A sulfate-reducing bacterium-phosphorus solubilizing bacteria and its application in combined remediation of cadmium-contaminated soil

A technology of liquid inoculum and microbial inoculum, applied in the field of environmental microorganisms, can solve the problems of wild-type SRB inhibition, increase of Cd mobility, etc., and achieve the effect of promoting plant growth and increasing yield

Active Publication Date: 2017-11-17
西安金博瑞生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the growth of wild-type SRB was inhibited in cadmium-contaminated soil, and the formed CdS was easily oxidized to CdSO under aerobic conditions. 4 , which increases the mobility of Cd

Method used

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  • A sulfate-reducing bacterium-phosphorus solubilizing bacteria and its application in combined remediation of cadmium-contaminated soil
  • A sulfate-reducing bacterium-phosphorus solubilizing bacteria and its application in combined remediation of cadmium-contaminated soil
  • A sulfate-reducing bacterium-phosphorus solubilizing bacteria and its application in combined remediation of cadmium-contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Mutation Breeding of Efficient Cadmium Sulfate Reducing Strains

[0057] (1) The method for mutagenesis and breeding of the SRB bacterial strain provided by the invention comprises the following steps:

[0058] 1) A facultative anaerobic bacterium SRB-2 screened from Cd-contaminated soil in our laboratory was used as the starting strain;

[0059] 2) Mutation breeding

[0060] (1) Prepare the single cell suspension of the starting strain SRB-2

[0061] Inoculate the starting strain SRB-2 in liquid medium A, cover it with sterile liquid paraffin at 28-32°C, let it stand for 36 hours, centrifuge, wash with sterile saline, and place it in a Erlenmeyer flask equipped with glass beads , oscillating to disperse into a single-cell bacterial suspension;

[0062] Described liquid medium A consists of: K 2 HPO 4 0.5-0.8g, (NH 4 ) 2 SO 4 2.5-3.0g, NaHCO 3 0.3-0.5g, CaCl 2 0.2-0.3g, MgSO 4 1.0-1.5g, yeast extract 1.0-1.5g, sodium lactate 1.0-2.0ml, distille...

Embodiment 2

[0090] Example 2 Mutation Breeding of Cadmium-tolerant Phosphorus Solubilizing Strains

[0091] (1) The method for mutagenesis and breeding of the LB4-4-1c strain provided by the present invention comprises the following steps:

[0092] 1) LB-4, which was screened from the rhizosphere of plants in Cd-contaminated soil in the laboratory, was used as the starting strain;

[0093] 2) Mutation breeding

[0094] (1) Prepare a single-cell suspension of the starting strain LB-4

[0095] Inoculate the starting strain LB-4 in liquid medium C, culture at 28-32°C, 150-180rpm for 8-12 hours, centrifuge, wash with sterile saline, place in a Erlenmeyer flask with glass beads, shake, Make it dispersed into a single-cell bacterial suspension;

[0096] The composition of the liquid medium C is: tryptone 5-10 g, yeast powder 3-5 g, NaCl 5-10 g, distilled water 1000 ml, pH value 6.8-7.5, sterilized at 121°C for 20 minutes.

[0097] (2) Ultraviolet mutagenesis

[0098] Adjust the concentrati...

Embodiment 3

[0125] Example 3 Determination of SRB-2-5u-2b reducing sulfate activity

[0126] Inoculate SRB-2-5u-2b into a test tube containing 10ml of liquid medium A, cover it with liquid paraffin, and culture it statically at 35°C for 20 hours, take 100 μl (dilute the bacterial solution with liquid medium A to make its OD600 0.3) Bacterial solution was inoculated in liquid medium A at 35°C, under anaerobic or aerobic conditions, and cultured statically for 7 days, and the sulfate reduction activity was determined by barium sulfate precipitation method. The anaerobic conditions are as follows: add 200ml of liquid medium A to the anaerobic culture bottle, insert SRB-2-5u-2b into the anaerobic culture bottle respectively, and add 20ml of liquid paraffin sealing solution on the liquid surface On the other hand, culture at 35°C in an anaerobic glove box for 7 days; the aerobic conditions are as follows: add 200ml liquid medium A to the Erlenmeyer flask, insert SRB-2-5u-2b under aerobic condi...

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Abstract

The invention discloses bacterial strain b with anti-heavy metal Cd growth and sulfate reduction activity and a bacterial strain with anti-heavy metal Cd growth and phosphorus-solubilizing ability, which are respectively named as Enterbacter ludwigii SRB-2-5u ‑2 and Pseudochrobactrum saccharolyticum (Pseudochrobactrum saccharolyticum) LB‑4‑4‑1c, which has been preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms. The invention can restore the soil surface layer, deep layer and plant rhizosphere soil at the same time, reduce the pollution of surface water and underground water by Cd, and can also improve the activity and fertility of soil.

Description

technical field [0001] The invention relates to a sulfate-reducing bacterium-phosphorus solubilizing bacterium and its application in joint restoration of cadmium-contaminated soil, which belongs to the technical field of environmental microorganisms. Background technique [0002] Cadmium is one of the most mobile and toxic heavy metal elements in the environment. Currently, the global annual emissions are about 3.0×10 4 t Cadmium, in the past 50 years, about 2.20×10 Cd was released into the global environment 4 t, 94% of which entered the soil, resulting in different degrees of Cd pollution in the soil of countries all over the world. According to statistics, the farmland polluted by Cd in my country has exceeded 20×10 4 hm 2 , involving 25 regions in 11 provinces and cities, producing 14.6×10 agricultural products with excessive Cd content every year 8 kg. The cadmium content in polluted paddy fields in Hunan Province was 2.8-51.3 mg / kg, which was 9.3-171 times of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 朱晓丽梁丽华李贺马俊杰
Owner 西安金博瑞生态科技有限公司
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