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Methylotrophic bacillus strain and application thereof

A methylotrophic, bacillus technology, applied in the direction of application, bacteria, fungicides, etc., can solve the problem of single function of bacillus, achieve good development and application prospects, reduce the requirements for storage and transportation environment, and have a wide range of applications

Active Publication Date: 2015-11-11
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the functions of these bacillus are relatively simple, and the isolation and screening of high-efficiency strains with antagonistic effects on various plant pathogenic bacteria is the hot spot and key issue of current research.

Method used

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  • Methylotrophic bacillus strain and application thereof
  • Methylotrophic bacillus strain and application thereof
  • Methylotrophic bacillus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The screening and identification of methylotrophic bacillus strain CGMCCNo.10779 comprises the following steps:

[0028] (1) Activation of pathogenic bacteria

[0029] Use the inoculation needle to aseptically pick up a small amount of mycelium and culture medium to be activated and inoculate the center of the PDA plate, inoculate 3 pieces respectively, and carry out constant temperature cultivation under the condition of 26°C to prepare the spore suspension;

[0030] (2) Sample collection and preparation of soil suspension

[0031] Bacterial samples were collected from the soil of willow fields in Xuancheng area. When collecting soil samples, first remove the topsoil and take soil samples at a depth of 5-20cm; use the dilution method to separate, weigh 10.0g of soil samples and pour them into 100ml of sterile water Shake well in the Erlenmeyer flask, then put it into an 80°C water bath for heating (shake well every 3 minutes), put it in cold water to cool down after 2...

Embodiment 2

[0053] Effects of methylotrophic Bacillus strain CGMCCNo.10779 on the growth of black line anthracnose, colloid anthracnose, watermelon wilt, cotton wilt, and corn leaf spot

[0054] 2.1 Determination of the antibacterial rate of methylotrophic Bacillus strain CGMCCNo.10779 against black line anthracnose, colloid anthracnose, watermelon wilt, cotton wilt, and corn leaf spot

[0055] The inhibitory effect of methylotrophic Bacillus strain CGMCCNo.10779 on black line anthracnose, colloid anthracnose, watermelon wilt, cotton wilt, and corn leaf spot was detected by plate confrontation method. The test method is as follows: inoculate plant pathogenic bacteria on a PDA plate, culture at 26°C for 3 days, use a puncher with a diameter of 5mm to get the bacteria blocks with consistent growth of colonies, plant them in the center of the PDA plate, and draw a line at 2cm on the side of the bacteria. Inject the methylotrophic bacillus strain CGMCCNo.10779, take only the pathogenic bacter...

Embodiment 3

[0066] Effects of Methylotrophic Bacillus Strain CGMCCNo.10779 on Mycelium of Black Line Anthracnose and Watermelon Fusarium Wilt

[0067] Inoculate black line anthracnose and watermelon fusarium wilt pathogens on PDA plates, culture at 26°C for 3 days, use a puncher with a diameter of 5mm to get the bacteria blocks with uniform colony growth, and plant them in the center of the PDA plate, 2cm away from the side of the bacteria Inoculate the methylotrophic Bacillus strain CGMCCNo.10779 by streaking, and take only pathogenic bacteria as the control, each treatment has 3 replicates, and culture at a constant temperature of 26°C; after the pathogenic bacteria in the control group cover the entire petri dish, observe its antibacterial effect With or without, observe its inhibitory effect. The hyphae blocks were selected, fixed with glutaraldehyde, processed by conventional methods, and observed by scanning electron microscope. Put the selected mycelium block (5mm×5mm, thickness abo...

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Abstract

A methylotrophic bacillus strain has a preservation number of CGMCC No.10779. The invention further provides the application of the methylotrophic bacillus strain with the CGMCC No.10779 in antagonism phytopathogen, particularly the application in pathogenic bacteria of antagonism anthracnose of ophiopogon japonicus. The methylotrophic bacillus strain provided by the invention has the benefits as follows: 1, the methylotrophic bacillus strain with the CGMCC No.10779 has an antagonistic effect on various plant pathogens such as watermelon fusarium wilt, fusarium wilt of cotton, and helminthosporium maydis, particularly has a very high and efficient antagonistic effect on pathogenic bacteria of anthracnose of ophiopogon japonicus, is classified as an antagonistic strain having high plant disease curing effect and being environmentally friendly and high in biological control, and has an excellent development prospect. 2, the methylotrophic bacillus strain with the CGMCC No.10779 is strong in salt resistance. On the basis that the content of sodium chloride can reach 9 percent, the strain can still grow, is strong in stress-resist ability, is applicable to soil in relatively poor environment, and has a high potential application value.

Description

technical field [0001] The invention belongs to the field of development and application of microorganisms, and in particular relates to a methylotrophic bacillus strain and its application. Background technique [0002] With the requirement of intensive agricultural production and sustainable development, crop diseases and insect pests have become the bottleneck restricting the sustainable development of modern agriculture. Especially soil-borne diseases such as fusarium wilt, verticillium wilt, root rot, sclerotinia, bacterial wilt, etc., which spread through the soil, cause the root and whole plant of crops to disease, and cause serious losses to agricultural production. However, the chemical pesticides used in large quantities have caused soil and water pollution, seriously threatening people's health, and the breeding cycle of disease-resistant varieties is very long. Due to the limitations of the above traditional methods, it is difficult to find an environmentally fri...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
Inventor 杨恩东孙乐妮孙冬冬汪维云蔡永萍陈晓琳孙旭常艳
Owner ANHUI AGRICULTURAL UNIVERSITY
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