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Trametes versicolor capable of efficiently preprocessing lignin raw materials under alkaline condition and application thereof

A technology of Trametes versicolor and alkaline conditions, which can be applied to fungi, biofuels, waste fuels, etc., and can solve the problems of long cycle and low efficiency

Inactive Publication Date: 2015-04-29
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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Problems solved by technology

[0005] The use of basophilic microorganisms to pretreat lignocellulosic raw materials under mild alkaline conditions will be able to solve the problems of low efficiency and long cycle encountered in biological pretreatment.

Method used

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  • Trametes versicolor capable of efficiently preprocessing lignin raw materials under alkaline condition and application thereof
  • Trametes versicolor capable of efficiently preprocessing lignin raw materials under alkaline condition and application thereof
  • Trametes versicolor capable of efficiently preprocessing lignin raw materials under alkaline condition and application thereof

Examples

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Effect test

Embodiment 1

[0025] 1. Strain isolation

[0026] In Inner Mongolia, Ningxia and other places near saline-alkali land or saline-alkali lakes, collect soil samples around rotten wood at a distance of 10 cm from the surface, and put them in a 4°C refrigerator for later use.

[0027]Prepare 100 mL of alkaline liquid medium with bagasse as the sole carbon source, insert 1 g of the collected soil sample after sterilizing and cooling, and cultivate on a shaking table at 30°C and 150 rpm. After 5 days, take 1 mL of the cultured bacterial solution and transfer it to a fresh alkaline liquid medium with bagasse as the sole carbon source, and culture under the same conditions. Repeat this for 3-5 times. Then take the cultured bacterial solution and spread it on the alkaline solid medium containing bagasse as the only carbon source after gradient dilution, and culture it at 30°C for 3-5 days, observe the characteristics of the colony, and pick the colony with good growth and morphology Streak culture...

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Abstract

The invention discloses a new strain, namely Trametes versicolor T-4 capable of efficiently preprocessing lignin raw materials under the alkaline condition. The Trametes versicolor T-4 is preserved in the CGMCC (China General Microbiological Culture Collection Center) at Sep.2nd in 2014 with the preservation number of CGMCC No.9582, and the address of the CGMCC is Microbiology Research Institute of China Science Academy, No.3 of No.1 yard at Beichen west road of Chaoyang District in Beijing. The Trametes versicolor T-4, provided by the invention, can solve a series of problems that the biological preprocessing of the lignin raw materials is low in efficiency and long in cycle and the like, and has great potential in the aspects such as preparation of fuel ethanol and methane through the utilization of the lignin raw materials, and lays the foundation for the clean, efficient and low-cost preprocessing of the lignin raw materials.

Description

Technical field: [0001] The invention belongs to the technical field of environment and new energy, and specifically relates to a Trametes versicolor versicolor that can pretreat lignin raw materials under alkaline conditions and an application thereof. Background technique: [0002] Lignocellulose is a renewable resource and one of the most abundant organic matter on earth. my country is very rich in lignocellulosic raw materials. There are more than 700 million tons of crop straw alone every year. In addition to the huge amount of forestry fiber waste and industrial fiber waste, the total amount of lignocellulosic raw materials available every year can reach more than 2 billion tons. Therefore, lignocellulose has great potential application value in the fields of biomaterials and bioenergy. [0003] Lignocellulose is mainly composed of three components: cellulose, hemicellulose and lignin, of which lignin accounts for about 15%-30%. Lignin itself has a complex structure,...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/10C12P5/02C12R1/645
CPCC12P3/00C12P5/023C12P7/10C12P2203/00C12N1/145C12R2001/645Y02E50/10Y02E50/30
Inventor 袁振宏周桂雄庄新姝王闻亓伟谭雪松
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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