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A kind of Kitami microbacterium and its cultivation application

A technology of microbacteria and liquid culture medium, which is applied in the field of environmental microorganisms, can solve the problems of microbacteria Kitami that have not been screened for high-salt wastewater, high production cost, complicated preparation process, etc., and achieves improved wastewater treatment effect and salt tolerance. Strong ability and good processing effect

Active Publication Date: 2019-09-10
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method requires the cultivation of multiple bacterial strains for compounding to prepare salt-tolerant bacterial agents, the preparation process is complicated, and the production cost is relatively high.
[0006] At present, there is no report on the screening of Microbacteria kitami for the treatment of high-salt wastewater

Method used

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  • A kind of Kitami microbacterium and its cultivation application
  • A kind of Kitami microbacterium and its cultivation application
  • A kind of Kitami microbacterium and its cultivation application

Examples

Experimental program
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Effect test

Embodiment 2

[0026] Embodiment 2 screening and separation and purification

[0027] The activated sludge obtained from a petrochemical enterprise in Yueyang City, Hunan Province for the treatment of epichlorohydrin production wastewater was domesticated and cultivated for 15 days by gradually increasing the salt concentration of the culture solution.

[0028] The above-mentioned domesticated activated sludge was taken, and the diluted coating method was used for preliminary separation in the enriched medium, thus obtaining 15 colonies with good growth status and different characters. The main components of the enrichment medium used are: 1000mg / L COD, 1000mg / L calcium ion concentration, 10000mg / L total salt, and 2.0% agar content in the solid medium used.

[0029] Then, the obtained colony was transferred by streaking on a plate, and transferred to a screening medium by streaking for further separation and screening. The main components of the screening medium used are: 1000mg / L COD, 4000...

Embodiment 3

[0031] Example 3 Investigation of Microbacterium Kitami FSTB-4 Salt Tolerance

[0032] The cultivation method of Kitami microbacterium FSTB-4 of the present invention comprises: bacterial strain activation, liquid seed solution cultivation, aeration cultivation, concrete process is as follows:

[0033] (1) Strain activation: Microbacterium kitami FSTB-4 was inoculated into a plate containing FSTB solid medium, cultured at 30°C for 48 hours, and then stored in a 4°C refrigerator for use. The FSTB solid medium is: FeSO 4 •7H 2 O 25mg / L, NH 4 NO3 286mg / L, KCl 929mg / L, CaCl 2 2769mg / L, NaCl 21008mg / L, beef extract 5g / L, peptone 10g / L, agar 20g / L, pH 7.8.

[0034] (2) Liquid seed liquid culture: Prepare FSTB liquid medium, divide it into Erlenmeyer flasks, sterilize and cool to room temperature, use an inoculation loop to pick the activated strains on the plate and inoculate them into Erlenmeyer flasks under a sterile environment. Incubate at 30°C for 48 hours. The FSTB liq...

Embodiment 4

[0041] Example 4 Kitami microbacterium FSTB-4 open culture

[0042] The open culture method of Microbacterium Kitami FSTB-4 of the present invention comprises: strain activation, liquid seed liquid culture, open aeration culture, pure strain detection and dominant bacterium maintenance, and specific process is as follows:

[0043] (1) Strain activation: Microbacterium kitami FSTB-4 was inoculated into a plate containing FSTB solid medium, cultured at 30°C for 48 hours, and then stored in a 4°C refrigerator for use. The FSTB solid medium is: FeSO 4 •7H 2 O 25mg / L, NH 4 NO 3 286mg / L, KCl 929mg / L, CaCl 2 2769mg / L, NaCl 21008mg / L, beef extract 5g / L, peptone 10g / L, agar 20g / L, pH 7.8.

[0044] (2) Liquid seed liquid culture: Prepare FSTB liquid medium, divide it into Erlenmeyer flasks, sterilize and cool to room temperature, use an inoculation loop to pick the activated strains on the plate and inoculate them into Erlenmeyer flasks under a sterile environment. Incubate at 3...

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Abstract

The invention relates to microbacterium kitamiense and culture application thereof. The strain is microbacterium kitamiense FSTB-4 and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 1, 2015; and the preservation number is CGMCC No.10939. The strain can be applied to efficient removal of COD (Chemical Oxygen Demand) in salt-containing wastewater with the salt content of 1.0wt%-5.0wt%. The invention further provides a salt-tolerant bacterium agent for removing the COD and the bacterium agent comprises the microbacterium kitamiense FSTB-4. The microbacterium kitamiense provided by the invention can be used for rapidly degrading and removing the COD in the high-salt-content wastewater and has the characteristics of high salt-tolerant capability, good treatment effect and the like.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a salt-tolerant de-COD microbacterium kitami and its cultivation application. The strain is especially suitable for efficient removal of COD in high-salt wastewater. Background technique [0002] Highly saline wastewater with a salt content higher than 1% from petroleum, chemical, pharmaceutical, and fertilizer industries generally contains high COD, and the continuous discharge of pollutants in wastewater into the environment will cause serious pollution problems. The shortage of fresh water resources is becoming more and more serious, and the treatment and recycling of waste water have been paid more attention by people. [0003] Physical or chemical treatment of COD in high-saline wastewater is costly and may cause secondary pollution. Traditional biological methods have great advantages in treating low-salinity wastewater, but in high-salt, high-acid-b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 郭志华高会杰孙丹凤赵胜楠李宝忠
Owner CHINA PETROLEUM & CHEM CORP
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