90 results about "Poly-beta-hydroxybutyrate" patented technology

The invention discloses a method for preparing a composite board capable of simultaneously slowly releasing nitrogen and adsorbing/biologically degrading petroleum hydrocarbons. The method comprising the steps of weighing urea aqueous solution and formaldehyde aqueous solution in the proportion of 6.5:1 and regulating the pH value of the urea aqueous solution to 7.2-7.4; adding formaldehyde into the urea aqueous solution, stirring, regulating the pH value to 3.2-3.4 and adding deionized water to obtain a mixed solution M1; mixing poly(beta-hydroxybutyrate) and chloroform solution of polyethylene glycol in the proportion of 5:1; dropwise adding the M1 into the mixed chloroform solution in the proportion of 1:4; spraying distilled water into a container filled with liquid nitrogen to manufacture ice ball particles, screening, and putting in a mould to compact; pouring the mixed solution into the mould, carrying out freezing shaping and obtaining a composite board after demoulding and freezing; and culturing the composite board in a petroleum hydrocarbon degrading bacteria solution for 24h, drying in the air and obtaining the composite board capable of simultaneously slowly releasing nitrogen and adsorbing/biologically degrading petroleum hydrocarbons. The method disclosed by the invention has the advantages that the manufacturing process is simple; and the obtained composite board is high in petroleum hydrocarbon pollutant treating efficiency and incapable of causing secondary pollution and damage to the water ecological environment.

The invention discloses a porous particle with functions of adsorbing and biodegrading petroleum hydrocarbon and a preparation method of the porous particle. Poly-beta-hydroxybutyrate chloroformic solution with mass fraction of 8% and polyoxyethylene chloroformic solution with mass fraction of 8% are mixed according to the proportion of 4:1; deionized water is dripped into the mixed solution according to the proportion of 1:4, and mixture of the deionized water and the mixed solution is stirred for 4 minutes; obtained mixed liquid is dripped into polyvinyl alcohol solution with mass fraction of 0.3% according to the proportion of 1:1, and mixture is stirred for 4 minutes; polyvinyl alcohol solution with mass fraction of 0.4% is added in the mixture according to the proportion of 2:1, the mixture is centrifugally separated after being stirred for 7 hours to 8 hours, and is washed by deionized water for three times, the porous particle is obtained after the mixture is lyophilized; and the porous particle is taken out to be aired after being cultivated in petroleum hydrocarbon degrading bacterium culture solution for 24 hours. The porous particle and the preparation method thereof are simple in process, a prepared porous particle is high in efficiency to treat pollutants of the petroleum hydrocarbon, and an ecological environment of water cannot be polluted secondarily or damaged.

The invention discloses a degradable paper plastic composite bag including a plastic layer and a paper layer, the plastic layer is arranged on the surface of the paper layer, the plastic layer includes starch, vegetable fibre, polyvinyl alcohol, poly Beta-hydroxybutyrate, polyethylene, polycarbonate, nanometer titanium dioxide, glycerin, a plasticizer, and a stabilizing agent. The degradable paper plastic composite bag has excellent photodegradation and biodegradation properties, and is mild in degradation conditions and short in degradation time. The nanometer titanium dioxide in the plastic layer further improves the ultraviolet resistance capability of the plastic layer on the basis of having excellent degradability. In addition, the preparation method of the degradable paper plastic composite bag is simple in step and the degradable paper plastic composite bag can be produced industrially in batch because the raw materials are easy to get.

