Water culture infinite propagation method for bamboo reed

Abstract

The invention provides a hydroponic infinite propagation method for bamboo reed. The method mainly comprises the following steps: S1, selection and pretreatment of lateral branches of bamboo reed: in March to May, cutting out the lateral branches with the length of 15-25cm by using sterile branch scissors, cleaning the lateral branches by using clear water, and culturing the cleaned lateral branches in an ABT rooting powder No.1 solution for 24h; s2, hydroponic treatment: putting the bamboo reed lateral branches treated by the rooting powder solution into a hydroponic container filled with clear water for hydroponic treatment; s3, carrying out plant division treatment, namely separating a primary tillering plant from a stock plant by using a sterile scalpel on a bamboo reed side branch with the primary tillering reaching 10-15cm; s4, stock plant transplanting and primary tillering plant water planting; s5, circulating operation: repeating the steps S3 and S4; and continuously utilizing the tillering plants to carry out hydroponic propagation until the required number of seedlings is reached. The method is simple to operate, low in cost, neat and strong in transplanted seedlings, high in propagation efficiency and capable of infinitely propagating, and can effectively meet the requirements of the market on the bamboo reed seedlings.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant asexual reproduction, and particularly relates to a method for unlimited hydroponic propagation of Arundo donax L.. Background Art

[0002] Arundo donax L. is a perennial tall tufted herb of the genus Arundo in the family Poaceae, with high biomass yield, strong ecological adaptability, drought resistance, barren tolerance, waterlogging tolerance, salinity tolerance and heavy metal tolerance. With the increasingly serious global energy crisis and environmental deterioration problems, Arundo donax L. has received more and more attention as a high-quality clean biomass energy plant, and the demand for Arundo donax L. seedlings is increasing.

[0003] The reproduction of Arundo donax L. mainly relies on asexual reproduction. The existing methods for propagating Arundo donax L. seedlings mainly include rhizome division, stem cutting, tissue culture, etc. For traditional seedling raising methods such as division and cutting, the propagation speed of seedlings is slow, the plant size and growth are inconsistent, far from meeting the requirements for seedlings in large-scale production; although tissue culture technology can obtain a large number of uniform seedlings, it has high requirements for production equipment and technology, the reserve of aseptic seedlings in the early stage is slow, the cycle is long, and the growth of tissue culture seedlings in the early stage after being taken out of the bottle is not as good as that of seedlings obtained by conventional methods, and it is difficult to meet the needs of large-scale rapid production and planting. Therefore, there is an urgent need for a method that can simply and quickly propagate Arundo donax L. seedlings. Summary of the Invention

[0004] The purpose of the present invention is to provide a method for unlimited hydroponic propagation of Arundo donax L. to solve the problems existing in the prior art.

[0005] To achieve the above purpose, the present invention provides the following technical solution: A method for unlimited hydroponic propagation of Arundo donax L., comprising the following steps:

[0006] S1. Selection and pretreatment of Arundo donax L. lateral branches: In March - May, select healthy Arundo donax L. with lateral branches and no pests and diseases, and use a sterile pruning shear to cut Arundo donax L. lateral branches with a length of 15 - 25 cm and located at 1.5 - 2 m of the Arundo donax L. stem. Appropriately prune the leaves on the intercepted lateral branches, remove the redundant leaves, and only retain 2 - 3 top leaves; rinse the lateral branches with clean water, and then place the Arundo donax L. lateral branches in a 100 mg / L solution of ABT rooting powder No. 1 for culture treatment for 24 h;

[0007] S2. Hydroponic treatment: Place the Phragmites australis lateral branches treated with ABT rooting powder No. 1 solution into a colorless transparent plastic cup with a height of 15 cm and a diameter of 10 cm, and provide a suitable environment with a light intensity of 15000-20000 Lux, a daylight duration of 16 hours, and a temperature of 25±2℃. The water level should be 3-5 cm above the base of the Phragmites australis lateral branches. Change the water every 3 days to keep the water clean. When the first tiller grows to 10-15 cm and the root length is more than 10 cm, proceed to the next step;

