Pseudomonas stutzeri and application thereof

A technology of Pseudomonas stutzeri and XL-2, which is applied in the field of environmental microorganisms and achieves the effect of wide application prospects

Inactive Publication Date: 2017-05-31
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, there are almost no reports on the auto-aggregation and sedimentation performance of aerobic denitrifying bacteria. Therefore, it is urgent to screen such bacteria from the environment and apply them in the field of nitrogen-containing wastewater treatment in order to obtain efficient denitrification. Simultaneously achieve efficient mud-water separation effect

Method used

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  • Pseudomonas stutzeri and application thereof
  • Pseudomonas stutzeri and application thereof
  • Pseudomonas stutzeri and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment one: the separation and identification of bacteria

[0026] 1. Isolation and purification of bacteria

[0027] Selective screening of bacteria: Inoculate 5 ml of the retrieved activated sludge into a conical flask filled with 100 ml of sterile water, and add a certain amount of glass beads to it to disperse the activated sludge. Then, the Erlenmeyer flask was placed on a constant temperature shaking shaker at 120 rpm for 1 h to obtain a uniformly dispersed bacterial suspension. Then pipette 1 ml of bacterial suspension into 100 ml of nitrate liquid medium, and acclimate at 120 rpm and 30 °C for 48 h. Then take the cultured bacterial suspension (1 ml) and transfer it to a nitrate liquid medium containing 100 mg / l for secondary selection acclimation, 120 rpm, 30 °C constant temperature acclimatization for 48 h. After three times of selective culture, the bacterial culture solution was inoculated into LB medium for enrichment and culture for 12-24 h.

[0028]...

Embodiment 2

[0039] Embodiment two: Pseudomonas stutzeri strain XL-2 to NO in wastewater 3 - -N, TN, COD removal effect

[0040] Pseudomonas stutzeri strain XL-2 NO 3 - -N is the only nitrogen source, CH 3 When COONa is the only carbon source, the NO 3 - -N, TN, COD degradation and NO 2 - -Accumulation of N. The initial COD concentration was 2971 mg / l, the initial TN was 103 mg / l, and the initial NO 3 - -N concentration was 89 mg / l. Continuously cultivated in a shaker at 30 °C and 120 rpm for 72 h, and samples were taken at regular intervals. For detailed results, see figure 2 and image 3 . In the first 20 h, the strain was in the logarithmic growth phase and grew rapidly; at 20 h, the strain OD 600 (The absorbance value of the solution at a wavelength of 600 nm) was 0.761; after 36 h, the bacterial growth reached a stationary phase, OD 600 Stable around 0.9. Strain XL-2 to NO within 20 h 3 - - The removal rate of N is as high as 95.5%, the removal rate of TN is 84.8%,...

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Abstract

The invention discloses a pseudomonas stutzeri and application thereof. The name of pseudomonas stutzeri is pseudomonas stutzeri XL-2 and is preserved in China General Microbiological Culture Collection Center (CGMCC for short) on November 10, 2016; the preservation number is No. 13252. The strain is obtained by taking active sludge of Chongqing Jiguanshi Sewage Treatment Plant as the raw material and carrying out repeated domestication separation by using a high-concentration nitrate nitrogen NO3-N selective culture medium. The pseudomonas stutzeri is capable of carrying out aerobic denitrification by using high-concentration NO3-N under aerobic condition; after the pseudomonas stutzeri is cultured for 20 hours, the removal rate of NO3-N of the pseudomonas stutzeri reaches up to 95.5%; the removal rate of total nitrogen (TN) can reach 84.8%; the final removal rate of COD reaches 74.5%; and the accumulation of an intermediate product, namely nitrite nitrogen (NO2-N), is avoided. In addition, the pseudomonas stutzeri has the effect of automatically gathering and settling; rod-shaped cells are embedded with each other and are gathered at the bottom of the culture medium in bulks; sludge-water separation can be rapidly achieved; the pseudomonas stutzeri has a good application prospect in the field of treatment of high-concentration nitrogen-containing wastewater.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a strain of Pseudomonas stutzeri and its application. Background technique [0002] Nitrogen in water mainly exists in the form of organic nitrogen, ammonia nitrogen and nitrate nitrogen, and is one of the most common pollutants that cause eutrophication of water bodies, endanger human health and destroy the ecological environment of water bodies. The discharge of agricultural nitrogen fertilizers, industrial wastewater and urban sewage is the main source of nitrogen in water bodies. How to remove nitrogen pollution in water economically and efficiently has become a research focus and hotspot in the field of water pollution control. Microbes have the characteristics of wide source, fast reproduction and strong adaptability to the environment, so biological denitrification is considered to be the most economical and effective method. [0003] Tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/02C02F3/34C12R1/38
CPCC02F3/02C02F3/34C12N1/205C12R2001/38Y02W10/10
Inventor 赵彬安强田梦程丹阳谭攀黄源生
Owner CHONGQING UNIV
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