Acinetobacter with authigenic nitrogen fixation capacity and application thereof

A bacillus and nitrogen fixation technology, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of inability to meet the requirements of polluted soil remediation, resistance to heavy metal growth and nitrogen fixation, and slow growth.

Inactive Publication Date: 2014-03-26
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most wild nitrogen-fixing bacteria have the disadvantages of slow growth, heavy metal resistance and low nitrogen fixation ability, which cannot meet the needs of contaminated soil remediation

Method used

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  • Acinetobacter with authigenic nitrogen fixation capacity and application thereof
  • Acinetobacter with authigenic nitrogen fixation capacity and application thereof
  • Acinetobacter with authigenic nitrogen fixation capacity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] According to the screening method of the nitrogen-fixing bacterial strain provided by the present invention, a nitrogen-fixing bacterial strain with high nitrogenase activity capable of tolerance to heavy metal cadmium is selected, and the preparation steps are as follows:

[0062] 1) Use the autogenous nitrogen-fixing bacterium Acinetobacter YSGD07 screened from the plant rhizosphere in the laboratory as the starting strain;

[0063] 2) Mutation breeding

[0064] (1) Prepare the single cell suspension of the starting strain YSGD07

[0065] The starting strain YSGD07 was inoculated in liquid medium A, cultured at 28°C, 150rpm for 16hrs, centrifuged, washed with sterile saline, placed in a conical flask with glass beads, and oscillated to disperse into a single-cell bacterial suspension liquid;

[0066] The composition of the medium A is: yeast powder 10g, NaCl 5g, peptone 10g, FeSO 4 ·7H 2 O 0.005g, Na 2 MoO 4 2H 2 O 0.0025g, distilled water 1000ml, pH 7.0~7.2.

...

Embodiment 2

[0078] According to the method for preparing nitrogen-fixing bacterial agent provided by the present invention, the nitrogen-fixing bacterial agent with high activity of nitrogenase that can tolerate heavy metal cadmium is prepared, and the steps of preparation are as follows:

[0079] The preparation of bacterial agent comprises the following steps:

[0080] 1. Strain fermentation

[0081] ①First-level Erlenmeyer flask liquid culture

[0082] The frozen Ymu7-3 was quickly thawed at 37°C, and transferred into the Erlenmeyer flask containing liquid medium A according to the inoculum amount of 0.5%, and cultured at 28°C with shaking at 150rpm for 18hrs;

[0083] The composition of the liquid medium A is: the composition of the medium A is: yeast powder 10g, NaCl 5g, peptone 10g, FeSO 4 ·7H 2 O 0.005g, Na 2 MoO 4 2H 2 O 0.0025g, distilled water 1000ml, pH 7.0~7.2

[0084] ②Secondary fermentation tank fermentation

[0085] 1) Put the primary culture medium of Ymu7-3 into ...

Embodiment 3

[0090] Use embodiment 2 bacterial agents to repair cadmium-contaminated soil in strengthened Indian mustard, and its specific steps are:

[0091] The potting soil was collected from the farmland soil in Chang’an County, Shaanxi Province. The total Cd content of the tested soil was 0.25 mg / kg, and 4 soils were added with Cd 2+ levels, respectively 0, 20, 40, 80mg / kg, the corresponding amount of CdCl 2 Dubbed into a solution, so that the test soil and heavy metals are repeatedly mixed evenly, and left to balance for 30 days. In the experiment, a blank control group, a urea-added group and a bacterial agent-added group were respectively set up. After emergence, keep 3 plants in each pot. Dilute the liquid bacterial agent prepared above according to the ratio of 1:100, and use the method of root irrigation to pour 50ml of the diluted bacterial liquid in each pot, once every 3 weeks, without adding bacteria The group poured the same dose of dead bacteria, and the concentration o...

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Abstract

The invention discloses an acinetobacter with authigenic nitrogen fixation capacity, which is classified and named as Ymu7-3 and is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC No. 5006. The free living nitrogen fixing bacteria has high nitrogen fixing capacity, is capable of resisting the growth of Cd with higher concentration, and can secrete heteroauxin so as to generate iron atom; and the prepared microbial bacterium agent can remarkably promote the growth of hyper-accumulating plants, increase the biomass liveweight of the hyper-accumulating plants, greatly improve the heavy metal enrichment factor and transfer coefficient of the hyper-accumulating plants, and improve the ecological environment of the soil, thus being environment-friendly and low in cost.

Description

technical field [0001] The invention relates to a strain of Acinetobacter with self-generated nitrogen-fixing ability. The Acinetobacter is obtained by ultraviolet-plasma compound mutagenesis, has the ability to resist the growth of heavy metals, can be used for environmental treatment of soil heavy metal pollution, and belongs to the field of microbial application technology. Background technique [0002] Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds, mainly caused by human factors such as mining, waste gas discharge, and sewage irrigation. In recent years, with the growth of population, the rapid development of industry, and the extensive use of chemical fertilizers and pesticides, soil heavy metal pollution has become increasingly serious. [0003] Cd is a highly toxic heavy metal. The Japanese "Itai-Itai Disease" that shocked the world was caused by cadmium pollution. Cadmium will replace calcium in bone, softening and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C05F11/08C12R1/01
Inventor 朱晓丽马沛范代娣申烨华梁丽华
Owner NORTHWEST UNIV
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