[0008] S3. Division treatment: Take out the side branches of Phragmites australis with a first-level tillering of 10-15 cm from the hydroponic container, separate the first-level tillering plant from the mother plant with a sterile scalpel, and ensure that the roots corresponding to the first-level tillering plant and the mother plant are also separated;

[0009] S4. Transplanting mother plants into seedlings and hydroponics of primary tillers: 8-10 cm thick substrate is placed at the bottom of the nutrient pot, the mother plant is placed in the nutrient pot, and then covered with 2-3 cm thick substrate, the roots of the mother plant are compacted with the substrate and then watered thoroughly with clean water; in terms of volume percentage, the substrate is a mixture of 75% nutrient soil and 25% vermiculite, and after the seedlings are hardened for 2 weeks, they are transplantable field seedlings; the primary tiller plant (new mother plant) is re-placed in a colorless transparent plastic cup with a height of 15 cm and a diameter of 10 cm, providing a suitable environment with a light intensity of 15000-20000 Lux, a daylight duration of 16 h, and a temperature of 25 ± 2 ° C, the water level requirement needs to be immersed 3-5 cm above the base of the side branches of Phragmites australis, and the clean water is replaced every 3 days to keep the water clean and promote the growth of secondary tillers (new primary tillers);

[0010] S5. Circular operation: repeat the above-mentioned steps of division treatment (S3), transplanting mother plants into seedlings and hydroponically cultivating primary tillers (S4), and continuously use the (mother plant) primary tillers for hydroponic propagation until the required number of seedlings is reached, and carry out annual cyclic reproduction.

[0011] Compared with the prior art, the invention has the following beneficial effects: the method for unlimited propagation of Phragmites australis hydroponics of the invention utilizes the hydroponics of Phragmites australis side branches, then transplants the mother plant and hydroponics the first-level tillering plant, and repeats the above operations to achieve the purpose of unlimited propagation of Phragmites australis seedlings. The method is simple to operate, low in cost, short in propagation cycle, high in propagation coefficient and seedling rate, high in seedling quality, uniform and strong in seedlings, and is an efficient method for unlimited propagation of Phragmites australis hydroponics. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 This is the rooting phenotype of the lateral branches of Phragmites australis in hydroponic culture;

[0013] Figure 2 Figure A shows the phenotype of the side branches of Phragmites australis before hydroponic division and Figure B shows the phenotype after division;

[0014] Figure 3 It is the phenotypic diagram of the seedlings formed after transplanting the mother plant of Arundo donax var. versicolor's lateral branches after hydroponics;

[0015] Figure 4 It is the phenotypic diagram of the rooting of the lateral branches of Arundo donax var. versicolor during hydroponics;

[0016] Figure 5 It is the diagram A of the lateral branches of Arundo donax var. versicolor before tillering during hydroponics and the phenotypic diagram B after tillering;

[0017] Figure 6 It is the phenotypic diagram of the seedlings formed after transplanting the mother plant of Arundo donax var. versicolor's lateral branches after hydroponics. Detailed implementation manners

[0018] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention.

[0019] A method for unlimited propagation of Arundo donax var. versicolor by hydroponics includes the following steps:

[0020] S1. Selection and pretreatment of Arundo donax var. versicolor's lateral branches: In March - May, select healthy Arundo donax var. versicolor with lateral branches and no pests and diseases, and use a sterile pruning shear to cut Arundo donax var. versicolor's lateral branches with a length of 15 - 25 cm and located at 1.5 - 2 m of the Arundo donax var. versicolor's stem. Appropriately trim the leaves on the cut lateral branches, remove the redundant leaves, and only retain 2 - 3 leaves at the top; rinse the lateral branches with clean water, and then place the Arundo donax var. versicolor's lateral branches in a solution of ABT rooting powder No. 1 with a concentration of 100 mg / L for culturing treatment for 24 h;

