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Method for extracting trametes versicolor exopolysaccharide through two-membrane process

An extracellular polysaccharide, double-membrane technology, applied in the field of bioengineering, can solve the problems of production cost and product quality, consumption of alcohol and labor, low monosaccharide removal rate, etc. The effect of high removal rate of monosaccharides

Active Publication Date: 2012-12-19
JIANGSU SHENHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the alcohol precipitation process, since monosaccharides are often accompanied by polysaccharides, the removal rate of monosaccharides is low, so multiple alcohol precipitations are required, which consumes more alcohol and labor, and the production cost is higher; at the same time, the traditional thermal Concentration method consumes a lot of steam, which has a great impact on production cost and product quality

Method used

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  • Method for extracting trametes versicolor exopolysaccharide through two-membrane process

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Experimental program
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Effect test

Embodiment 1

[0025] The method for extracting the exopolysaccharide of Versicolor versicolor of the present invention is carried out according to the following steps:

[0026] 1. Source of Yunzhi fermentation broth: Trametes versicolor (Yunzhi), Trametes, preservation number CGMCC 5.161 provided by China General Microorganism Culture Collection and Management Center, the strain was transferred to slant medium for conventional culture at 26-28°C for 7 days, Get the activated Yunzhi bacterial strain; inoculate the activated Yunzhi bacterial strain on the seed medium, wherein the seed medium consists of 6% glucose, 4% yeast powder, 5% peptone, 0.2% potassium dihydrogen phosphate, and 0.02% magnesium sulfate , defoaming agent 0.1%, prepared with water, pH value is natural, 26 ~ 28 ℃ shaking culture for 3 days to obtain the first-class seed liquid of Versicolor versicolor; the first-class seed liquid is inserted into the second-level seed medium, and the second-level seed medium consists of 4% ...

Embodiment 2

[0032] The method for extracting the extracellular polysaccharide of Yunzhi of the present invention is carried out according to the following steps:

[0033]1. Microfiltration filtration: 2000Kg of Versicolor versicolor fermented liquid in Example 1 (solid content of fermented liquid is 3%) is filtered by microfiltration to obtain wet mycelia of 1730Kg and 270Kg of microfiltration dialyzate of Versicolor versicolor fermented liquid (the water content is 76%), the light transmittance of the dialysate is 86%, and the wet mycelium can be used to extract the intracellular sugar of Yunzhi. The membrane material used for microfiltration is a ceramic membrane with a pore size of 100nm. The specific process conditions are: the operating temperature is 50-60°C, the inlet pressure is 2.0bar, the outlet pressure is 1.0bar, and the pressure difference is 1.0bar.

[0034] 2, ultrafiltration filtration: the microfiltration dialyzate 1730Kg that step 1 gained is filtered through ultrafiltra...

Embodiment 3

[0038] The method for extracting the extracellular polysaccharide of Yunzhi of the present invention is carried out according to the following steps:

[0039] 1. Microfiltration filtration: 2000Kg of fermented Yunzhi fermented liquid (the solid content of the fermented liquid is 3%) is filtered through microfiltration to obtain 1790Kg of microfiltration dialyzate of Yunzhi fermented liquid and 210Kg of wet mycelium (containing The amount of water is 80%), the light transmittance of the dialysate is 80%, and the wet mycelium can be used to extract the intracellular sugar of Yunzhi. The membrane material used for microfiltration is a ceramic membrane with a pore size of 500nm. The specific process conditions are: the operating temperature is 50-60°C, the inlet pressure is 2.3bar, the outlet pressure is 0.9bar, and the pressure difference is 1.4bar.

[0040] 2, ultrafiltration filtration: the microfiltration dialysate 1790Kg obtained in step 1 is filtered by ultrafiltration to ob...

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Abstract

The invention discloses a method for extracting trametes versicolor exopolysaccharide through a two-membrane process, which comprises the steps that trametes versicolor fermentation liquor is sequentially subjected to microfiltration and ultrafiltration for separation to obtain trametes versicolor exopolysaccharide. According to the method, the conventional 'water extraction and alcohol precipitation' extraction mode of trametes versicolor exopolysaccharide is changed, the extraction effect of the active ingredients of the trametes versicolor exopolysaccharide is improved, the ethanol usage during the production is lowered, the filtrate wastewater discharge is reduced, and the economic and social benefits are obviously increased.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for extracting exopolysaccharides from fungi, more precisely, a method for extracting exopolysaccharides from versicolor versicolor based on membrane technology. Background technique [0002] Yunzhi intracellular glycopeptide, also known as Yunzhi glycopeptide, is composed of Yunzhi intracellular sugar and extracellular polysaccharide. It is a proteoglycan substance with physiological activity extracted and refined from Yunzhi fungus by modern bioengineering technology. Its functional ingredients The content is much higher than that of versicolor versicolor itself, and it is easy to be absorbed by the human body. [0003] The traditional production of Yunzhi exopolysaccharide is to concentrate the separated Yunzhi fermentation filtrate to a certain number of times, and then carry out multiple alcohol precipitations to remove the monosaccharides and inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 王文风徐国华袁兵兵徐玲张瑞华王英燕
Owner JIANGSU SHENHUA PHARMA
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