The invention discloses a biodegradable perforated plate for adsorption and biodegradation of petroleum hydrocarbon pollutants as well as its preparation method. The method comprises the steps of: spraying distilled water into a container loaded with liquid nitrogen for rapid refrigeration so as to prepare spheroidal ice ball particles, which are sieved by a sieve and then placed in a mould cavity for compaction; mixing 10 mass percent of a poly-beta-hydroxybutyrate chloroform solution and 10 mass percent of a polyoxyethylene chloroform solution fully in a volume ratio of 5:1, cooling the mixture, and then pouring it into the mould loaded with ice ball particles for freezing molding, then carrying out demoulding and vacuum freeze drying for removing chloroform and ice ball particles, thusobtaining a composite perforated plate of poly-beta-hydroxybutyrate and polyoxyethylene; culturing the perforated plate in a culture solution of petroleum hydrocarbon degrading bacteria for 24h, thentaking it out for air drying, thus obtaining a biodegradable perforated plate for adsorption and biodegradation of petroleum hydrocarbon pollutants. The preparation method of the invention has a simple process, and the prepared perforated plate has high treatment efficiency to petroleum hydrocarbon pollutants, and cause no secondary pollution and destruction to a water ecological environment.

The invention discloses a preparation method of a biodegradable oil absorption microsphere with a nitrogen nutrient element slow release function. The preparation method comprises the following steps of: preparing a urea solution and a formalin solution according to the ratio of 6.5:1, and regulating the pH value of the urea solution to be 7.2-7.4; adding formalin into the urea solution, stirring for 90-100 minutes, regulating the pH value to be 3.2-3.4, and adding deionized water so as to obtain a mixed liquid M; mixing chloroform solutions of beta polyhydroxybutyrate and polyethylene glycol both with the mass percentage of 7.5% according to the ratio of 5:1; dropping M1 into the chloroform mixed solution according to the ratio of 1:4; dropping the obtained mixed solution into a polyving akohol solution with the mass percentage of 3.5% according to the ratio of 1:1; and dropping the polyving akohol solution with the mass percentage of 0.5% into the mixed liquid according to the ratio of 2:1, stirring for 7-8 hours, separating, freezing and drying so as to obtain the microsphere. According to the invention, the preparation technology is simple; the obtained microsphere has high treatment efficiency to petroleum hydrocarbon pollutants and does not generate secondary pollution and damage to a water body.

The invention provides a high-intensity starch-based degradation environmental protection material and a manufacturing method thereof, which relates to a rapid degradation raw material used for manufacturing disposable tableware and disposable wrappage and the manufacturing method thereof. The present disposable foaming plastic tableware has the disadvantages of hard heat insulation, complicated process line, high production cost, undesirable production quality and white pollution. The components of the product include macromolecular natural starch, polyvinyl alcohol, plant sugar, polyvinyl alcohol, a degradation control agent, a multipolymer of polycaprolactone and poly-beta-hydroxybutyrate and hexa-butyl ester hexa-butyl glycan; wherein, the starch is 51-80 weight portions, the polyvinyl alcohol is 12-20 weight portions, the plant sugar is 2-10.5 weight portions, plasticizer is 2.5-8 weight portions, the degradation control agent is 0.2-1 weight portions, the multipolymer of polycaprolactone and poly-beta-hydroxybutyrate is 5-9 weight portions and the hexa-butyl ester hexa-butyl glycan is 0.2-0.5 weight portions. The high-intensity starch-based degradation environmental protection material of the invention is used for manufacturing the disposable tableware and the disposable warppage.

The invention discloses a porous adsorption plate with multiple functions on petroleum hydrocarbon polluted water bodies and a preparation method thereof. The preparation method comprises the following steps: preparing a NH4HCO3 solution, a NH4H2PO4 solution, a Ca(NO3)2 solution, a NH4OH solution, a hydrochloric acid solution, a NH4Cl water solution and a calcium potassium ethylenediamine tetraacetate solution into a mixed solution F; preparing urea and formalin into a mixed solution L; preparing a poly-beta-hydroxy-butyrate chloroformic solution and a polyethyleneglycol chloroformic solution into a mixed solution M; dropwisely adding the mixed solution L into the mixed solution M to obtain a mixed solution N; dropwisely adding the mixed solution F into the mixed solution N to obtain a mixed solution O; injecting distilled water into a container filled with liquid nitrogen to prepare ice granules, screening, and compacting in a mold; and casting the mixed solution O into the mold to perform freeze molding, demolding and refrigerating to obtain the porous adsorption plate with multiple functions on petroleum hydrocarbon polluted water bodies. The manufacturing technique is simple; and the prepared adsorption plate has high treatment efficiency for petroleum hydrocarbon pollutants, and can not bring about secondary pollution and damage onto the ecological environment of the water bodies.

