) Preliminary selection of antagonistic bacteria: the mass volume ratio (g / mL) of vinegar grains matrix and sterile water was 1:10, cultured with shaking at 28°C and 180 rpm for 6-8 hours, and filtered through filter paper to obtain the extract. In the ultra-clean workbench, spread 100 μL of the extract solution on a 90mm petri dish containing solid PDA medium, air-dry it for 5-10 minutes, and transfer the pre-cultivated Cucumber wilt bacteria block with a diamet...

Embodiment 2

[0035] The identification of embodiment 2 Paenibacillus polymyxa NSY50

[0036] Identification method: The strain was identified through morphological characteristics, physiological and biochemical experiments and 16SrDNA sequence analysis.

[0037] Scanning electron microscopy was used to observe the shape and size of the bacteria (see figure 1 ).

[0038] The strain NSY50 obtained by screening was activated on LB medium, then transferred to 50ml LB culture medium, and cultured with shaking at 28°C and 180rpm for 24-48h to obtain activated NSY50 bacterial liquid, which is ready for use.

[0039] Detection of IAA-producing ability: Add 100 g / L tryptophan to sterilized LB liquid medium, inoculate 0.2 ml of activated NSY50 bacterial solution, use Salkowski method to measure IAA-producing ability, and measure absorbance at 540 nm (Table 1).

[0040] Inoculate 5 μL of NSY50 bacterial solution in the center of protease detection medium plate and cellulase detection medium plate r...

Embodiment 3

[0054] Example 3 Detection of broad-spectrum antagonistic ability of Paenibacillus polymyxa NSY50

[0055] The antibacterial spectrum of NSY50 is measured by the four-point method of plate confrontation: the activated NSY50 bacterium liquid (prepared in Example 2) is inoculated into the bacteria containing cucumber wilt (Fusariumoxysporum f.sp.Cucumberinum), melon blight (Didymelabryoniae ), wheat scab (Fusarium graminearum), tomato Ralstonia solanacearum (Ralstonia solanacearum) on the PDA plate, sealed with a parafilm, cultivated in a biochemical incubator at 28°C for 5-7 days, and observed whether there was a bacteriostatic zone formed on the plate, If there is a transparent inhibition zone, it means that Paenibacillus polymyxa NSY50 has an inhibitory effect on the pathogen. see results image 3 , the transparent circles appeared on the above four PDA plates, indicating that Paenibacillus polymyxa NSY50 has inhibitory effects on cucumber wilt, melon wilt, wheat scab and to...

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Abstract

The invention belongs to the field of biotechnology, and discloses a paenibacillus polymyxa NSY50 which is preserved in the China General Microbiological Culture Collection Center on March 8, 2016 and has a preservation number of CGMCC NO. 12189. The invention also discloses a microbial inoculum containing the paenibacillus polymyxa NSY50. The strain disclosed by the invention can be used for synthesizing IAA, can generate protease and cellulase, has activity of ACC desaminase, and has a relatively good inhibitory effect on various plant pathogens of fusarium oxysporum f.sp.cucumberinu, didymella bryoniae, fusarium graminearum, ralstonia solanacearum and the like. A greenhouse experiment shows that the microbial inoculum prepared by using the strain can significantly promote the growth of cucumber seedlings and reduce the incidence of fusarium wilt of cucumber, and by adopting a bio-control microbial inoculum prepared by adopting the strain to perform root irrigation during transplantation, the bio-control effect on the fusarium wilt of cucumber under a room temperature condition reaches 75.90-82.83%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a strain of Paenibacillus polymyxa NSY50, a bacterial agent containing Paenibacillus polymyxa NSY50, and the application of the bacterial agent in promoting the growth of cucumber seedlings and preventing and treating cucumber wilt. Background technique [0002] Cucumber is a globally important vegetable crop with a protected area of ​​620,000 hectares. It is the largest cultivated crop in protected areas. my country is the country with the largest cucumber production area and the highest total output in the world. In recent years, soil-borne fusarium wilt has become more and more serious in facility cucumber cultivation, and its pathogen is Fusarium oxysporum f. sp. Cucumerinum. In recent years, it has generally occurred in the main cucumber planting areas in China, which can generally lead to a 10% to 30% reduction in cucumber production, and can reach 80% to 90% in severe cases. At...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 郭世荣杜南山施露孙锦束胜
Owner NANJING AGRICULTURAL UNIVERSITY
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