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A kind of open culture method and application of paracoccus fstb-2

A FSTB-2, open culture technology, applied in the field of environmental microorganisms, can solve the problems of unclean treatment, affect the treatment effect, troublesome filtration methods, etc., and achieve the effect of reducing the culture cost, good treatment effect and strong salt tolerance.

Active Publication Date: 2020-03-17
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the filtration method is cumbersome and the treatment is not clean; the acidification method kills miscellaneous bacteria while the growth of the dominant bacteria itself is also inhibited; the way of adding antibiotics also has a killing effect on the dominant bacteria, which in turn affects the treatment effect

Method used

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  • A kind of open culture method and application of paracoccus fstb-2

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The open culture method of paracoccus FSTB-2 of the present invention comprises five stages such as bacterial strain activation, liquid seed liquid culture, aeration culture, pure bacterial strain detection and dominant bacterial strain maintenance, and specific process is as follows:

[0025] (1) Strain activation: Paracoccus FSTB-2 was inoculated into a plate containing FSTB solid medium, cultured at 35°C for 48 hours, and then stored in a refrigerator at 4°C until use. The FSTB solid medium is: FeSO 4 •7H 2 O 25mg / L, NH 4 NO 3 286mg / L, KCl 929mg / L, CaCl 2 2769mg / L, NaCl 21008mg / L, beef extract 5g / L, peptone 10g / L, agar 20g / L, pH 7.8.

[0026] (2) Liquid seed liquid culture: Prepare FSTB liquid medium, divide it into Erlenmeyer flasks, sterilize and cool to room temperature, use an inoculation loop to pick the activated strains on the plate and inoculate them into Erlenmeyer flasks under a sterile environment. Incubate at 35°C for 48 hours. The FSTB liquid med...

Embodiment 2

[0031] The treatment process and operating conditions are the same as in Example 1, the difference is that in step (4) add rifamycin SV at a concentration of 5mg / L, and continue to add 5 culture cycles, and the floating microorganisms are drained and discharged Time to discharge, after which the normal open culture operation process is resumed. Collect the above cultured bacterial solution, spread it on a plate containing FSTB solid medium after dilution, count the grown colonies and count the percentage of colonies similar to FSTB-2.

Embodiment 3

[0033] The treatment process and operating conditions are the same as in Example 1, the difference is that in step (4), add nalidixic acid at a concentration of 30mg / L, and continue to add 5 culture cycles, and the floating microorganisms will be discharged during drainage and discharge , and then resume the normal open culture operation process. Collect the above cultured bacterial solution, spread it on a plate containing FSTB solid medium after dilution, count the grown colonies and count the percentage of colonies similar to FSTB-2.

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Abstract

The present invention relates to an opening type culture method of Paracoccus FSTB-2, wherein a bactericide is poured during the opening type culture process of Paracoccus FSTB-2, the Paracoccus sp. FSTB-2 is preserved in the China General Microbiological Culture Collection Center on June 1, 2015 and has a preservation number of CGMCC No.10938, and the bactericide is one or a plurality of materials selected from lincomycin, rifamycin SV, nalidixic acid, guanidine hydrochloride, and the like. According to the present invention, according to the bacterium characteristic of Paracoccus FSTB-2, the specific bactericide is poured during the opening type culture process of Paracoccus FSTB-2 by using the salt-containing and COD-containing wastewater, such that the bacterial contamination can be significantly reduced, and the Paracoccus FSTB-2 growth and reproduction and the wastewater treatment effect cannot be affected.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to an open culture method and application of paracoccus FSTB-2. Background technique [0002] Highly saline wastewater with a salt content higher than 1% from petroleum, chemical, pharmaceutical, and fertilizer industries generally contains high COD, and the continuous discharge of pollutants in wastewater into the environment will cause serious pollution problems. The shortage of fresh water resources is becoming more and more serious, and the treatment and recycling of waste water have been paid more attention by people. [0003] Physical or chemical treatment of COD in high-saline wastewater is costly and may cause secondary pollution. Traditional biological methods have great advantages in treating low-salinity wastewater, but in high-salt, high-acid-base, high-temperature or low-temperature adverse environments, the degradation ability of microorganisms ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 郭志华高会杰孙丹凤赵胜楠李宝忠
Owner CHINA PETROLEUM & CHEM CORP
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