[0021] S2. Hydroponics treatment: Put the Arundo donax var. versicolor's lateral branches treated with the solution of ABT rooting powder No. 1 into a colorless transparent plastic cup with a height of 15 cm and a diameter of 10 cm, provide a suitable environment with a light intensity of 15000 - 20000 Lux, a daily light exposure time of 16 h, and a temperature of 25 ± 2 °C. The water level requirement is to submerge 3 - 5 cm above the base of the Arundo donax var. versicolor's lateral branches, and change the clean water every 3 days to keep the water quality clean. When the first - level tillers grow to 10 - 15 cm and the root length is more than 10 cm, proceed to the next step;

[0022] S3. Tillering treatment: Take out the Arundo donax var. versicolor's lateral branches with the first - level tillers reaching 10 - 15 cm from the hydroponics container, and use a sterile scalpel to separate the first - level tiller plants from the mother plant, ensuring that the roots corresponding to the first - level tiller plants and the mother plant are also separated;

[0023] S4. Transplanting the mother plant into seedlings and hydroponics of the first-level tillering plants: Fill the bottom of the nutrient bowl with 8 - 10 cm thick substrate, place the mother plant in the nutrient bowl, then cover it with 2 - 3 cm thick substrate, compact the roots of the mother plant with the substrate and pour it thoroughly with clean water; by volume percentage, the substrate is composed of 75% nutrient soil and 25% vermiculite. After acclimatizing the seedlings for 2 weeks of growth, it becomes the seedlings that can be transplanted into the field; put the first-level tillering plants (new mother plants) back into colorless transparent plastic cups with a height of 15 cm and a diameter of 10 cm, provide a suitable environment with a light intensity of 15000 - 20000 Lux, a daily light time of 16 h, and a temperature of 25 ± 2 °C. The water level requirement is to submerge 3 - 5 cm above the base of the Arundo donax lateral branches. Replace the clean water every 3 days to keep the water quality clean and promote the growth of the second-level tillering (new first-level tillering).

[0024] S5. Recycling operation: Repeat the above-mentioned tillering treatment (S3), transplanting the mother plant into seedlings and hydroponics of the first-level tillering plants (S4) steps, continuously use the first-level tillering plants (mother plants) for hydroponic propagation until the required number of seedlings is reached, and carry out annual cyclic propagation.

[0025] Example 1

[0026] S1. Selection and treatment of the lateral branches of Arundo donax var. versicolor: From March to May, select healthy Arundo donax var. versicolor with lateral branches without diseases and pests. Use a sterile pruning shear to cut 500 lateral branches of Arundo donax var. versicolor with lengths of 5 - 10 cm, 10 - 15 cm, 15 - 25 cm, and 25 - 30 cm respectively at the position 1.5 - 2 m from the stem of the Arundo donax. Appropriately prune the leaves on the cut lateral branches, remove the redundant leaves, and only retain 2 - 3 top leaves; rinse the lateral branches with clean water, and then place 100 lateral branches of Arundo donax var. versicolor in four length intervals into solutions with concentrations of 0 mg / L, 50 mg / L, 100 mg / L, 150 mg / L, and 200 mg / L of ABT rooting powder No. 1 solution for culturing treatment for 24 h;

[0027] S2. Hydroponic treatment: Put the lateral branches of Arundo donax var. versicolor treated with ABT rooting powder No. 1 solution into colorless transparent plastic cups with a height of 15 cm and a diameter of 10 cm, provide a suitable environment with a light intensity of 15000 - 20000 Lux, a daily light time of 16 h, and a temperature of 25 ± 2 °C. The water level needs to submerge 3 - 5 cm above the base of the lateral branches of Arundo donax var. versicolor. Replace the clean water every 3 days to keep the water quality clean and make it grow normally. Observe the rooting and seedling formation situation at 2 weeks, as shown in Table 1.