The invention relates to a bacillus megaterium Bm-10 strain for highly producing poly-beta- hydroxybutyrate (PHB) and application thereof, belonging to the field of biotechnological medicine and living. The preservation number of the bacillus megaterium Bm-10 strain for highly producing the poly-beta-hydroxybutyrate is CCTCC No: M209131. The bacillus megaterium Bm-10 strain for highly producing the poly-beta-hydroxybutyrate is obtained from sludge by screening. The stain can be used for the microbiological preparation of PHB and solves the problems of low yield, high cost, and the like of degradable plastics strains produced by the microbiological method in the prior art.

The invention discloses a degradable lunch box, which is prepared from the following raw materials by weight: 40-50 parts of poly beta-hydroxybutyrate, 20-30 parts of modified polybutylene succinate,20-30 parts of composite of biochar, 10-20 parts of lignocellulose aerogel, 10-20 parts of polylactic acid, 5-10 parts of modified hydroxypropyl acetylated corn starch, and 5-8 parts of a coupling agent. According to the present invention, the degradable lunch box has characteristics of low production cost, good overall strength and good temperature resistance, and can be rapidly degraded in the natural environment.

The invention discloses a method for co-producing butanedioic acid and PHB by utilizing colon bacillus for the first time, which is used for knocking out sdhAB and iclR genes in the colon bacillus, and for constructing aerobic fermentation way of the butanedioic acid, so that butanedioic acid fermentation technology bacterial strains MG1655 delta sdhAB delta iclR::kan; on that basis, a PHB biosynthetic pathway is educed into colon bacillus MG1655 delta sdhAB delta iclR::kan, thereby successfully constructing the butanedioic acid and PHB fermentation way in the same colon bacillus. The fermentation and test results prove that the engineering bacterial strain can effectively transform glucose to generate the butanedioic acid and the PHB. 75g/L of glucose is consumed, and 37.5g/L of butanedioic acid is generated; at the same time, PHB is accumulated by thalli to be weighed as 28% of the dry weight of the thalli. The engineering bacterial strain has important application prospect.

The invention discloses a biological sewage treatment device and a biological sewage treatment method for synchronous nitrogen and phosphorus removal and sludge reduction. The device comprises a water inlet pipe 1, an anaerobic pond 2, a mid-sedimentation tank 3, an intermediate pool 4, a BAF 5, an anoxic pond 6, a post-aeration tank 7, a secondary sedimentation tank 8, and a water outlet pipe 9 in series connection in order; the treatment process comprises the following steps: mixing raw water and P-rich returned sludge in the anaerobic pond, wherein denitrifying phosphorus accumulating bacteria (DPBs) absorb and store VFA in a body in a form of PHB (poly-beta-hydroxybutyrate) and release a large amount of phosphorus; removing VOD remained in the BAF and nitrating the nitrogen; introducing BAF nitrification liquid and the sludge in the mid-sedimentation tank into the anoxic pond together, wherein the DPBs performs denitrification for absorbing the phosphorous by using the PHB in the body as an energy source and a carbon source and using NO3<-> as an electron acceptor; removing the unnecessary phosphorus contained in the post-aeration tank and blowing off the nitrogen to prevent the sludge from floating upwards; discharging the supernatant in the secondary sedimentation tank, wherein part of the sludge containing a large amount of DPBs is refluxed and the remaining sludge is discharged. The biological sewage treatment device and the biological sewage treatment method for the synchronous nitrogen and the phosphorus removal and the sludge reduction resolves the conflict between the denitrification and the phosphorous removal, and ensures the stable water quality of effluent and low yield of the sludge.

