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Method for extracting pepstatin through fermenting trametes

A technology of pepsin inhibitor and fermentation liquid, which is applied in the field of bioengineering, can solve the problems of low yield of pepsin inhibitor and cumbersome operation, and achieve the effects of good inhibitory effect, good thermal stability, and avoiding damage

Inactive Publication Date: 2014-12-10
中山安荞生物科技有限公司
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

At present, some scholars have reported the extraction of pepsin inhibitor from Yunzhi mushroom and its purification, but there are disadvantages such as cumbersome operation or low yield of pepsin inhibitor.

Method used

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Embodiment 1

[0027] A method for extracting pepsin inhibitor by fermenting Versicolor versicolor, comprising the following steps:

[0028] (1) Sample pretreatment: take 3g of Versicolor versicolor, freeze-dry to constant weight, crush through a 40-mesh sieve, and take the sample powder under the sieve for later use;

[0029] (2) Cultivation and activation of strains: take the sample powder prepared in step (1) and place it in a slant medium for cultivation at a cultivation temperature of 25°C and a cultivation time of 3 days to obtain activated strains;

[0030] (3) Shake flask fermentation culture: Take the activated strain in step (2) and put it into a fermentation bottle for shake flask fermentation culture. The shaker speed is 200r / min, the culture temperature is 25°C, and the culture time is 3 days to obtain the strain fermentation liquid;

[0031] (4) Extraction of pepsin inhibitor: Ultrasonic cell disruption was performed on the fermented broth of the strain prepared in step (3), th...

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Abstract

The invention relates to a method for extracting pepstatin through fermenting trametes. The method comprises the following steps: freezing and drying a proper amount of trametes to a constant weight, grinding and sieving; placing sieved sample powder in an inclined plane culture medium to carry out culturing; placing activated strains into a fermentation flask to carry out flask shaking fermentation culture so as to obtain strain fermentation liquor; and finally, carrying out ultrasonic cell breakage and centrifuging on the strain fermentation liquor, carrying out decoloration through macroporous resin, taking supernate to carry out dialysis and then carrying out purification on the pepstatin so as to obtain the pepstatin. According to the method, the ultrasonic extracting conditions are strictly limited, so that the maximum release of the pepstatin is ensured, the pepstatin is also prevented from being damaged through ultrasonic extraction for excessively long time at an excessively high temperature, and the extracted pepstatin has a good inhibiting effect.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for extracting pepsin inhibitor by fermentation of versicolor versicolor. Background technique [0002] The active center of aspartic acid protease is generally composed of two aspartic acid residues, and currently mainly includes pepsin, chymosin, and lysosomal enzymes. Aspartic protease inhibitors can enhance immunity, fight cancer and lower blood pressure, and also have significant curative effects on patients with senile dementia and gastric ulcer, and are currently widely used in the food health care and pharmaceutical industries. However, the safety of chemically synthesized aspartic protease inhibitors cannot be guaranteed at present. For example, saquinavir can cause lipid metabolism disorders. Therefore, its practical application is obviously limited. [0003] Coriolus versicolor, also known as Coriolus versicolor, Melaleuca versicolor, etc.,...

Claims

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Application Information

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IPC IPC(8): C12P21/00C07K14/81C07K1/36C07K1/34C07K1/18
Inventor 刘巴宁郑安乔
Owner 中山安荞生物科技有限公司
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