Pseudomonas stutzeri strain and application thereof in degrading polycyclic aromatic hydrocarbon with high molecular weight

A Pseudomonas stutzeri, high molecular weight technology, applied in bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of limited repair effect and low removal efficiency of high molecular weight PAHs

Inactive Publication Date: 2009-12-16
SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Affected by the lack of high-molecular-weight PAHs-degrading bacteria, the remediation effect of existing microbial remediation technologies is often limited to the removal of low-molecular-weight PAHs in the environment, and the removal efficiency of high-molecular-weight PAHs is extremely low

Method used

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  • Pseudomonas stutzeri strain and application thereof in degrading polycyclic aromatic hydrocarbon with high molecular weight
  • Pseudomonas stutzeri strain and application thereof in degrading polycyclic aromatic hydrocarbon with high molecular weight

Examples

Experimental program
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Effect test

Embodiment 1

[0013] The preparation of embodiment 1 Pseudomonas stutzeri MT-5

[0014] The composition of SMS culture medium (g / L) is: (NH 4 ) 2 SO 4 0.5, NaNO 3 0.5, CaCl 2 0.02, MgSO 4 0.2, KH 2 PO 4 1.0, NaH 2 PO 4 .H 2 O 1.0, pH=7.5, chemical reagents were purchased from Guangzhou Chemical Reagent Factory, all were analytically pure.

[0015] (1) Enrichment culture

[0016] Use the sediment sampler to take samples from the sediment of the Zhongda Wharf, put the sediment into a sterile sampling bottle, and send it to the laboratory. Store at 4°C for later use. Take 10 g of bottom mud sample and make it into 20% suspension, and inoculate it into SMS liquid medium containing phenanthrene (0.05g / L) with 10% inoculum size. Constant temperature 30 ℃, shaker culture for seven days. Take 1% of the bacterial solution and insert it into the same culture medium, and continue to culture on a shaking table, repeating this many times until an enriched culture with significant degr...

Embodiment 2

[0037] The preparation of embodiment 2 Pseudomonas stutzeri MT-5 bacterial suspension

[0038] Pseudomonas stutzeri MT-5 was inoculated in SMS culture medium and cultured at 30°C until logarithmic growth phase. After collecting the bacteria by centrifugation, a certain concentration of bacterial suspension was prepared, and the absorbance value of the suspension at 600 nm was OD600=0.68.

Embodiment 3

[0039] Example 3 Degradation of BAP / BGP by Pseudomonas stutzeri MT-5

[0040] BAP and BGP were purchased from Fisher Reagent Company in the United States with a purity of 99%; SPME micro-extraction tips were purchased from SUPELCO (100 μm) Company.

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Abstract

The invention relates to a novel strain of bacteria and application thereof in degrading polycyclic aromatic hydrocarbon with high molecular weight. The strain is identified and named as Pseudomonas stutzeri MT-5 strain which has bacteriological characteristic of Gram-negative, has cells in a straight or bent rod shape but not spiral shape and not in a chain, has the size of 0.60-0.8*1.4-2.0mu m, has polar flagellum, can move, does not form spore or have sheath or protrusion, has no poly beta hydroxybutyrate observed, has round bacterial colony, not irregular edge, rough surface and irregular bump shape, and is white and opaque. The Pseudomonas stutzeri MT-5 strain can be applied to degrading PAHs, BAP / BGP with high molecular weight. The Pseudomonas stutzeri MT-5 strain has the degradation efficiency of 65 percent on the BGP and 59.6 percent on the BAP in 168h, and has the degradation capacity higher than related bacteria for degrading the BAP / BGP reported at home and abroad.

Description

technical field [0001] The invention relates to a new bacterial strain and the application of the bacterial strain in degrading macromolecule polycyclic aromatic hydrocarbons. Background technique [0002] Microbial remediation of polycyclic aromatic hydrocarbons (PAHs) pollution refers to the process of using PAHs-degrading bacteria to degrade PAHs pollutants in the environment or convert PAHs into harmless substances. It has the advantages of high efficiency, low consumption, and environmental friendliness. The core of this technology is to obtain microbial strains that can efficiently degrade PAHs, because the quality of the strains directly affects the restoration time and effect. So far, among the numerous PAHs-degrading bacteria that have been isolated and identified, most strains can only degrade low-molecular-weight PAHs, while very few strains have the ability to degrade tetracyclic and higher-molecular-weight PAHs. Affected by the lack of high-molecular-weight PAH...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/38A62D101/20
Inventor 郑政伟徐亚幸李开明朱芳
Owner SOUTH CHINA INST OF ENVIRONMENTAL SCI MEP
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