The invention discloses a sustained-release microsphere to achieve the aims of contraception and long-term treatment of endometriosis uterine, which comprises the following components: (1) nomegestrol acetate or analogs thereof, and (2) a biodegradable polymer, wherein the weight ratio of an ester-terminated polymer to a carboxyl-terminated polymer is between 1:0.01-1:1; the ester-terminated polymer is selected from polylactide-glycolide, polylactide, poly-beta-hydroxybutyrate, poly ortho ester, polycaprolactone, poly-phosphate, polyanhydride, or poly-cyanoacrylate; the carboxyl-terminated polymer is selected from polyglycolic acid and polylactic acid-glycolic acid; and the weight of the nomegestrol acetate or the analogs thereof accounts for 5 to 80 percent of the total weight of the microsphere. The invention also discloses a preparation method and application of the sustained-release microsphere.

The invention discloses a main cis-acting element of a shrimp white spot syndrome virus (WSSV) iel promoter and a transcription factor combined with the same and application. In the invention, by starting from transcriptional regulation of WSSV iel and carrying out structural and functional analysis on the promoter of the WSSV iel through deletion and mutation, a 12-bp DNA is found to be the maincis-acting element of the WSSV iel and is a crucial factor for the high expression of the iel. In the invention, a DNA affinity chromatography method is used for purifying a protein combined with a DNA segment from a nucleus protein Sf9, the protein is identified to be PHB2 (Poly-Beta-Hydroxybutyrate 2) through biological mass spectrometry, and the interaction between the protein and the DNA segment is proved to be specific by an electrophoretic mobility shift assay. Experiment results prove that PHB2 serves as a transcription factor and is specifically combined with a 12-bp DNA sequence of the iel promoter to start WSSV immediate early gene transcription so as to further regulate the replication of the WSSV, and can be used as an effective action target of medicaments for screening medicaments for resisting the shrimp WSSV.

The invention provides a sewage treatment technology and particularly relates to a technology for treating low-carbon/nitrogen-ratio sewage on the basis of dominant culture of high-concentration heterotrophic endogenous denitrifying bacteria and autotrophic nitrifying bacteria populations. Specially arranged sedimentation tanks are adopted to prevent dominant growth of heterotrophic microorganisms relative to autotrophic nitrifying bacteria after the heterotrophic microorganisms enter aerobic tanks, so that the autotrophic nitrifying bacteria keep high concentration and high activity, and high-efficiency ammonia-nitrogen nitrification is realized. With adoption of an alternate water inlet and outlet mode, the heterotrophic microorganisms in anaerobic tanks/anoxic tanks convert organic carbon sources into intracellular PHB (poly-beta-hydroxybutyrate) in a first half cycle, intracellular PHB is taken as an electron donor for denitrification in a second half cycle, the limited carbon sources in the sewage are used to the greatest extent for denitrification and are prevented from entering the aerobic tanks and being subjected to oxidation consumption, ammonia nitrogen in the low-carbon/nitrogen-ratio sewage is removed effectively under the condition that an external carbon source is not needed, synchronous nitrification and denitrification are realized, the dephosphorization effect is good, and the technology has good application prospect.

The invention discloses a preparation method of a biodegradable oil absorption plate capable of slowly releasing nitrogen element. The method comprises the following steps of measuring urea and formaldehyde aqueous solutions in a ratio of 6.5 to 1, and regulating the pH value of the urea aqueous solution to be between 7.2 and 7.4; adding formaldehyde into the urea aqueous solution, stirring for 90 to 100 minutes, regulating the pH value to be between 3.2 and 3.4, and adding deionized water to obtain mixed solution M1; mixing 9.5 percent by mass of poly-beta-polyhydroxybutyrate and polyethyleneglycol chloroform solutions in a ratio of 5 to 1; dropwise adding the M1 into the chloroform mixed solution in a ratio of 1 to 4; injecting the distilled water into a container filled with liquid nitrogen, quickly freezing to manufacture spherical ice ball particles, screening and compacting in the cavity of a die; pouring the mixed solution into the die for chilling, demolding and lyophilizing in vacuum to obtain the biodegradable oil absorption plate capable of slowly releasing nitrogen element. The preparation method is simple in manufacture process, and the prepared porous plate is high in petroleum hydrocarbon pollutant processing efficiency, and cannot cause secondary pollution or destruction to the water ecological environment.