[0028] As shown in Table 1, it can be seen that when the concentration of ABT rooting powder is 100 mg / L and the length of the lateral branch is 15 - 25 cm, the rooting rate of Arundo donax var. versicolor is the highest and the growth is the best. Therefore, select Arundo donax var. versicolor with a concentration of ABT rooting powder of 100 mg / L and a lateral branch length of 15 - 25 cm for the later experiment, such asFigure 1 As shown, the hydroponic rooting phenotype of the lateral branches of Arundo donax var. versicolor with a length of 15 - 25 cm after being treated with ABT rooting powder at a concentration of 100 mg / L.

[0029] Table 1 Statistics of the number of rooted plants of the lateral branches of Arundo donax var. versicolor under different treatment conditions

[0030]

[0031] S3. Sub - clump treatment: Select 300 Arundo donax var. versicolor plants treated with ABT rooting powder solution at a concentration of 100 mg / L and with lateral branch lengths of 15 - 25 cm. When their primary tillers reach 5 - 10 cm, 10 - 15 cm, and 15 - 20 cm respectively, select 100 plants each time. Take the lateral branches of Arundo donax var. versicolor out of the hydroponic container, and use a sterile scalpel to separate the primary tiller plants from the mother plants. As Figure 2 shown, the mother plant is in the middle, and the primary tiller plants are on both sides;

[0032] S4. Transplanting of the mother plant and hydroponics of the primary tiller plants: Fill the bottom of the nutrient pot with a 8 - 10 cm thick substrate, place the mother plant in the nutrient pot, and then cover it with a 2 - 3 cm thick substrate. After compacting the substrate, pour it thoroughly with clean water. By volume percentage, the substrate is composed of 75% nutrient soil and 25% vermiculite. After 2 weeks of acclimatization and growth, it becomes a seedling that can be transplanted to the field, as Figure 3 shown, the seedlings of Arundo donax var. versicolor are neat and strong; Put the primary tiller plants (new mother plants) back into colorless transparent plastic cups with a height of 15 cm and a diameter of 10 cm, provide an appropriate environment with a light intensity of 15000 - 20000 Lux, a daily light exposure time of 16 h, and a temperature of 25 ± 2 °C. The water level needs to submerge 3 - 5 cm above the base of the lateral branches of Arundo donax var. versicolor. Replace the clean water every 3 days to keep the water quality clean and promote the growth of secondary tillers (new primary tillers). Observe the rooting and seedling formation of their secondary tillers (new primary tillers), see Table 2;

[0033] As shown in Table 2, it can be seen that when the concentration of ABT rooting powder is 100 mg / L and the length of the primary tiller (new mother plant) of Arundo donax var. versicolor is 10 - 15 cm, the number of rooted seedlings of the secondary tillers (new primary tillers) is the largest, the rooting and seedling formation rate is the highest, and the growth is the best. Therefore, select Arundo donax var. versicolor with a concentration of ABT rooting powder of 100 mg / L and a primary tiller length of 10 - 15 cm for subsequent sub - clump treatment experiments.

[0034] Table 2 Statistics of the rooting and seedling formation of secondary tillers after hydroponics of primary tillers with different lengths

[0035]

[0036] S5. Recycling operation: Repeat the above-mentioned tillering treatment in step S3 and the steps of transplanting the mother plant and hydroponically cultivating the first-level tillering plants, continuously using the tillering plants for hydroponic propagation until the required number of seedlings is reached. Other tillering plants can be reserved for propagation, and the annual cycle of reproduction can be carried out.

[0037] Among them, the Arundo donax var. versicolor in Example 1 is nonaploid Arundo donax var. versicolor.

