Pseudomonas frederiksbergensis sp. NT-2 and application thereof

A Pseudomonas, unit technology, applied in the direction of application, bacteria, and other chemical processes, can solve the problems of soil organic matter content decline, unfavorable crop growth, negative effects, etc., to increase soil organic matter content, alleviate toxicity, and reduce biological effect of effectiveness

Active Publication Date: 2016-01-20
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to improve soil acidification and increase crop yield, lime is widely used, but there are many restrictions on the application of lime, a

Method used

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  • Pseudomonas frederiksbergensis sp. NT-2 and application thereof
  • Pseudomonas frederiksbergensis sp. NT-2 and application thereof
  • Pseudomonas frederiksbergensis sp. NT-2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Isolation, Identification and Characterization of Pseudomonas (Pseudomonasfrederiksbergensissp.) NT-2

[0037] 1.1 Test soil

[0038] The soil collected from apple orchards in Yantai City after spraying with Bordeaux mixture. Fresh soil samples were passed through a 2mm nylon sieve and stored in a dark place in a refrigerator at 4°C.

[0039] 1.2 Medium

[0040]Beef extract peptone solid medium: beef extract 3.0g / L, peptone 10.0g / L, NaCl 5.0g / L, distilled water 1000mL, agar 20g / L, pH7.0.

[0041] Beef extract peptone liquid medium: beef extract 3.0g / L, peptone 10.0g / L, NaCl 5.0g / L, distilled water 1000mL, pH7.0.

[0042] Screening medium: Beef extract peptone medium is prepared by adding copper sulfate stock solution.

[0043] 1.3 Screening of copper-resistant microorganisms in soil

[0044] Weigh 5.0g of soil sample, put it into a Erlenmeyer flask containing 45mL of sterile water (put in a little glass beads), shake at constant temperature for more than...

Embodiment 2

[0050] Embodiment 2: the improvement effect of acidic soil amendment to orchard soil

[0051] 1 test soil

[0052] The soil for testing was collected from the apple orchard in Haiyang City, Yantai, Shandong Province. When collecting the soil, the surface soil was removed, air-dried, passed through a 2mm nylon sieve, and mixed evenly. The soil pH was 5.13.

[0053] 2 Materials and methods

[0054] 2.1 Preparation of microbial agent

[0055] 1) Slant culture: inoculate Pseudomonas frederiksbergensissp. NT-2 on the slant medium, and culture in a constant temperature incubator at 28°C for 36-48h;

[0056] 2) Shaker culture: Inoculate the bacteria cultured in the above step 1) into beef extract-peptone liquid medium, shake and culture in a shaker at pH6.8-7.2, 28°C, 180r / min for 36h to logarithmic growth phase;

[0057] 3) Fermentation tank culture: Inoculate the bacterial solution cultivated in step 2) into a fermenter equipped with beef extract peptone medium at a 2% inoculum...

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Abstract

The invention relates to the field of microorganism soil conditioning and remediation, and in particular relates to pseudomonas frederiksbergensis sp. NT-2 and an application thereof as an acid soil conditioner. The strain is already preserved in a preservation institute appointed by the State Intellectual Property Office in November 5, 2015, the name of the preservation institute is the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.11585. The soil conditioner provided by the invention can adjust the pH value of soil, promote formation of soil aggregate, improve physical properties of soil, and promote growth of plants, and contains organic matters, calcium, magnesium, silicon and microelements which are beneficial for growth of plants; the added microorganism bacterial agent is relatively good in heavy metal resistance and growth promoting property, toxicity of heavy metals to plants can be reduced, the micro-ecological environment of soil can be improved, the mineral nutrition can be improved, and plant growth can be promoted. The soil conditioner provided by the invention is relatively good in acid soil conditioning effect, can improve soil fertility, and has a relatively good application prospect in biological remediation of heavy metal polluted soil.

Description

technical field [0001] The invention relates to the field of microbial soil conditioning and restoration, in particular to a pseudomonas (Pseudomonasfrederiksbergensissp.) NT-2 and its application as an acidic soil improver. Background technique [0002] Soil acidification refers to the process of increasing hydrogen ions in the soil or the process of soil acidity changing from low to high. It is a continuous natural process. Soil acidification will directly lead to the deterioration of soil physical and chemical properties, and break the original soil ecological environment conditions suitable for crop growth, so that the pH of the soil will drop, resulting in a decrease in the content of available boron and molybdenum in the soil, while the available iron, aluminum, and manganese in the soil The increase of the content of ions will cause manganese and aluminum poisoning in crops, which in turn will lead to the loss of calcium, magnesium and other elements in the soil, resu...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/00C12R1/38C09K109/00
Inventor 涂晨刘颖李连祯章海波骆永明
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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