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Pseudomonas stutzeri and culture application thereof

A technology of Pseudomonas stutzeri and liquid culture medium, which is applied in the field of environmental microorganisms, can solve the problems that Pseudomonas stutzeri does not have salt tolerance, is not suitable for high-salt wastewater treatment, and has a complicated preparation process, and achieves Improve wastewater treatment effect, maintain growth characteristics and tolerance characteristics, and achieve good treatment effect

Active Publication Date: 2017-05-10
CHINA PETROLEUM & CHEM CORP +1
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AI Technical Summary

Problems solved by technology

This method requires the cultivation of multiple bacterial strains for compounding to prepare salt-tolerant bacterial agents, the preparation process is complicated, and the production cost is relatively high.
[0010] At present, it is known that the screened Pseudomonas stutzeri has problems such as no salt tolerance or poor salt tolerance, and is not suitable for the treatment of high-salt wastewater

Method used

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  • Pseudomonas stutzeri and culture application thereof
  • Pseudomonas stutzeri and culture application thereof

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Experimental program
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Embodiment 2

[0031] Embodiment 2 screening and separation and purification

[0032] The activated sludge obtained from a petrochemical enterprise in Yueyang City, Hunan Province for the treatment of epichlorohydrin production wastewater was domesticated and cultivated for 15 days by gradually increasing the salt concentration in the culture medium.

[0033] The above-mentioned domesticated activated sludge was taken, and the method of dilution coating was used for preliminary separation in the enrichment medium, thereby obtaining 15 colonies with good growth status and different characters. The main components of the enrichment medium used are: 1000mg / L COD, 1000mg / L calcium ion concentration, 10000mg / L total salt, and 2.0% agar content in the solid medium used.

[0034] Then, the obtained colony was transferred by streaking on a plate, and transferred to a screening medium by streaking for further separation and screening. The main components of the screening medium used are: COD Cr The...

Embodiment 3

[0036] The investigation of embodiment 3 Pseudomonas stutzeri FSTB-5 salt tolerance

[0037] The culture method of Pseudomonas stutzeri FSTB-5 of the present invention comprises: bacterial strain activation, liquid seed liquid culture, aeration culture, concrete process is as follows:

[0038] (1) Strain activation: Pseudomonas stutzeri FSTB-5 was inoculated on a plate containing FSTB solid medium, cultured at 35°C for 48 hours, and then stored in a refrigerator at 4°C until use. The FSTB solid medium is: FeSO 4 •7H 2 O 25mg / L, NH 4 NO 3 286mg / L, KCl 929mg / L, CaCl 2 2769mg / L, NaCl 21008mg / L, beef extract 5g / L, peptone 10g / L, agar 20g / L, pH 7.8.

[0039] (2) Liquid seed liquid culture: Prepare FSTB liquid medium, pack it into Erlenmeyer flasks, sterilize and cool to room temperature, use an inoculation loop to pick the activated strains in the slope and inoculate them into the Erlenmeyer flasks under a sterile environment. Incubate at 35°C for 48 hours. The FSTB liquid...

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Abstract

The invention relates to pseudomonas stutzeri and a culture application thereof. The strain is pseudomonas stutzeri FSTB-5, collected at 'China General Microbiological Culture Collection Center' on June 1, 2015, and the preservation number is CGMCC No. 10940. The strain can be applied to efficiently removing COD (chemical oxygen demand) in salty wastewater with salt content of 1.0wt%-10wt%, and can grow at the high temperature of 40 DEG C. The invention further provides COD removing salt-tolerant bacteria which comprise the pseudomonas stutzeri FSTB-5. The COD in the high-salt wastewater can be rapidly degraded by the pseudomonas stutzeri, and the pseudomonas stutzeri has the advantages of high salt resistance, good treatment effect and the like.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a salt-tolerant deCOD Pseudomonas stutzeri and its cultivation application. The bacterial strain is especially suitable for efficient removal of COD in high-salt wastewater. Background technique [0002] Highly saline wastewater with a salt content higher than 1% from petroleum, chemical, pharmaceutical, and fertilizer industries generally contains high COD, and the continuous discharge of pollutants in wastewater into the environment will cause serious pollution problems. The shortage of fresh water resources is becoming more and more serious, and the treatment and recycling of waste water have been paid more attention by people. [0003] Physical or chemical treatment of COD in high-saline wastewater is costly and may cause secondary pollution. Traditional biological methods have great advantages in treating low-salinity wastewater, but in high-salt, hig...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 郭志华高会杰孙丹凤赵胜楠李宝忠
Owner CHINA PETROLEUM & CHEM CORP
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