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Bacillus megaterium Bm-10 strain for highly producing poly-beta-hydroxybutyrate as well as screening method and application thereof

A technology of Bacillus megaterium and screening method, applied in the field of Bacillus megaterium Bm-10 strain, can solve the problems of high cost and low yield of degradable plastic strains

Active Publication Date: 2010-12-22
克拉玛依市新奥达石油技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the increasing demand for degradable plastics in the market, but the production of degradable plastics by microbial methods in the prior art is low in yield and high in cost.

Method used

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  • Bacillus megaterium Bm-10 strain for highly producing poly-beta-hydroxybutyrate as well as screening method and application thereof
  • Bacillus megaterium Bm-10 strain for highly producing poly-beta-hydroxybutyrate as well as screening method and application thereof
  • Bacillus megaterium Bm-10 strain for highly producing poly-beta-hydroxybutyrate as well as screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Isolation of Bacillus megaterium Bm-10 strain with high PHB production from sludge

[0019] (1) culture medium

[0020] Beef extract peptone medium: beef extract (20g), peptone (5g), sodium chloride (5g), water (1L), pH 7.2.

[0021] (2) Method

[0022] A gradient dilution separation method was used. Take 0.2g of soil, add 5mL of distilled water, heat at 85°C for 15min, and carry out gradient dilution (10 -1 ~10 -4 ), choose the appropriate concentration (10 -2 ) 100 μL coated beef extract peptone medium plate, cultivated at 30°C for 24 hours, round milky white colonies appeared. Pick a single colony, after further separation and purification, the obtained bacterial strain is qualitatively determined by Sudan black staining method to PHB ( figure 1 D). Select the number of the strain containing PHB and store it in a refrigerator at 4°C for future use. The slant of the beef extract peptone test tube was used to activate and subculture the strain, and th...

Embodiment 2

[0024] Example 2 The method in which Bacillus megaterium (B.megaterium) Bm-10 strain is used for the preparation of PHB

[0025] (1) Strains:

[0026] Bacillus megaterium (B. megaterium) Bm-10 strain, the preservation number of the strain is: CCTCC NO: M209131.

[0027] (2) Method

[0028] Pick an appropriate amount of bacteria from the slant medium of the activated beef extract peptone test tube and insert it into the beef extract peptone culture solution, activate and cultivate at 30°C for 24 hours; put 50mL beef extract peptone culture solution into a 250mL triangular flask, and take the seeds cultivated for 24 hours liquid, after inoculation by 8% inoculum size, 30 DEG C, 200r / min, shaking flask culture 96h, can obtain the Bacillus megaterium thallus (as image 3 shown).

[0029] PHB extraction and quantitative analysis: the culture solution obtained from the shake flask was centrifuged at 10000r / min for 5min to collect the bacteria. After washing 3 times with sterile ...

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PUM

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Abstract

The invention relates to a bacillus megaterium Bm-10 strain for highly producing poly-beta- hydroxybutyrate (PHB) and application thereof, belonging to the field of biotechnological medicine and living. The preservation number of the bacillus megaterium Bm-10 strain for highly producing the poly-beta-hydroxybutyrate is CCTCC No: M209131. The bacillus megaterium Bm-10 strain for highly producing the poly-beta-hydroxybutyrate is obtained from sludge by screening. The stain can be used for the microbiological preparation of PHB and solves the problems of low yield, high cost, and the like of degradable plastics strains produced by the microbiological method in the prior art.

Description

technical field [0001] The invention belongs to the fields of biotechnology, medicine and life, and in particular relates to a Bacillus megaterium Bm-10 strain with high yield of poly-β-hydroxybutyrate (PHB) and its application. Background technique [0002] Poly-β-hydroxybutyric acid (PHB) is a kind of biological polyester in cells, its mechanical properties are similar to some thermoplastic materials such as polypropylene, and can be completely degraded by microorganisms, so it is called "biodegradable plastics" , is a real environment-friendly product. Researching biodegradable plastics to replace chemically synthesized plastics can help us solve the problem of plastic pollution, save energy, and achieve sustainable development of resources and the environment. PHB is a polymer storage of carbon source and energy (molecular weight 50,000-1,000,000) synthesized by microorganisms under the condition of nutritional imbalance such as carbon and nitrogen. As a degradable mat...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C12P7/62C12R1/11
Inventor 蒋冬花周琴
Owner 克拉玛依市新奥达石油技术服务有限公司
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