[0038] Example 2

[0039] S1. Selection and treatment of the lateral branches of Arundo donax var. versicolor: In March to May, select healthy Arundo donax var. versicolor with lateral branches and no pests and diseases, and use a sterile pruning shear to cut the lateral branches of Arundo donax var. versicolor with a length of 15-25 cm and located at 1.5-2 m of the Arundo donax var. versicolor stem. Appropriately prune the leaves on the intercepted lateral branches, remove the redundant leaves, and only retain 2-3 leaves at the top; rinse the lateral branches with clean water, and then place the lateral branches of Arundo donax var. versicolor in a solution of ABT rooting powder No. 1 with a concentration of 100 mg / L for 24 h of culture treatment;

[0040] S2. Hydroponic treatment: Put the lateral branches of Arundo donax var. versicolor treated with the rooting powder solution into a colorless transparent plastic cup with a height of 15 cm and a diameter of 10 cm, provide a suitable environment with a light intensity of 15000-20000 Lux, a daily light time of 16 h, and a temperature of 25±2°C. The water level needs to submerge 3-5 cm above the base of the lateral branches of Arundo donax var. versicolor, and change the clear water every 3 days to keep the water quality clean. As Figure 4 shown, after treatment with ABT rooting powder at a concentration of 100 mg / L, the hydroponic rooting phenotype of the lateral branches of Arundo donax var. versicolor with a length of 15-25 cm. When the first-level tillering grows to 10-15 cm, proceed to the next step;

[0041] S3. Tillering treatment: Take out the Arundo donax var. versicolor lateral branches with the first-level tillering reaching 10-15 cm from the hydroponic container, and use a sterile scalpel to separate the first-level tillering plants from the mother plant. As Figure 5 shown, the mother plant is in the middle, and the first-level tillering plants are on both sides;

[0042] S4. Transplanting of the mother plant and hydroponic cultivation of the first-level tillering plants: Fill the bottom of the nursery pot with 8-10 cm thick substrate, place the mother plant in the nursery pot, and then cover it with 2-3 cm thick substrate. After compacting the substrate, pour it thoroughly with clean water; by volume percentage, the substrate is composed of 75% nutrient soil and 25% vermiculite. After 2 weeks of acclimatization and growth, it is the seedlings that can be transplanted to the field. As Figure 6As shown, the seedlings of Arundo donax var. versicolor are neat and strong. The first-level tillering plants (new mother plants) are re-placed into colorless transparent plastic cups with a height of 15 cm and a diameter of 10 cm, and provided with a suitable environment with a light intensity of 15,000 - 20,000 Lux, a daily light exposure time of 16 h, and a temperature of 25 ± 2 °C. The water level is required to submerge 3 - 5 cm above the base of the lateral branches of Arundo donax var. versicolor, and the clear water is changed every 3 days to keep the water quality clean and promote the growth of secondary tillering (new first-level tillering).

[0043] S5. Recycling operation: Repeat the above-mentioned tillering treatment step S3 and the steps of mother plant transplantation and water culture of tillering plants S4, and continuously use the tillering plants for hydroponic propagation until the required number of seedlings is reached.

[0044] Compare the hydroponic infinite propagation method of the lateral branches of Arundo donax var. arundinacea obtained in Example 1 with the existing propagation methods of other Arundo donax seedlings. The existing propagation methods of other Arundo donax seedlings are rhizome division propagation method, cutting propagation method and tissue culture method. Arundo donax is propagated according to the suitable natural material collection time and suitable propagation cycle of each method, and the comparison results are shown in Table 3:

[0045] Table 3 Comparison between other Arundo donax propagation methods and this method

[0046]

[0047] Note: n is for infinite propagation with a cycle of 2 - 3 months.

[0048] The above results show that compared with other Arundo donax propagation methods, the method of using the lateral branches of Arundo donax for hydroponic infinite propagation in the present invention has a short propagation cycle, high propagation coefficient and seedling formation rate, simple operation, high quality of the obtained seedlings, and neat and strong seedlings. In the present invention, only clear water is used for treatment. If the clear water is replaced with full-strength or diluted Hoagland nutrient solution, the effect is better in propagation and cultivation, which is not described in this invention patent.

