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Trametes hirsute strain and application thereof in production of laccase by using municipal dewatered sludge

A technology of dewatered sludge and Trametes trichotillobacter, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., can solve the problems of difficult microbial cell wall cracking, low microbial utilization efficiency, complex components, etc., and reduce the level of harmful pollutants , improve bioavailability efficiency, the effect of a wide range of sources

Active Publication Date: 2014-07-09
ENERGY RES INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex composition of sludge and the difficulty of breaking the microbial cell wall in sludge, many microorganisms have low utilization efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of application of Trametes trichotis strain in the production of laccase by using municipal dewatered sludge, which includes the following steps of dehydration: (1) Liquid seed medium (g / L): glucose 20, soybean meal 5, yeast powder 1, phosphoric acid di Potassium hydrogen 1, magnesium sulfate heptahydrate 0.5, initial pH 5, shaker speed 200 rpm, inoculated with Trametes trichotillotype SEM-6 (CCTCC No. M 2014002), and cultured for 48 hours to obtain liquid fermented seeds. (2) Liquid fermentation medium (g / L): dried sludge fermentation medium 20 (obtained from Jinan Everbright Water Plant No. The method is as follows: add 0.2 parts by weight of sodium hydroxide to 100 parts by weight of municipal dewatered sludge with a water content of 60%, stir evenly, and treat at 20°C for 2 days to obtain the sludge fermentation substrate. ), potassium dihydrogen phosphate 5, magnesium sulfate heptahydrate 2, bran 5, adjust the initial pH value to 3, and sterilize at 121°C fo...

Embodiment 2

[0036] A kind of application of Trametes trichotis strain in the production of laccase by using municipal dewatered sludge, it comprises the following steps: (1) Liquid seed medium (g / L): glucose 20, soybean meal 5, yeast powder 1, dihydrogen phosphate Potassium 1, magnesium sulfate heptahydrate 0.5, initial pH 4, shaker speed 200 rpm, inoculated with Trametes trichosanthes SEM-6 (CCTCC No. M 2014002), and cultured for 60 hours to obtain liquid fermented seeds. (2) Liquid fermentation medium (g / L): dried sludge fermentation medium 50 (obtained from Jinan Everbright Water Plant No. The method is: add 1 part by weight of sodium hydroxide to municipal dewatered sludge with a water content of 100 parts and a water content of 80%, stir evenly and treat it at 35°C for 4 days to obtain the sludge fermentation substrate) , bran 10, potassium dihydrogen phosphate 1, magnesium sulfate heptahydrate 1.5, adjust the initial pH value to 7, and sterilize at 121°C for 30 minutes to obtain a l...

Embodiment 3

[0038] A kind of application of Trametes trichotis strain in the production of laccase by using municipal dewatered sludge, it comprises the following steps: (1) Liquid seed medium (g / L): glucose 20, soybean meal 5, yeast powder 1, dihydrogen phosphate Potassium 1, magnesium sulfate heptahydrate 0.5, initial pH 7, shaker speed 200 rpm, inoculated with Trametes trichosanthes SEM-6 (CCTCC No. M 2014002), and cultured for 72 hours to obtain liquid fermented seeds. (2) Liquid fermentation medium (g / L): dried sludge fermentation medium 40 (obtained from Jinan Everbright Water Plant No. The method is as follows: add 0.6 parts by weight of sodium hydroxide to municipal dewatered sludge with 100 parts by weight and 70% water content, stir evenly and treat at 30°C for 3 days to obtain the sludge fermentation substrate. ), potassium dihydrogen phosphate 3, magnesium sulfate heptahydrate 1, bran 15, adjust the initial pH value to 5, and sterilize at 121°C for 30 minutes to obtain a liqui...

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Abstract

The invention discloses trametes hirsute SEM-6 (trametes trogii SEM-6). The strain is preserved at the China Center for Type Culture Collection on January 6, 2014, and the preservation number is CCTCC N0.M2014002. Application of the trametes hirsute strain in production of laccase by using municipal dewatered sludge belongs to the technical field of bioengineering. The municipal dewatered sludge of which the organic matter content is 40-60% (according to dry weight) is taken as a main fermentation substrate to produce laccase in a liquid fermentation or solid fermentation manner. Thus, high-value utilization of an organic component in the sludge is achieved. The produced laccase can be applied to the aspects such as lignocellulose pretreatment, environmental pollutant treatment and the like.

Description

technical field [0001] The invention relates to a strain of Trametes trichotillotype and its application in producing laccase from municipal dewatered sludge. Background technique [0002] Laccase (EC 1.10.3.2) belongs to the cerulus oxidase family, and its substrate range is very wide, and it can oxidize monophenols, diphenols, polyphenols, diamines, aromatic amines, methylphenols, ascorbic acid and non-phenolic compounds Wait. Laccase is an environmentally friendly biocatalyst that can degrade substrates with the participation of oxygen, and its by-product is water. It is used in many fields such as environmental pollution control, bioremediation, paper industry, food industry, and biomass energy. , has a very broad application prospect. Laccase widely exists in organisms and has been found in fungi, bacteria, plants, animals and algae, among which the most capable of producing laccase is fungi, especially white rot fungi. [0003] In recent years, with the development ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/02C12R1/645
Inventor 梁晓辉张晓东华栋梁赵玉晓张杰司洪宇李岩许海朋牧辉
Owner ENERGY RES INST OF SHANDONG ACAD OF SCI
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