Microbacterium kitamiense and culture application thereof

A technology of microbacteria and liquid culture medium, applied in the field of environmental microorganisms, can solve the problems of complicated preparation process, no screening of high-salt wastewater with microbacteria Kitami, high production cost, and achieve good treatment effect and improve wastewater treatment effect. , The effect of strong salt tolerance

Active Publication Date: 2017-05-10
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method requires the cultivation of multiple bacterial strains for compounding to prepare salt-tolerant bacterial agents, the preparation process is complicated

Method used

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  • Microbacterium kitamiense and culture application thereof
  • Microbacterium kitamiense and culture application thereof
  • Microbacterium kitamiense and culture application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0026] Example 2 Screening and separation and purification

[0027] The activated sludge taken from a petrochemical enterprise in Yueyang City, Hunan Province for treating epichlorohydrin production wastewater was domesticated and cultivated for 15 days by gradually increasing the salt concentration of the culture solution.

[0028] The above-mentioned acclimated activated sludge was taken and preliminarily separated in the enriched medium by the dilution coating method, thereby obtaining 15 colonies with good growth status and different characters. The main components contained in the enrichment medium used are: COD of 1000 mg / L, calcium ion concentration of 1000 mg / L, total salt content of 10000 mg / L, and agar content of 2.0% in the solid medium used.

[0029] Then, the obtained colonies were transferred by plate streaking method, and then streaked and transferred to the screening medium for further separation and screening. The main components of the screening medium used ...

Embodiment 3

[0031] Example 3 Investigation on the salt tolerance of Microbacterium Kitamii FSTB-4

[0032] The method for culturing Microbacterium Kitamii FSTB-4 of the present invention comprises: bacterial activation, liquid seed liquid culture, and aeration culture, and the specific process is as follows:

[0033](1) Strain activation: Microbacter Kitamii FSTB-4 was inoculated into a plate containing FSTB solid medium, cultured at 30°C for 48 hours, and then stored in a 4°C refrigerator for later use. Described FSTB solid medium is: FeSO 4 •7H 2 O 25mg / L, NH 4 NO 3 286mg / L, KCl 929mg / L, CaCl 2 2769mg / L, NaCl 21008mg / L, beef extract 5g / L, peptone 10g / L, agar 20g / L, pH 7.8.

[0034] (2) Liquid seed liquid culture: prepare FSTB liquid medium, distribute it in triangular flasks, sterilize and cool to room temperature, pick the activated strains in the plate with an inoculation loop in a sterile environment and inoculate them into the triangular flasks. Incubate at 30°C for 48 hours...

Embodiment 4

[0041] Example 4 Open culture of Microbacterium Kitamii FSTB-4

[0042] The open culture method of Microbacterium Kitamii FSTB-4 of the present invention includes: strain activation, liquid seed liquid culture, open aeration culture, pure strain detection and dominant bacteria maintenance, and the specific process is as follows:

[0043] (1) Strain activation: Microbacter Kitamii FSTB-4 was inoculated into a plate containing FSTB solid medium, cultured at 30°C for 48 hours, and then stored in a 4°C refrigerator for later use. Described FSTB solid medium is: FeSO 4 •7H 2 O 25mg / L, NH 4 NO 3 286mg / L, KCl 929mg / L, CaCl 2 2769mg / L, NaCl 21008mg / L, beef extract 5g / L, peptone 10g / L, agar 20g / L, pH 7.8.

[0044] (2) Liquid seed liquid culture: prepare FSTB liquid medium, distribute it in triangular flasks, sterilize and cool to room temperature, pick the activated strains in the plate with an inoculation loop in a sterile environment and inoculate them into the triangular flas...

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Abstract

The invention relates to microbacterium kitamiense and culture application thereof. The strain is microbacterium kitamiense FSTB-4 and is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 1, 2015; and the preservation number is CGMCC No.10939. The strain can be applied to efficient removal of COD (Chemical Oxygen Demand) in salt-containing wastewater with the salt content of 1.0wt%-5.0wt%. The invention further provides a salt-tolerant bacterium agent for removing the COD and the bacterium agent comprises the microbacterium kitamiense FSTB-4. The microbacterium kitamiense provided by the invention can be used for rapidly degrading and removing the COD in the high-salt-content wastewater and has the characteristics of high salt-tolerant capability, good treatment effect and the like.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a salt-tolerant COD-removing Microbacterium Kitamie and its cultivation application, and the strain is particularly suitable for the efficient removal of COD in high-salt wastewater. Background technique [0002] High-salt wastewater with salt content higher than 1% from industries such as petroleum, chemical, pharmaceutical and fertilizer generally contains high COD, and the continuous discharge of pollutants in wastewater into the environment will cause serious pollution problems. The shortage of fresh water resources is becoming more and more serious, and the treatment and recycling of waste water has attracted more attention. [0003] Physical or chemical treatment of COD in highly saline wastewater is costly and may cause secondary pollution. The traditional biological method has great advantages in the treatment of low-salinity wastewater, but in the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 郭志华高会杰孙丹凤赵胜楠李宝忠
Owner CHINA PETROLEUM & CHEM CORP
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