The invention discloses a degradable foaming package material. The degradable foaming package material comprises, by weight, 40-75 parts of poly-beta-hydroxybutyrate, 8-16 parts of poly(butanediol succinate), 12-42 parts of modified plant fibers, 1-4 parts of foaming agents, 2-4 parts of inorganic powder and 1-6 parts of fusion agents. The modified plant fibers are obtained by drying plant fibers under 2-5MPa at the temperature of 220-240 DEG C under the protection of inert gases to achieve the water content of 3-5% and subjecting the plant fibers to 5-10 minutes of electromagnetic radiation under the protection of the inert gases after smashing. The degradable foaming package material has the advantages of high tensile strength, high elongation at break, high impact resistance, biodegradability, non-existence of environment pollution and the like, thereby being capable of replacing polyolefins-type foaming package materials.

The invention discloses an adsorption plate with high-efficient adsorption and enhanced biodegradation of petroleum hydrocarbon pollutants and a preparation method thereof. The preparation method comprises the steps of preparing a mixed solution F from NH4HCO3, Ca(NO3)2, (NH4)2HPO4, a urea water solution and an ethylenediaminetetraacetic acid calcium sodium solution; preparing a mixed solution L from urea and a formaldehyde water solution; preparing a mixed solution M from a poly beta-hydroxybutyrate chloroform solution and a polyethylene glycol chloroform solution; dropping the mixed solution L into the mixed solution M to obtain a mixed solution N; dropping the mixed solution F into the mixed solution N to obtain a mixed solution O; spraying distilled water into a container containing liquid nitrogen to prepare ice ball particles, screening and then placing in a mold for compaction; pouring the mixed solution O into the mold for freezing and shaping, then demolding, and freezing to obtain the adsorption plate; culturing the adsorption plate in a petroleum hydrocarbon degrading bacterial culture solution for 24h and then taking out for air-drying. The preparation process disclosed by the invention is simple, and the prepared adsorption plate has high treatment efficiency against petroleum hydrocarbon pollutants and can prevent secondary pollution and destruction to an ecological environment of a water body.

The invention discloses a copolymer adsorption ball with an enhanced biodegradation function against various organic pollutants and a preparation process thereof. The preparation process comprises the steps of preparing a mixed solution F from NH4HCO3, Ca(NO3)2, (NH4)2HPO4, a urea water solution and an ethylenediaminetetraacetic acid calcium sodium solution; preparing a mixed solution L from urea and a formaldehyde water solution; preparing a mixed solution M from a poly beta-hydroxybutyrate chloroform solution and a polyethylene glycol chloroform solution; dropping the mixed solution L into the mixed solution M to obtain a mixed solution N; dropping the mixed solution F into the mixed solution N to obtain a mixed solution O; dropping the mixed solution O into a polyvinyl alcohol solution to obtain a mixed solution P; adding the polyvinyl alcohol solution into the mixed solution P and performing centrifugal separation to obtain the copolymer adsorption ball with a nitrogen and phosphorus sustained release function; culturing the copolymer adsorption ball in a petroleum hydrocarbon degrading bacterial culture solution for 24h and then taking out for air-drying. The preparation process disclosed by the invention is simple, and the prepared copolymer adsorption ball has the nitrogen and phosphorus sustained release function, the function of adsorbing various organic pollutants and the function of degrading petroleum hydrocarbons.