[0049] In summary, the present invention uses the lateral branches of Arundo donax for hydroponics, then conducts mother plant transplantation and water culture of the first-level tillering plants (new mother plants), and repeats the above operations to achieve the purpose of infinite propagation of Arundo donax seedlings. This method is simple to operate, low in cost, short in propagation cycle, high in propagation coefficient and seedling formation rate, high in quality of seedlings, and neat and strong in seedlings, and is an efficient method for hydroponic infinite propagation of Arundo donax.

[0050] The above is only a preferred embodiment of the present invention and is not intended to limit the protection scope of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can still be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A method for the unlimited propagation of Arundo donax by hydroponics, characterized in that, The following steps are involved: S1. Selection and pretreatment of Phragmites australis lateral branches: In March-May, healthy, pest-free Phragmites australis with lateral branches were selected, and lateral branches with a length of 15-25 cm were cut with sterile branch shears; the leaves on the cut lateral branches were properly trimmed to remove excess leaves, leaving only 2-3 leaves at the top; the lateral branches were rinsed with clean water, and then the Phragmites australis lateral branches were placed in ABT rooting powder No. 1 solution for 24h; S2. Hydroponic treatment: Place the Phragmites australis lateral branches treated with the rooting powder solution into a hydroponic container filled with clean water, provide appropriate light and temperature, and replace the clean water every 3 days to keep the water clean; when the first tiller grows to 10-15 cm, proceed to the next step; S3. Division treatment: Take out the side branches of Phragmites australis with a first-level tillering of 10-15 cm from the hydroponic container, separate the first-level tillering plant from the mother plant with a sterile scalpel, and ensure that the roots corresponding to the first-level tillering plant and the mother plant are also separated; S4. Transplanting mother plants and hydroponically cultivating primary tillers: Place 8-10 cm thick substrate at the bottom of the nutrient pot, place the mother plant in the nutrient pot, and then cover it with 2-3 cm thick substrate. Compact the roots of the mother plant with the substrate and irrigate it thoroughly with clean water. In terms of volume percentage, the matrix is a mixture of 75% nutrient soil and 25% vermiculite. After the seedlings are hardened for 2 weeks, they are ready for transplanting into the field. The first-level tillering plants are placed back into the hydroponic container for hydroponic treatment to promote the growth of the second-level tillering plants. S5. Cyclic operation: repeat the above-mentioned steps of division treatment in S3, transplanting of mother plants and hydroponic cultivation of primary tillers in S4, and continuously use the primary tillers for hydroponic propagation until the required number of seedlings is reached.

2. The method for unlimited propagation of Arundo donax by hydroponics according to claim 1, wherein: In step S1, the position where the sterile branch shears cut the side branches is 1.5-2m from the Phragmites australis stem.

3. The method for infinite propagation of Arundo donax by hydroponics according to claim 2, characterized in that: The concentration of the ABT rooting powder No. 1 solution in step S1 is 100 mg / L, and the treatment time is 24 hours.

4. A method for unlimited propagation of Arundo donax by hydroponics according to claim 3, characterized in that: The hydroponic container in step S2 is characterized by a colorless transparent plastic cup with a height of 15 cm and a diameter of 10 cm.

5. A method for unlimited propagation of Arundo donax in hydroponics according to claim 4, characterized in that: In step S2, the light intensity is 15000-20000 Lux, the sunlight time is 16 hours, and the temperature is 25±2°C.

6. The method for unlimited propagation of Arundo donax by hydroponics according to claim 5, characterized in that: In step S2, the water level is required to submerge 3-5 cm above the base of the Phragmites australis lateral branches.

7. A method for unlimited propagation of Arundo donax by hydroponics according to claim 6, characterized in that: The node for the division treatment in step S3 is when the primary tiller of the Phyllostachys australis lateral branch reaches a height of 10-15 cm.

8. A method for unlimited propagation of Arundo donax in hydroponics according to any one of claims 1-7, characterized in that: In step S4, the matrix is a mixture of 75% nutrient soil and 25% vermiculite in terms of volume percentage.

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