Trametes versicolor glucan water agent and preparation method thereof

A technology of versicolor glucan and water preparation, applied in the field of fungicides, can solve the problems of high technical requirements for virus-free plants and resistant varieties, inability to prevent and control potato Y virus in essence, and damage to the ecological environment of farmland, etc., to achieve Not easy to drug resistance, overcome the problem of dust pollution, the effect of broad application prospects

Active Publication Date: 2013-05-01
西安恒田生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cultivation of virus-free plants and resistant varieties requires high technical requirements and low efficiency. Since potato virus Y is prone to resistance, it can only play a role in a short period of time.
Potato virus disease is prevented and controlled by eliminating aphids, which destroys the ecological environment of the farmland, and is prone to produce chemical pesticide residues, polluting the soil
It can be seen that the current field management methods are very passive to reduce the harm of potato virus Y, and cannot essentially prevent and control potato virus Y

Method used

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  • Trametes versicolor glucan water agent and preparation method thereof
  • Trametes versicolor glucan water agent and preparation method thereof
  • Trametes versicolor glucan water agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Preparation of versicolor glucan water:

[0025] Step 1: Fermentation of Yunzhi:

[0026] (1) Shake flask seed activation: Evenly pick the mycelium block of Yunzhi strain with the preservation registration number CGMCC No.6760 and transfer it quantitatively to a 250ml Erlenmeyer flask containing 80ml seed medium, at 28°C, 120r / min Constant temperature shaking culture for 4 days; the formula of the seed medium is: glucose 20g / L, corn flour 10g / L, bran 5g / L, peptone 3g / L, KH 2 PO 4 1.5g / L, MgSO 4 0.2g / L, the rest is water;

[0027] (2) Shake flask seed culture: connect the shake flask seed liquid into a 500ml Erlenmeyer flask equipped with 200ml seed culture medium according to the inoculum size of 50ml / L, at 28°C, 150r / min constant temperature shaking culture for 4d; the seeds The medium formula is: glucose 20g / L, corn flour 10g / L, bran 5g / L, peptone 3g / L, KH 2 PO 4 1.5g / L, MgSO 4 0.2g / L, the rest is water;

[0028] (3) 600L large fermenter cultiva...

Embodiment 2

[0033] Example 2: 1.1% Coriolus versicolor dextran aqueous solution on the control effect experiment of potato virus Y:

[0034] In order to verify the preventive effect of the 1.1% versicolor versicolor dextran water preparation prepared in Example 1 on potato virus Y, we carried out a field extension test from September 2009 to July 2010, and the experimental results are reported as follows:

[0035] 1 Test conditions

[0036] 1.1 Experimental objects, crops and varieties

[0037] The experimental object is Potato virus Y, which comes from the Plant Virus Laboratory of the School of Plant Protection, Northwest Agriculture and Forestry University. The experimental crop is potato, the variety is Zihuabai, and it is purchased from Jindao Seed Agricultural Materials Station, Yangling District, Xianyang City, Shaanxi Province.

[0038] 1.2 Environmental conditions

[0039] The test site is selected in Yangling District, Xianyang City, Shaanxi Province, with an area of ​​800m ...

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Abstract

The invention relates to a trametes versicolor glucan water agent and a preparation method thereof, in particular to a trametes versicolor glucan water agent for potato Y virus. The water agent comprises the following components in percentage by weight: 1.0 to 2.0 percent of trametes versicolor glucan crude product, 5.0 to 8.0 percent of surfactant alkyl glucoside, 3.0 to 5.0 percent of decomposition-preventing agent 1,2-butanediol, 4.0 to 10.0 percent of antifreezing agent glycerol and the balance of double distilled water. The trametes versicolor glucan crude product is prepared by fermenting the trametes versicolor strain with the collection number of CGMCC No.6760 and extracting the glucan. The average control effect of 500-fold solution of the trametes versicolor glucan water agent on the potato Y virus is more than 78 percent, in particular, the average control effect of 1.1 percent trametes versicolor glucan water agent on the potato Y virus is up to 80.4 percent and is more excellent than 30-fold solution of 20 percent gram Y Tring wettable powder.

Description

technical field [0001] The present invention relates to fungicides, in particular to a versicolor glucan aqueous solution and a preparation method thereof, in particular to a versicolor glucan aqueous solution for preventing and treating potato virus Y. technical background [0002] Potato virus Y (Potato virus Y) is one of the viruses that infect potatoes most widely and causes serious economic losses. After potato plants are infected by Y virus, they show symptoms of severe mosaic, leaf vein necrosis and vertical leaf streak necrosis. . Streak necrosis, in particular, can appear on the leaf veins, petioles and stems of the plants. The compound leaves are easy to fall off after being sick, leaving only some leaves near the top of the plants in the later stage, which seriously affects the yield and quality of potatoes. At the same time, potato virus Y can also infect Solanaceae, Amaranthaceae, Leguminosae and Asteraceae crops, and is an important pathogen that harms food cr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01N25/04A01P1/00C08B37/02A01G1/04
Inventor 唐满仓沈小英马玉明安德荣
Owner 西安恒田生物科技有限公司
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