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Swamp Rhodopseudomonas of using nitrite nitrogen in high effect, and application

A technology of swamp rhodopseudomonas and nitrous nitrogen, which is applied in the field of photosynthetic bacteria and can solve problems such as effects affecting the application.

Inactive Publication Date: 2007-10-10
珠海市农科中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is worth noting that the strains required in aquaculture production practice not only need to have the potential to eliminate nitrite nitrogen, but also need to be able to reproduce rapidly in water, otherwise it is difficult to use in production practice, because aquaculture water not only contains various A rich organic matter, also contains various types of microorganisms, especially including a large number of plankton that feed on bacteria, the presence of these microorganisms will affect the effect of the application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation of embodiment 1 bacterial classification

[0023] Take 100 μl of Rhodopseudomonas palustris 2-8 bacteria solution, spread it on the plate containing medium 2, place it in an anaerobic tank, and cultivate it for 3 days under the condition of light intensity of 2000lux and temperature of 28℃~30℃ , pick 1 inoculation loop strain from the culture medium slant and inoculate it into liquid medium 1, and anaerobically culture it under the same temperature and light conditions for 3 days, then re-amplify twice in liquid medium 1 at an inoculum concentration of 3%, That is, the strain culture fluid that can be used for large-scale production is obtained.

[0024] A, the formula of medium 1 is as follows: ammonium chloride 0.1%, sodium hydrogen phosphate 0.05%, magnesium sulfate or magnesium chloride 0.02%, sodium chloride 0.2%, yeast extract or peptone 0.1~0.2%, the above ingredients are dissolved in water, Steam sterilization at 121°C for 20 minutes. The follo...

Embodiment 2

[0026] The large-scale production culture of embodiment 2 bacterium

[0027] Disinfect culture containers and tap water with sodium hypochlorite solution, and then neutralize with sodium thiosulfate. Then, in the culture container, add reagents according to any culture medium formula described in the content of the invention (2), be prepared into a culture medium, insert 10% of the three-level bacterial classification, and finally neutralize with a neutralizing agent after disinfection Fill the whole container with tap water, place it at 28°C to 30°C, and cultivate it for 5 to 7 days under a light intensity of 2000 lux to obtain the product.

[0028] Disinfection treatment reagents and time are shown in Table 1:

[0029] disinfection pair

Embodiment 3

[0030] The rejuvenation of embodiment 3 bacterial strains

[0031] Collect 200-300g of fertile and fresh sediment from shrimp ponds or fish ponds, put them in a 500ml salt water bottle, and add 10g of Penaeus vannamei feed, then fill up the salt water bottle with water from fish ponds or shrimp ponds, after autoclaving and cooling, press 3% of the inoculum amount is inserted into the strain to be rejuvenated, and anaerobic light culture is carried out for 2 to 6 days to obtain a strain with high activity of reducing nitrite nitrogen. If necessary, the above operation can be repeated more than three times. After the rejuvenation of the strain, the nitrite nitrogen assimilation ability of the rejuvenated strain was measured according to the evaluation method introduced in Example 6.

[0032] After rejuvenating the patent bacterial strain 2-8 according to the above-mentioned method, measure through the evaluation method of embodiment 6, the result is as follows:

[0033] ...

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PUM

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Abstract

This invention relates to a kind of photosynthetic bacterium that has a high assimilation utility to nitrite nitrogen. More specifically, this invention provides a Rhodopseudomonas palustris strain that can assimilate nitrite nitrogen, and its r ejuvenation, preservation, biological detection and application. The photosynthetic bacterium is a kind of biological water purifier and biological fertilizer, and can be used in controlling the content of nitrite nitrogen in water body for aquaculture, improve environmental pollution, and eliminate cinnamic acid and benzoic acid. The photosynthetic bacterium can be used as nitrogen-fixing biological fertilizer and cucumber growth promoter.

Description

technical field [0001] The invention relates to a method capable of efficiently utilizing nitrite nitrogen (in the form of NO 2 - Nitrogen-containing compounds that exist in the form of salts and salts) photosynthetic bacteria, specifically providing a Rhodopseudomonas palustris 2-8 strain (CCTCC) that can efficiently assimilate and reduce nitrite nitrogen M 205147) and its industrialized cultivation method, strain preservation method, strain rejuvenation method and bioassay method for nitrite nitrogen elimination. It belongs to the category of biological water purifiers and biological fertilizers. Background technique [0002] Residual bait, fish and shrimp excrement, dead biological residues, drugs, and fertilizers in aquaculture water accumulate, decompose, and transform at the bottom of the water body, forming hydrogen sulfide, ammonia nitrogen, nitrite nitrogen, and other nitrogen oxides that are harmful to fish. Shrimp has toxic compounds that deteriorate water qual...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12Q1/04C05F11/08A62D3/00C12R1/38
CPCY02W30/40
Inventor 喻国辉陈远凤陈燕红杨鹏杨紫红程萍
Owner 珠海市农科中心有限公司
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