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Aerobic denitrifying bacterium and application thereof in wastewater treatment

An aerobic denitrification and sewage treatment technology, applied in the field of environmental microorganisms, can solve the problems of low nitrogen removal efficiency and achieve a wide range of effects that are easy to cultivate

Active Publication Date: 2014-03-26
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the aerobic denitrifying bacteria that have been screened have low denitrification efficiency, and generally have denitrification activity when the dissolved oxygen concentration is low. It is of great significance to save the cost of denitrification and improve economic benefits in actual sewage treatment

Method used

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  • Aerobic denitrifying bacterium and application thereof in wastewater treatment
  • Aerobic denitrifying bacterium and application thereof in wastewater treatment
  • Aerobic denitrifying bacterium and application thereof in wastewater treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A kind of preparation method of aerobic denitrifying bacteria, its steps are:

[0050] Taking advantage of the characteristics of aerobic denitrification bacteria in denitrification under aerobic conditions, aerobic denitrification bacteria were enriched and isolated from constructed wetland soil by intermittent aeration method and acid-base indicator method.

[0051] (1) Enrichment

[0052] Under sterile conditions, weigh 10g of soil and put it into 500mL of liquid medium for enrichment, use intermittent aeration method for enrichment, the interval time is 12h, each aeration is 3h, cultivate in 30℃ incubator, every five days Transplant once, enrichment time is 1 month. The removal rate of nitrate nitrogen was measured to be 99.9%, and there was basically no accumulation of nitrite nitrogen.

[0053] The liquid medium is KNO 3 2g, K 2 HPO 4 1g, KH 2 PO 4 1g, MgSO 4 0.2g, sodium citrate 5g, trace salt solution 2mL; distilled water 1000mL.

[0054] (2) Primary sc...

Embodiment 2

[0061] Safety determination of Pseudomonas stutzeri JH-1:

[0062] Two treatments were set up in the experiment: control and JH-1 inoculation. Fill each tank with 10L of water, stock 20 zebrafish (approximately 2-3cm in length) of the same size, with an average weight of 0.5g per fish, and inject 250mL of JH-1 bacterial solution (the concentration of the bacterial solution is 9×105cfu·mL-1 ). The fish farming management is based on daily management, and the bacteria are injected once every 7 days, and the test period is 30 days.

[0063] During the test period, the fry ate normally, were very active, had no floating head phenomenon, and did not die in all treatments. The survival rate of the fish was 100%, which indicated that the JH-1 preparation was safe for fry.

Embodiment 3

[0065] Determination of the Treatment Ability of Pseudomonas stutzeri JH-1 to Natural Lake Water

[0066] The natural lake water used in the test was taken from the heavily polluted site of East Lake in Wuhan. The measured nitrate nitrogen concentration was 0.800mg / L and the pH was 6.0-6.5. The obtained natural lake water was grouped according to the following treatments: the first group, without adding sodium citrate and the isolated strain as a blank control; the second group, adding sodium citrate, without adding the isolated strain; the third group, without adding citric acid Sodium, but the isolate was added; the fourth group, sodium citrate, was also added to the isolate. Put 100mL sewage into a 250mL triangular flask, and inoculate 5mL of bacteria with a concentration of 9×10 5 cfu·mL-1 bacterial suspension, cultured on a shaker at 120 rpm at 30°C for 48 hours, and the supernatant was taken to detect the concentration of nitrate nitrogen, and each group was treated wit...

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Abstract

The invention discloses an aerobic denitrifying bacterium and application thereof in wastewater treatment. The applicant selects an aerobic denitrifying bacterium through enrichment, Pseudomonas stutzeri JH-1, CCTCC NO: M2013488 from a wet land. The bacterium can be used for treating wastewater with high NO3<->, reaches the highest removing rate of 99.6%, avoids the accumulation of nitrite nitrogen, meanwhile can remove COD in organic wastewater with the highest removing rate of 60%-80%; compared with other reported aerobic denitrifying bacteria for wastewater treatment, the bacterial strain provided by the invention can efficiently treating wastewater, achieves the removal rate of nitrate nitrogen of 99.6% after 24 h and the denitriding rate of 22.6 mg. L<-1>.h<-1>, which is 1.51-4.57 times of that of the reported bacterial strains, can be independently used or solidified for wastewater treatment, and is widely applied.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to an aerobic denitrification bacterium, and also relates to the application of the bacterium in sewage treatment. Background technique [0002] In recent years, the large-scale discharge of industrial wastewater and the large-scale application of chemical fertilizers in agriculture have led to increasingly prominent water environmental problems caused by nitrogen pollution, among which nitrate pollution is particularly serious. Nitrate and its derivatives affect human health due to their pathogenic and carcinogenic effects. Therefore, increasing the treatment of nitrogen pollution in sewage, especially improving the treatment efficiency of nitrate nitrogen is an urgent problem to be solved in the current sewage treatment technology. [0003] Biological denitrification denitrification is an economical and effective denitrification method using microorganisms ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N11/10C12N11/08C02F3/34C12R1/38
Inventor 吴振斌张雪琪张燕周巧红贺锋徐栋
Owner INST OF AQUATIC LIFE ACAD SINICA
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